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Microscale urinary albumin colloidal gold detection kit and preparation technology thereof

A technology for detecting kits and urinary albumin, which is applied in biological testing, measuring devices, material inspection products, etc., and can solve the problems of unfavorable clinician diagnosis and curative effect observation, high cost, and long duration

Active Publication Date: 2012-07-04
SHANGHAI CHEMTRON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) methods for clinical detection of trace protein in the diagnosis of early renal damage. Timely diagnosis and curative effect observation
[0005] The existing domestic patents related to microalbuminuria detection methods include: application number 01126895.6, which provides a urine protein chip for rapid detection of kidney damage, but it does not affect the preparation process of the chip, detection sensitivity, minimum detection amount, etc. The characteristics are given, which limits the reliability of its application

Method used

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  • Microscale urinary albumin colloidal gold detection kit and preparation technology thereof
  • Microscale urinary albumin colloidal gold detection kit and preparation technology thereof
  • Microscale urinary albumin colloidal gold detection kit and preparation technology thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] The preparation of embodiment 1 trace urine albumin colloidal gold detection kit

[0068] 1. Reagent source:

[0069] Anti-human albumin monoclonal antibody I, anti-human albumin monoclonal antibody II, goat anti-mouse IgG polyclonal antibody (purchased from MAXMED LABORATORIES INC., USA)

[0070] Nitrocellulose film, glass fiber paper (purchased from SARTORIUS, Germany Sartorius Company)

[0071] 2. Preparation steps:

[0072] method 1:

[0073] 1. Preparation of colloidal gold:

[0074] a) Reduction of chloroauric acid solution for the first time: HAuCl of 6ml 0.0164mol / L 4 The aqueous solution was added to 200ml of double-distilled water, heated and boiled for 30 minutes, then stirred slowly and accurately added 50ml of 0.016mol / L trisodium citrate aqueous solution. Cool to room temperature after ultrasonic oscillation for 2 minutes, the frequency of ultrasonic oscillation is 25KHZ, and a colloidal gold original solution with a particle size of about 15nm is pre...

Embodiment 2

[0121] The sensitivity experiment of embodiment 2 trace urine albumin colloidal gold detection kit

[0122] Detection method:

[0123] 1. Take the microalbuminuria colloidal gold detection kit prepared in Example 1, and place the kit on a horizontal platform.

[0124] 2. Take the urine sample to be tested with a sample pipette, and then drop 2-3 drops (about 100 μl) of the sample into the sample hole of the kit, and use a different pipette for each different sample.

[0125] 3. Observation results: Interpret the results 10-15 minutes after adding the sample.

[0126] Test sample:

[0127] Quality control reference substances with human albumin concentrations of 7.5 μg / ml, 10 μg / ml, 15 μg / ml, and 30 μg / ml were prepared as standards.

[0128] Test results:

[0129] Table 1 Kit sensitivity results

[0130]

[0131] The results in Table 1 confirm that the microalbuminuria colloidal gold detection kit prepared by the present invention has a sensitivity of 15 μg / ml, which me...

Embodiment 3

[0132] The specificity experiment of embodiment 3 trace urine albumin colloidal gold detection kit

[0133] Get the detection kit prepared in Example 1, add samples respectively according to the following table and detect, and the detection results are shown in Table 2:

[0134] Table 2 The specificity test results of microalbuminuria colloidal gold detection kit

[0135]

[0136] It can be seen from the above table that the microalbuminuria colloidal gold detection kit of the present invention has no cross-reaction to 500 μg / ml hemoglobin, 20 μg / ml acetaminophen, and 20 μg / ml vitamin C, and has good specificity.

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Abstract

The invention relates to the field of colloidal gold immunization chromatography, and discloses a microscale urinary albumin colloidal gold detection kit. The detection kit comprises a reagent strip, wherein the reagent strip comprises a substrate, filtering sample paper, an immunization colloidal gold paper sheet, an immunization cellulose nitrate film and absorbent paper, wherein the filtering sample paper, the immunization colloidal gold paper sheet, the immunization cellulose nitrate film and the absorbent paper are successively connected with the each other end to end and are fixed on the substrate; and the immunization colloidal gold paper sheet is coated with a human albumin resistant monoclonal antibody I labeled by colloidal gold, and the immunization cellulose nitrate film is provided with a detection line which is coated with a human albumin resistant monoclonal antibody II and a quality control line which is coated with goat anti mouse IgG. The microscale urinary albumin colloidal gold detection kit provided by the invention has the advantages of rapid response, convenience in detection, stable detection result and cost-saving property.

Description

technical field [0001] The invention relates to the field of colloidal gold immunochromatography, in particular to a microalbuminuria colloidal gold detection kit and a preparation process thereof. Background technique [0002] Microalbuminuria (also known as microalbuminuria, Microalbuminuria, MAU) refers to the presence of microalbumin in urine. Albumin is a normal protein in the blood. Under normal physiological conditions, a very small amount of albumin will also appear in the urine, and the excretion is generally within 30mg / day (or 20mg / L). When the albumin in human urine reaches a concentration of 30-300 mg / day or 20-200 mg / L, it is called microalbuminuria, and when the albumin concentration is greater than 300 mg / day or 200 mg / L, it is called macroalbuminuria. [0003] The abnormal appearance of microalbuminuria (MAU) is generally considered to be one of the important clinical signs of renal failure, diabetes and cardiovascular disease complications. Therefore, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/531
Inventor 张国华
Owner SHANGHAI CHEMTRON BIOTECH
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