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Bacillus thuringiensis GL-1 and application thereof in phosphate solubilization and disease prevention of soil

A technology of Bacillus aureus and microbial strains, applied in the fields of application, bacteria, fungicides, etc., can solve the problems of the antagonistic function of single wilt and bacterial wilt, the ability of single phosphate-solubilizing bacteria to dissolve phosphorus, etc., and improve the utilization rate of phosphorus fertilizer. , good antagonism effect, the effect of improving the effectiveness

Inactive Publication Date: 2012-01-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, domestic and foreign studies on phosphate-solubilizing bacteria, antagonistic bacteria against banana wilt and tomato bacterial wilt are carried out steadily, but most of these studies are limited to the single phosphorus-solubilizing ability of phosphate-solubilizing bacteria, or the single antagonistic function of fusarium wilt and bacterial wilt
However, while having high-efficiency phosphorus-solubilizing effect, the multi-functional strain that can produce antagonistic effects on Fusarium wilt of banana and R. solanacearum of tomato has never been reported.

Method used

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  • Bacillus thuringiensis GL-1 and application thereof in phosphate solubilization and disease prevention of soil
  • Bacillus thuringiensis GL-1 and application thereof in phosphate solubilization and disease prevention of soil
  • Bacillus thuringiensis GL-1 and application thereof in phosphate solubilization and disease prevention of soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and purification of bacterial strains

[0032] (1) Separation

[0033]Prepare insoluble inorganic phosphorus medium: glucose 10.0g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, ferrous sulfate heptahydrate 0.03g, manganese sulfate 0.03g, yeast extract 0.4g, calcium phosphate 5g , distilled water 1000ml, P H 7.0~7.5.

[0034] Taking the soil developed by the limestone parent material in Mochuan Township, Xing'an County, Guilin City, Guangxi Zhuang Autonomous Region as the screening soil sample, 10.0 g of the screening soil sample was accurately weighed by the flat plate coating method, and put into a 250 ml triangular flask with 90 ml of sterile water (plus glass beads), shaking on a shaker for 30 minutes to fully disperse the cells, let it stand for 20-30 seconds, and take the supernatant for 10-fold decreasing dilution, using 10 3 ~10 5 Dilution, use a pipette gun to pipette 0.1 mL of the dilution respectively, spread...

Embodiment 2

[0037] Example 2 Characterization

[0038] (1) Morphological characteristics of bacteria

[0039] Gram-positive bacteria with spores. The bacteria are rod-shaped, motile, and obligately aerobic. Gram-stained micrographs of bacteria figure 1 , see photos of spore staining figure 2 .

[0040] (2) Colony Morphological Characteristics

[0041] After 24 hours of culture on beef extract peptone medium, the colonies formed are round or irregular. After 48 hours, the colonies are all round, milky white, about 2-3mm in diameter, with irregular edges, flat and moist.

[0042] (3) Growth characteristics

[0043] In the liquid medium of 5g of beef extract, 5g of peptone, 5g of sodium chloride, and 1L of water, the rotation speed was 113 rpm, the temperature was 30°C, and the initial pH value was 7.0, and the cultured for 18 hours, the number of viable bacteria was 2.91 ±0.60×10 8 cfu / ml.

[0044] (4) Physiological and biochemical characteristics

[0045] The Biolog Ecoplate ...

Embodiment 3

[0051] Example 3 Bacillus thuringiensis Growth characteristics of GL-1 strain in different fermentation medium conditions

[0052] Glucose, corn flour, wheat bran, vegetable starch, peanut bran, and rice bran were used as the main components of the medium, and the concentration was 20 g / L. The other medium components were the same, beef extract 5g / L, peptone 5g / L, sodium chloride 5g / L. Use 1mol / L NaOH or HCl to adjust the pH to 7.1~7.5. Put 45ml of each treatment medium in a 250ml Erlenmeyer flask, sterilize, and repeat three times for each group of culture medium. According to the 10% inoculum amount, GL-1 bacterial classification was inoculated into the above-mentioned sterilized fermentation medium, cultivated for 24 hours at a temperature of 33 ° C and a rotating speed of 120 rpm, and measured the number of live bacteria in each treatment fermentation liquid by plate colony counting method. Two dilution gradients with three replicates for each dilution. The measure...

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Abstract

The invention discloses a Bacillus thuringiensis GL-1 and application thereof in phosphate solubilization and disease prevention of soil. The strain collection number is CGMCC No.4923, and the strain is collected in China General Microbiological Culture Collection (CGMCC) on July 21th, 2011. The Bacillus thuringiensis GL-1 disclosed by the invention can enhance the effectiveness of insoluble phosphorus in soil and the utilization ratio of phosphate fertilizer, and can promote plant growth on the premise of reducing the consumption of phosphate fertilizer. In addition, the strain has favorable antagonistic effects on pathogenic bacteria of Banana vascular wilt and tomato bacterial wilt, can effectively inhibit growth and reproduction of pathogenic bacteria Fusarium oxysporum and Pseudomonas solanacearum, and can be used as a biological pesticide. Besides, the strain can also enhance the microbial diversity and microbial abundance in soil. The biological fertilizer prepared from the strain can prevent soil-borne disease and promote plant growth, and thus, has wide application prospects.

Description

Technical field [0001] The present invention involves a microorganism and its application, which specifically involves a kind of Su Yun Golden Bybalobacteria ( Bacillus thuringiensis ) GL-1 and its applications in soil disintegration (banana wilt and tomato blight) and improve the application of soil micro-ecological structures. Background technique [0002] Phosphorus is one of the nutritional elements necessary for plant growth and development. The amount of phosphorus absorption of crops is significantly positively correlated with its biomass (R = 0.97) and yield (R = 0.75).However, due to the form of phosphorus fixed existence, soil shortage of phosphorus is widespread worldwide, and insufficient phosphorus supply is one of the most important restrictions in the world's agricultural production.From the perspective of our country, 74%of the country ’s cultivated soil lacks phosphorus.There are two main ways to solve this problem: one is to increase the investment in phosphorus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C05F11/08A01N63/02A01P3/00A01P1/00C12R1/07
Inventor 蔡燕飞李永涛蔺中孔德颖
Owner SOUTH CHINA AGRI UNIV
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