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Production process for compounded culture medium

A production process and culture medium technology, which is applied in the direction of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve the problems of unclear composition of culture medium and unstable quality, and achieve the benefits of three wastes treatment, low product cost and high fermentation level Improved effect

Inactive Publication Date: 2012-06-20
QINGDAO CREAT MEDIUM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem of unclear composition and unstable quality of traditional medium, and to provide a production process of compound medium by adopting protein compounding technology and homogenization deployment technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Production of cottonseed protein

[0030] The cottonseed is peeled into 2 to 4 petals with a toothed bar peeling machine, and then the kernel shell is separated. The material after peeling is separated by a three-layer stepped separation sieve to separate the cotton kernel. The shell content of the separated kernel is less than 8%. The kernel content in the shell is less than 0.5%. Use a drum-type softening pot to soften the separated cotton kernels and adjust the water content. The softening temperature is 80℃. After softening, the embryo is rolled. The thickness of the embryo is 0.4mm. The embryo is flat-baked. Dry machine drying, drying temperature 85℃, after drying, the moisture content of cotton kernel is less than 6%, then use No. 6 solvent oil to remove cottonseed oil, and use methanol to dephenol, dry the dephenolic material to produce detoxified cottonseed protein;

[0031] 2) Production of peanut protein / soy protein

[0032] The peanuts / soybeans are cleaned, dri...

Embodiment 2

[0038] 1) Production of cottonseed protein

[0039] The cottonseed is peeled into 2 to 4 petals with a toothed stick peeling machine, and then the kernel shell is separated. The material after peeling is separated by a three-layer stepped separation sieve. The shell content of the separated kernel is less than 8%. The kernel content in the shell is less than 0.5%. Use a drum-type softening pot to soften the separated cotton kernels and adjust the water content. The softening temperature is 60℃. After softening, the embryo is rolled. The thickness of the embryo is 0.3mm. The embryo is flat-baked Dry machine drying, drying temperature 85℃, after drying, the moisture content of cotton kernel is less than 6%, then use No. 6 solvent oil to remove cottonseed oil, and use methanol to remove phenol, and dry the dephenolic material to produce detoxified cottonseed protein;

[0040] 2) Production of peanut protein / soy protein

[0041] The peanuts / soybeans are cleaned, dried, cooled, peeled, ...

Embodiment 3

[0047] 1) Production of cottonseed protein

[0048] The cottonseed is peeled into 2 to 4 petals with a toothed stick peeling machine, and then the kernel shell is separated. The material after peeling is separated by a three-layer stepped separation sieve. The shell content of the separated kernel is less than 8%. The kernel content in the shell is less than 0.5%. Use a drum-type softening pot to soften the separated cotton kernels and adjust the water content. The softening temperature is 70℃. After softening, the embryo will be rolled. The thickness of the embryo is 0.3mm. Dry machine drying, drying temperature 85℃, after drying, the moisture content of cotton kernel is less than 6%, then use No. 6 solvent oil to remove cottonseed oil, and use methanol to remove phenol, and dry the dephenolic material to produce detoxified cottonseed protein;

[0049] 2) Production of peanut protein / soy protein

[0050] The peanuts / soybeans are cleaned, dried, cooled, peeled, and ground, and then...

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Abstract

The invention relates to a production process for compounded culture medium finely and deeply processed with agricultural products such as cottonseed, soybean, peanut and the like as raw materials, wherein the compounded culture medium is suitable for microbial growth and metabolism. The invention provides a following technical scheme that: the production process for the compounded culture mediumcomprises the steps of producing cottonseed protein, producing peanut protein / soybean protein, compounding for the first time and compounding for the second time. The production process for the compounded culture medium disclosed by the invention has the following beneficial effects that: 1) the culture medium of the invention meets the microbial requirement in the fermentation process and can beeffectively utilized, the redundant part produced in the fermentation culture can be prevented from entering the fermentation waste liquid and the clean production can be realized; 2) with the culture medium of the invention, the problems of low fermentation titer, unstable production and high pollution level of fermentation waste liquid caused by too many kinds of nitrogen sources added and great quality fluctuation in the fermentation production process are solved; and 3) the culture medium of the invention comply with the target microbial growth and metabolism characteristics and has the advantages of good microbial utilization effect, low product cost, improved production quality and stable production level.

Description

Technical field [0001] The invention relates to using agricultural products such as cottonseeds, soybeans, peanuts and the like as raw materials for fine and deep processing to produce a compound medium suitable for microbial growth and metabolism. Background technique [0002] The total annual production of antibiotics in my country is about 50,000 tons. The production of these antibiotic raw materials is produced by industrial fermentation, and the total demand for nitrogen source medium is more than 100,000 tons. The output of my country’s three major fermentation products is about 1 million tons, enzyme preparations such as amylase, protease, cellulase, glucoamylase, etc., and amino acids such as lysine, L-alanine, glutamic acid and aspartic acid. Fermentation production requires a large amount of culture medium. The annual output of biological pesticides such as Jinggangmycin, Bacillus thuringiensis, abamectin and other products is hundreds of thousands of tons, and the amou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
Inventor 张术臻刘希军
Owner QINGDAO CREAT MEDIUM
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