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Fresh animal medicinal composition and method for measuring content of Chinese medicines of fresh animal medicinal composition

A determination method and composition technology, which can be used in measurement devices, material separation, and analysis of materials, etc., and can solve problems such as lack of quality control research and poor specificity.

Active Publication Date: 2012-02-01
BEIJING JIANSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The drug standard (WS3-158(Z-036)-2001Z) promulgated by Jinlong Capsules controls the quality of its products, including: traits, identification, inspection and content determination. From the current SFDA requirements for quality control standards for traditional Chinese medicines, As well as the status quo of chemical and pharmacological research on Jinlong Capsule raw materials fresh Shougong, fresh white snake, and fresh Viper, the current content determination items use visible spectrophotometry, Folin-phenol reagent to determine total protein, the specificity of this method In addition, there is no quality control research on amino acids, free monosaccharides, polysaccharides and some endogenous small molecular life basic substances commonly found in animal medicines

Method used

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  • Fresh animal medicinal composition and method for measuring content of Chinese medicines of fresh animal medicinal composition
  • Fresh animal medicinal composition and method for measuring content of Chinese medicines of fresh animal medicinal composition
  • Fresh animal medicinal composition and method for measuring content of Chinese medicines of fresh animal medicinal composition

Examples

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Effect test

experiment example

[0136] Experimental Example: Quantitative Analysis of Amino Acids in the Pharmaceutical Composition Jinlong Capsules of the Present Invention and Various Raw Materials

[0137] 1. Experimental materials and equipment

[0138] 1.1 Experimental materials: 17 amino acids (aspartic acid, glutamic acid, serine, histidine, glycine, threonine, arginine, alanine, tyrosine, cystine, valine, methionine , phenylalanine, isoleucine, leucine, lysine and proline) mixed standard solution, borate buffer solution, OPA and FMOC derivatization reagents were purchased from Agilent; methanol and acetonitrile were purchased from B&J Company Provided, chromatographically pure; sodium dihydrogen phosphate, hydrochloric acid and sodium hydroxide are all analytically pure, purchased from Beijing Chemical Plant; the pharmaceutical composition Jinlong Capsule of the present invention (prepared according to the method of Example 1) and the raw materials of the pharmaceutical composition of the present inv...

Embodiment 1

[0242] The assay method of embodiment 1 pharmaceutical composition capsule of the present invention

[0243] 1. Preparation of capsules

[0244] 1500kg of fresh Shougong, 750kg of fresh white snake and 750kg of fresh Viper, cut fresh Shougong, fresh white snake and fresh Viper into minced meat, grind it into a paste, add an equal amount of water to homogenate, and place Freeze at -25°C for 24 hours, take it out, put it in a 37°C water bath to melt, repeat three times, filter, the filtrate is centrifuged at 8000 rpm, absorb the supernatant, ultrafilter, take the filtrate and add 172kg of β-cyclodextrin for inclusion , the filtrate is vacuum freeze-dried, crushed into fine particles, dried at low temperature, and made into capsules;

[0245] 2. Content determination

[0246] (1) Preparation of standard solution:

[0247] a. 0.1mol / L hydrochloric acid solution: Take 0.83mL of 37.5% concentrated hydrochloric acid in a 100mL measuring bottle, dilute with water and set the volume...

Embodiment 2

[0267] Embodiment 2 The method for assaying the content of the raw material drug of the pharmaceutical composition of the present invention, fresh snake snake

[0268] 1. Preparation of fresh Snake japonica powder

[0269] Take 3000g of fresh white snake, chop into minced meat, grind into a paste, add an equal amount of water to homogenate, freeze at -25°C for 24 hours, take it out, put it in a water bath at 37°C to melt, repeat three times, filter, The filtrate was centrifuged at 8000 rpm, the supernatant was absorbed, ultrafiltered, the filtrate was added with 172 g of β-cyclodextrin for inclusion, the filtrate was vacuum freeze-dried, crushed into fine particles, and dried at low temperature to make a powder.

[0270] 2. Content determination

[0271] (1) Preparation of standard solution: (same as Example 1)

[0272](2) preparation of need testing solution

[0273] a, the preparation of free amino acid need testing solution: get raw material drug of the present invention...

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Abstract

The invention discloses a fresh animal medicinal composition and a method for measuring the content of Chinese medicines of the fresh animal medicinal composition. The method comprises the following steps of: putting the fresh animal medicinal composition and the Chinese medicines into a measuring flask, dissolving by adding water, performing ultrasonic extraction and centrifugation, taking supernate, and sieving the supernate with a microporous filtering film to obtain a free amino acid test sample solution; and weighing the fresh animal medicinal composition and the Chinese medicines, fullydissolving by adding water, then performing ultrasonic extraction and centrifugation, taking the supernate, putting the supernate into an ampoule bottle, adding hydrochloric acid, performing vacuum nitrogenization and hydrolyzation, taking the mixture out, cooling the mixture to room temperature, adding a NaOH solution, uniformly mixing, transferring to the measuring flask, diluting by adding water, and fixing the volume to a scale, uniformly mixing, centrifuging, taking the supernate, and sieving the supernate with the microporous filtering film to obtain the total amino acid test sample solution. A chromatographic column is used for detecting primary amino acid and secondary amino acid. A mobile phase A comprising acetonitrile, methanol and water and a mobile phase B comprising Na2HPO4 of which the pH is 7.8 are used for performing gradient elution. The method is simple in operation and high in measurement resolution and sensitivity, and by the method, 12 to 26 types of amino acids in the fresh animal medicinal composition and the Chinese medicines thereof can be separated in a short time, so the method is a reliable method for measuring the amino acid content.

Description

technical field [0001] The invention relates to an assay method, in particular to a method for assaying the content of a fresh animal drug composition and a raw material medicine for treating primary liver cancer with blood stasis syndrome. Background technique [0002] The Jinlong Capsule of the present invention (Z10980041) is a medicine for treating cancer. It is a compound preparation formulated with medicinal animals (fresh shougong, fresh Viper, and fresh golden snake), and has definite curative effect on antitumor. [0003] The drug standard (WS3-158(Z-036)-2001Z) promulgated by Jinlong Capsules controls the quality of its products, including: traits, identification, inspection and content determination. From the current SFDA requirements for quality control standards for traditional Chinese medicines, As well as the status quo of chemical and pharmacological research on Jinlong Capsule raw materials fresh Shougong, fresh white snake, and fresh Viper, the current cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/28G01N30/06
Inventor 李建生
Owner BEIJING JIANSHENG PHARMA
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