A kind of extraction method of total coumarin and the application of extracted total coumarin

An extraction method, the technology of total coumarin, applied in the field of application of total coumarin, to achieve the effects of high content of active ingredients, small toxic and side effects, and a wide range of applications

Inactive Publication Date: 2011-12-28
SHANDONG ACAD OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Allopurinol has certain limitations in the prevention and treatment of gout. Only when the accumulated uric acid is obvious in the circulation can allopurinol hinder the biosynthesis of uric acid, and it will produce toxic side effects such as skin allergies, hepatitis, threats Allergic Syndrome of Life, etc.
Long-term use of benzbromarone has toxic side effects on the kidneys, which limits its safe use to a certain extent

Method used

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  • A kind of extraction method of total coumarin and the application of extracted total coumarin
  • A kind of extraction method of total coumarin and the application of extracted total coumarin
  • A kind of extraction method of total coumarin and the application of extracted total coumarin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The extraction solvent used in this example is 70% ethanol, which is extracted by hot reflux for 3 times, each time for 2 hours.

[0036] Its preparation method is:

[0037] a) After removing the impurity from the seed coat of the seed coat of the seedling, it is crushed into a coarse powder of 20-40 mesh. Take 100g of powder, put it in a Soxhlet extractor, and carry out Soxhlet degreasing with petroleum ether with a boiling range specification of 30-60°C for 3 hours until the petroleum ether extract is colorless, discard the petroleum ether extract, and evaporate the petroleum ether from the residue.

[0038] b) Add 1000mL of 70% ethanol to the residue and extract under reflux for 3 times, each time for 2 hours, filter, and recover the ethanol from the filtrate under reduced pressure to obtain the crude total coumarin.

[0039] c), adding 1% potassium hydroxide solution to the obtained total coumarin crude extract, stirring while adding, adjusting pH=7, filtering, dis...

Embodiment 2

[0041] The extraction solvent used in this example is 80% ethanol, which is extracted by hot reflux, and extracted twice for 2 hours each time.

[0042] Its preparation method is:

[0043] a) After removing the impurity from the seed coat of the seed coat of the seedling, it is crushed into a coarse powder of 20-40 mesh. Take 100g of powder, put it in a Soxhlet extractor, and carry out Soxhlet degreasing with petroleum ether with a boiling range specification of 60-90°C for 3 hours until the petroleum ether extract is colorless, discard the petroleum ether extract, and evaporate the petroleum ether from the residue.

[0044] b) Add 1000mL of 80% ethanol to the residue, heat and reflux for extraction twice, each time for 2 hours, filter, and recover ethanol from the filtrate under reduced pressure to obtain crude total coumarin.

[0045] c), add 2% potassium hydroxide to the obtained crude extract, stir while adding, adjust pH=8, filter, discard insoluble matter, add 2% hydroc...

Embodiment 3

[0047] The extraction solvent used in this example is 95% ethanol, which is extracted by hot reflux for 3 times, 2 hours each time.

[0048] Its preparation method is:

[0049] a) After removing the impurity from the seed coat of the seed coat of the seedling, it is crushed into a coarse powder of 20-40 mesh. Take 100g of powder, put it in a Soxhlet extractor, degrease with petroleum ether with a boiling range specification of 30-60°C for 3 hours until the petroleum ether extract is colorless, discard the petroleum ether extract, and evaporate the petroleum ether from the residue.

[0050] b) Add 1000 mL of 95% ethanol to the residue after degreasing, heat and reflux to extract 3 times, each time for 2 hours, filter, and recover ethanol from the filtrate under reduced pressure to obtain crude total coumarin.

[0051] c), add 1% potassium hydroxide to the obtained crude extract, stir while adding, adjust pH=7, filter, discard insoluble matter, add 1% hydrochloric acid to the f...

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Abstract

The invention, belonging to the technical field of extracting effective components of Chinese medicines, provides a method of extracting total coumarin from seed coats of caper euphorbia seeds, comprising the following steps: crushing the seed coats of caper euphorbia seeds into coarse powder, using petroleum ether to degrease to obtain the residue; carrying out extraction on the residue with ethanol or methanol to obtain the crude product of total coumarin; adding potassium hydroxide, adjusting the pH value to 7-8, filtering, adding hydrochloric acid, adjusting the pH value to 2-3, keeping in cold storage at the temperature of 3-5 DEG C for 12-16 h, carrying out extraction filtration, throwing away the filtrate, and carrying out vacuum drying to obtain the total coumarin. The obtained total coumarin can be used for preparing medicines for curing hyperuricemia. The total coumarin extracted from the seed coats of caper euphorbia seeds, so that the resource can be fully utilized, the utilization rate of caper euphorbia seeds is improved, and the yield is increased. The method has the advantages of simple extraction method, low cost, easy operation, and good repeatability, and the total coumarins has high purity of more than 80 %. The total coumarin has good treatment effect on hyperuricemia, and has high content of the effective components, good drug stability, outstanding effect of lowering uric acid, wide application range, and little side effects.

Description

technical field [0001] The invention belongs to the technical field of extracting active ingredients of traditional Chinese medicines, and in particular relates to an extraction method for extracting total coumarin from the seed coat of Stephania chinensis, and also relates to the application of the extracted total coumarin. Background technique [0002] Coumarin compounds are secondary metabolites that are ubiquitous in nature, mainly containing teretin, teretin, daphnetin and other components. teretin can reduce the serum uric acid level in animals with hyperuricemia, and carrageenin Glue, dextran, 5-HT and histamine can inhibit the arthritis in rats, and can also inhibit the erythema reaction caused by ultraviolet radiation on the back of guinea pigs, the formation of rat granuloma (cotton ball method), and the capillary inflammation caused by histamine. Increased permeability. Percetulin can significantly inhibit the increase of capillary permeability induced by hista...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/47A61P19/06A61K131/00
Inventor 李英霞刘瑾侯立静李晓晶曲永胜
Owner SHANDONG ACAD OF CHINESE MEDICINE
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