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Pharmaceutical composition containing extracts of ilex latifolia as active ingredient

A technology of holly vulgaris and active ingredients is applied in the directions of drug combinations, medical preparations containing active ingredients, functions of food ingredients, etc., which can solve the problems of unproven treatment effects of degenerative brain diseases, etc., and achieve the effect of inhibiting memory damage.

Inactive Publication Date: 2012-03-14
HANKOOK PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the therapeutic effect of Ilex japonicus on degenerative brain disorders has not actually been proven

Method used

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  • Pharmaceutical composition containing extracts of ilex latifolia as active ingredient
  • Pharmaceutical composition containing extracts of ilex latifolia as active ingredient
  • Pharmaceutical composition containing extracts of ilex latifolia as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The manufacture of embodiment 1 large-leaf holly (Ilex latifolia) extract

[0052] Production of ethanol extract

[0053] The big-leaf holly is chopped and used after being purchased at the Chinese herbal medicine market in Gyeongdong, Seoul. After preparing 100 g of Ilex japonica, it was extracted three times in a reflux condenser within three hours using 3 L of 100% ethanol at room temperature. The above solution was filtered through Waterman No. 1 paper and then dried to finally harvest 18.7 g of ethanol extract. The finally harvested holly extract was dissolved in dimethylsulfoxide (DMSO) to a concentration of 50 mg / ml, and stored at -20°C. During the experiment, the experiment buffer solution was diluted so that the final concentration of DMSO was less than 0.1% before use.

[0054] Manufacture of water extract

[0055] Except that water was used as the extraction medium instead of 100% ethanol, the powder (yield 21.5%) was obtained according to the extracti...

Embodiment 2

[0060] Example 2 Confirmation of Cerebral Infarction and Edema Suppressive Effects of Ilex japonicus Extract in Rats Induced Cerebral Infarction

[0061]The inventors of the present invention conducted the following experiment in order to confirm the efficacy of the extract of Ilex japonicus on rats with cerebral infarction. First, 300±5 g SD rats (SAMTAKO, Gyeonggi-do) were anesthetized by inhalation of 1-2% isoflurane (N2:O2=4:1), and the right carotid artery was carefully exposed surgically. In rats, the linguinal artery branching from the outer diameter artery to the central part and the thyroid artery (thyroid artery) branching from the external carotid artery to the internal carotid artery were ligated by electrocautery. At this time, 30 minutes before arterial ligation, one hour after ligation, and one hour after removal of the ligation, holly extracts (50, 100, or 200 mg / kg, respectively) or MK-801 (1 mg / kg) as a positive control group were fed. / kg). Rats injected...

Embodiment 3

[0066] Example 3 Passive avoidance test (passive avoidance test; Step through) memory and determination of learning in rats and mice

[0067] As experimental animals, ICR mice (BioLink Co. Korea) were used. After a period of adaptation period after purchase, they were fed with holly (25, 50 or 100 mg / kg) on ​​the first day of the experiment (day 0), and after 30 minutes 15 μl (15 nmol) of 1 mM Aβ(25-35) (Bachem, Switzerland) was slowly injected into the ventricles of the brain using a microsyringe equipped with a 27-gauge nuddle (Maurice T et al., Brain Research, 1996, 706: 181-193 ). Within one week thereafter, 25, 50, and 100 mg / kg of Ilex japonicus extract were fed once a day, and the animals were placed in the bright room of a Step through apparatus (Gemini II avoidance system, Sandiego Instrument). In the box, three open field tests were carried out. On the 7th day, feed the Ilex japonicus extract (25, 50 or 100mg / kg), place the animal in the bright box after 30 minut...

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Abstract

The invention relates to a component containing extracts of ilex latifolia as active ingredients and used for preventing or curing an ischemic disease or a degenerative brain disease. In particular, extracts of ilex latifolia taking water, alcohol or a mixture of water and alcohol as a menstruum restrain the cerebral infarction and dropsy on a rat having the cerebral infarction, and restrain the memory impairment on a rat having neurotoxicity caused by being injected an amyloid beta protein(amyloid beta protein; A beta(25-35)), and according to the injection volume, the oxidative stress caused by hydrogen peroxide, the excitatory amino acid glutamic acid, the A beta(25-35) causing the neurotoxicity, the death of cortical brain nerve cells caused by hypoxidosis etc. can be restrained. Therefore, the extracts of ilex latifolia can be used as drug components for preventing or curing the ischemic disease or the degenerative brain disease or used as active ingredients of functional health food.

Description

technical field [0001] The present invention relates to a pharmaceutical composition for the prevention or treatment of ischemic disease or degenerative brain disease, and specifically, contains a plant extract as an active ingredient. Background technique [0002] Cerebral apoplexy (cerebral apoplexy), ie, cerebral infarction, is mainly caused by cerebral arterial thrombosis or embolism caused by vascular arteriosclerosis and cardiogenic embolism caused by heart disease or the like. Cerebral infarction caused by cerebrovascular blockage is further divided into cerebral thrombosis and cerebral embolism. Cerebral thrombosis is arteriosclerosis caused by high blood pressure, diabetes, and hyperlipidemia. The arterial wall becomes thicker or harder, resulting in narrowing of the blood vessel. The inner wall of the blood vessel is easily damaged and rough, and the blood flow is not smooth. Reduced or disrupted, brain cells are deprived of oxygen and nutrients, leading to brain ...

Claims

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Application Information

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IPC IPC(8): A61K36/185A23L1/29A23L1/30A61P9/10A61P25/14A61P25/28A23L33/00
CPCA23L33/105A23V2200/322A23V2250/21A61K36/185A61K2236/333A61P9/10A61P25/00
Inventor 成莲姬裴起焕刘载国金镇杓
Owner HANKOOK PHARM
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