Preparation method of znse quantum dot labeled bovine serum albumin fluorescent probe

Abstract

The invention discloses a method for preparing a ZnSe quantum dot marked bovine serum albumin fluorescent probe, which comprises the following steps of: A, preparing KH4Se solution by reducing selenium powder through potassium borohydride; B, respectively weighing the potassium borohydride and the selenium powder, placing the potassium borohydride and the selenium powder into a test tube with a plug, adding high purity water into the test tube, and plugging the test tube with the plug to obtain colorless clear liquid, i.e. potassium selenium hydride; C, weighing zinc acetate crystals and reduced glutathione and dissolving the zinc acetate crystals and the reduced glutathione in oxygen-free high purity water; D, under the condition of stirring and continuously filling high purity argon, adding the potassium selenium hydride and regulating a pH value by utilizing sodium hydroxide solution until raw materials are mixed; E, separately charging the mixture into polyfluortetraethylene digestion tanks and heating the polyfluortetraethylene digestion tanks, wherein a molar ratio of KH4Se to Zn(Ac)2.2H2O to the reduced glutathione is (1 / 15-1 / 10):1:(1.6-1.8); and F, taking ZnSe quantum dots and bovine serum albumin, adding ethyl [3-(dimethylamino)propyl] carbodiimide hydrochloride and oscillating at constant temperature to obtain the quantum dot marked bovine serum albumin fluorescent probe. The method has simple process, is easy to control. The ZnSe quantum dot marked bovine serum albumin fluorescent probe has good biocompatibility and detection performance and can be well used for fluorescence detection.

Description

technical field [0001] The invention relates to the biological application technical field of quantum dots, and more specifically relates to a preparation method of a ZnSe quantum dot-labeled bovine serum albumin fluorescent probe, which can be used to detect pollutants, poisons and interaction effects with proteins. Background technique [0002] Quantum dots synthesized in the organic phase are not hydrophilic, so they cannot be directly applied to biological systems, and must be modified on the surface. The operation process is complicated and the conditions are high. Quantum dots prepared by aqueous phase synthesis can be directly used in biological probes, and more and more attention has been paid to their research, and the research on this aspect is progressing rapidly. However, since the ligands selected for the quantum dots synthesized in the water phase are mostly simple mercapto compounds, the active groups on the surface are relatively small and the surface area ...

AI Technical Summary

Problems solved by technology

However, since the ligands selected for the quantum dots synthesized in the water phase are mostly simple mercapto compounds, the active groups on the surface are relatively small and the surface area is small, and it is difficult to combine with the target substance, so the direct application of the quantum dots in the water phase has limitations.