Method for preparing 1,3-propylene glycol by controlling pH value with polyethylene polyamine and fermenting
A polyethylene polyamine and ethylene polyamine technology, applied in the field of bioengineering, can solve the problems of large waste water, low recovery rate, and large energy consumption, and achieve the effects of low preparation cost, high yield, and low loss
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example 1
[0037] Seed preparation: take the 1,3-propanediol strain Klebsiella and insert it into the seed medium, cultivate it at 30-40°C and a stirring speed of 80-110rpm for 8-15 hours, and then transfer it to the second-generation medium. The pH is 7.5-9.0, and the temperature is 30-40° C. and cultured for 5-12 hours, and the secondary seeds are prepared for use.
[0038] Fermentation culture: use industrial glycerol as the carbon source, the loading capacity is 80%, the initial glycerin is 40g / L, nitrogen gas is introduced, the ventilation rate is 0.1-0.5vvm, the secondary seeds with the inoculum size of 10% are inserted into it, and the fermentation temperature 30 ~ 40 ℃, stirring speed 80 ~ 110rpm for fermentation culture, when the glycerin drops to a certain concentration, add glycerin, add 50% tetraethylenepentamine aqueous solution to control the pH of the fermentation liquid to 6.8, when no acid is produced Stop fermentation, final liquid chromatography analysis, chromatograph...
example 2 4
[0043] Others are the same as Example 1, and the experiment is repeated with concentration of 60% diethylenetriamine, 70% triethylenetetramine or 50% n, n', n"-trimethyldiethylenetriamine aqueous solution instead of tetraethylenepentamine aqueous solution.
[0044] The analysis results of the fermentation broth are listed in Table 1.
[0045]
example 5
[0047] The fermented liquid obtained by Example 1 is filtered to obtain the extracted mother liquor (containing 1,3-propanediol 85g / L, lactic acid 8g / L, acetic acid 6g / L, ethanol 5g / L) after the thalline is filtered out, and the extracted mother liquor 100kg is obtained by polyamide Nanofiltration membrane desalination, desalination pressure 1MPa, after the mother liquor permeates 80kg, slowly add 20kg of dialysis water, desalination by nanofiltration membrane, and finally obtain 100kg of dialysate, the final liquid chromatography analysis, the chromatographic column is Carbohydrate column, flow The phase is 0.005mol / L H 2 SO 4 , the flow rate is 0.5ml / min, the column temperature is room temperature, and the injection volume is 20μl. The detector is a Beckman 126 refractive index detector. The concentration of 1,3-propanediol in the dialysate is 84g / L, the concentration of lactic acid is 0g / L, the concentration of acetic acid is 0g / L, and the concentration of ethanol is 5g / L....
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