Enzyme particle coated with alkali protease and preparation method thereof

A technology of enzyme granules and protease, which is applied in the field of enzyme granules coated with alkaline protease and its preparation, which can solve the problems of rough and irregular granules, poor resistance to heat and humidity, and slow disintegration speed, and achieve fast dissolution and decontamination effects Good, long shelf life effect

Active Publication Date: 2012-07-04
QINGDAO VLAND BIOTECH GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing alkaline protease granule products generally have problems such as rough and irregular particles, poor roundness, slow disintegration speed, poor heat and humidity resistance, short shelf life, and weak detergency.

Method used

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  • Enzyme particle coated with alkali protease and preparation method thereof
  • Enzyme particle coated with alkali protease and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Mix 9100g of starch, 980g of microcrystalline cellulose, 980g of dextrin, 1400g of sodium sulfate, 1470g of sucrose, and 70g of sodium dodecylsulfonate in a mixer for 30 minutes, add an appropriate amount of water to make a wet material, and put it in a twin-screw extruder Medium (screw speed 40rpm) extruded into strips. Put the strip-shaped material into the rounding machine, set the speed at 600rpm, and set the fan at 10Hz, until the strip-shaped material is processed into round pellets and then discharged. Place the pellets in a fluidized bed, dry them under the condition of 40°C air inlet until the material temperature is 35°C, and then release the dried pellets to obtain blank pellet cores.

[0021] 5000g liquid alkaline protease (converted to dry weight 857g), 429g dextrin, 143g boric acid are made into the suspension solution that mass volume ratio is 5%, the blank ball core 10kg that will prepare is dropped in fluidized bed, start fluidized bed and Coating equi...

Embodiment 2

[0023] Mix 7000g of starch, 2100g of microcrystalline cellulose, 2100g of dextrin, 2100g of sodium sulfate, 420g of sucrose, and 280g of sodium dodecylsulfonate in a mixer for 30 minutes, add an appropriate amount of water to make a wet material, and extrude it in a vertical spinning press. Extrude into strips in the machine (feeding 30Hz, extrusion 45Hz). Put the strip-shaped material into the rounding machine, set the speed at 1000rpm, and set the fan at 20Hz, until the strip-shaped material is processed into round pellets and then discharged. Place the pellets in a fluidized bed, dry them under the condition of 60°C air inlet until the material temperature is 53°C, release the dried pellets, and obtain blank pellet cores.

[0024] 1477g of alkaline protease powder, 277g of dextrin, and 92g of boric acid are made into a suspension with a mass volume ratio of 7%, and 10 kg of prepared blank ball cores are dropped into the fluidized bed, and the fluidized bed and coating equip...

Embodiment 3

[0026] Mix 7420g of starch, 1120g of microcrystalline cellulose, 1120g of dextrin, 1540g of sodium sulfate, 2100g of sucrose, and 700g of sodium dodecylsulfonate in a mixer for 30 minutes, add an appropriate amount of water to make a wet material, and put it in a screw extruder (screw speed 50rpm) extruded into strip material. Put the strip-shaped material into the rounding machine, set the rotating speed at 1400rpm, and the fan at 30Hz, until the strip-shaped material is processed into round pellets and then discharged. Place the pellets in a fluidized bed, dry them under the condition of 80°C air inlet until the material temperature is 70°C, release the dried pellets, and obtain blank pellet cores.

[0027] 1875g alkaline protease powder, 425g dextrin, and 200g boric acid are made into a suspension with a mass volume ratio of 8%, and 10kg of blank ball cores are dropped into the fluidized bed, and the fluidized bed and coating equipment are started. When the air inlet is 80...

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Abstract

The invention relates to an enzyme particle coated with alkali protease and a preparation method thereof. The enzyme particle comprises a blank pellet core and an enzyme layer wrapped outside the blank pellet core. The enzyme particle also comprises an isolation layer and a protection layer which are wrapped on the enzyme layer. The enzyme particle coated with the alkali protease is round and hasno foreign odor and uniform particle size; 20-40 meshes of particles account for at least 90 percent. All properties of the enzyme particle are superior to abroad like products and are shown as follows: the unit weight is 700-900g, i.e. the enzyme particle has very high degree of mixing with washing powder; the enzyme activity of the enzyme particle is as high as 250000U / g-400000U / g; the dissolution velocity is high; the retention period is long, i.e. the enzyme particle can be stored for 6 months at the temperature of 25 DEG C, and the enzyme activity loss is less than 5 percent; the resistance to heat and humidity is good, i.e. the enzyme particle can be stored for 3 months under the conditions that the temperature is 37 DEG C and the humidity is 70 percent, and the enzyme activity lossis less than 10 percent; and a decontamination effect is good.

Description

technical field [0001] The invention relates to an enzyme granule coated with alkaline protease and a preparation method thereof, belonging to the technical field of enzyme preparation application. Background technique [0002] Protease is the most important enzyme among industrial enzymes. It can be divided into acid protease, neutral protease and alkaline protease according to its pH value. Alkaline protease, also known as serine protease, is a kind of enzyme suitable for hydrolyzing protein in the range of partial alkali (pH range is generally 9 to 12). It has strong hydrolysis, alkali resistance and heat resistance. It has a good degradation effect on protein stains such as blood stains and gravy. At present, alkaline protease is the most widely used enzyme in the world, and is widely used in washing, leather processing, medical treatment, food processing, feed and chemical industry, and waste treatment and other fields. [0003] In order to solve the problem of water ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/96C11D3/386
Inventor 马俊孝刘伟张赞剑史衍鲁卢彦梅
Owner QINGDAO VLAND BIOTECH GRP
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