Method for extracting substances from fermented shrimp head shells

A technology of shrimp head and fermentation supernatant, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve problems such as waste, slow growth rate, and harsh growth conditions

Inactive Publication Date: 2012-07-18
OCEAN UNIV OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, although yeast cells reproduce fast and have high biomass, the content of astaxanthin in the cells is low. In order to increase the production of astaxanthin, a large amount of carbon and nitrogen sources, especially organic nitrogen sources with high prices (yeast extract and peptone) need to be increased. etc.), it is easy to cause waste, increase the cost, and is not conducive to commercial production
[0006] (3) Algae: Although astaxanthin derived from algae has a 100% left-handed (3S-3'S) structure and has the strongest biological activity, the currently known microalgae usually grow slowly and require more Long autotrophic culture cycle, low biomass, harsh growth conditions, high requirements for water quality, environment and light, and large-scale production is still difficult
[0007] (4) Crustaceans: In nature, astaxanthin mainly exists in the shell of aquatic animals (such as shrimp and crab) in a large amount in the form of ester. Although the content is low, the ash and protein in the shell also limit astaxanthin. However, since no better method has been found at present, and the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] Coarsely crush 10 g of raw shrimp heads to 2-10 cm, add water at a solid-to-liquid ratio of 1:9, and add 10 g of glucose. Inoculate the liquid strains activated for 24 hours in skimmed milk powder culture solution at a ratio of 1%, and degrade at 38° C. for 72 hours. Suction filter the fermentation broth. The liquid part is spray-dried to obtain protein calcium powder. The solid part was extracted with ethanol at a solid-to-liquid ratio of 1:6 to extract astaxanthin at 45° C. for 45 minutes, and then centrifuged at 8000 r / min for 20 minutes. Keep the liquid part and repeat the above operation twice for the solid part. Combine the liquid portions. Dry the remaining shrimp head residue to obtain crude chitin. Finally, the obtained astaxanthin oil contained 21.6 μg of astaxanthin, and the organic solvent was recovered; 0.52 g of complex protein peptide powder rich in calcium lactate and amino acids was obtained; and 1.70 g of crude chitin was obtained.

example 2

[0026] 20g of raw shrimp heads are coarsely crushed to 2-10cm, water is added at a solid-to-liquid ratio of 2:8, and 15g of glucose is added. Other operations are the same as example 1. Finally, the obtained astaxanthin oil contained 15.7 μg of astaxanthin, and the organic solvent was recovered; 0.59 g of complex protein peptide powder rich in calcium lactate and amino acids was obtained; and 1.78 g of crude chitin was obtained.

example 3

[0028] Raw material, water ratio and glucose amount are with example 1. The strains were activated for 24 hours and inoculated with 5%. Degradation time 72h, other operations are the same as example 1. Finally, the obtained astaxanthin oil contained 18.9 μg of astaxanthin, and the organic solvent was recovered; 0.64 g of complex protein peptide powder rich in calcium lactate and amino acids was obtained; and 1.97 g of crude chitin was obtained.

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Abstract

The invention relates to a method for extracting astaxanthin, protein, calcium lactate and chitin from shrimp heads through microbes. A supernatant fluid and a solid precipitate are respectively collected by centrifugation after fermentation treatment is performed on the shrimp head shells by utilizing streptococcus thermophilus under proper conditions. The solid precipitate is soaked in ethanol and then centrifugated, and the liquid part is evaporated for recovering an organic solvent so as to get the astaxanthin; the residual solid part is dried to get a chitin crude product; and the centrifugated supernatant fluid is subjected to spray-drying and concentration so as to get composite protein peptide powder which is rich in the calcium lactate and amino acids. The process has the advantages of reasonable process, no pollution, complete utilization of raw materials, high product quality and the like.

Description

technical field [0001] The invention relates to the technical field of extracting astaxanthin, protein, calcium lactate and chitin from shrimp heads by using microbial fermentation technology. Background technique [0002] Astaxanthin, the full name is 3,3'-dihydroxy-β-carotene-4,4'-ketone, which belongs to ketone carotenoids, is bright red, fat-soluble, and has high-efficiency anti-oxidation, anti-cancer, enhances Functions such as immunity and coloring. Due to the optical activity of the hydroxyl groups (-OH) at both ends, astaxanthin has three isomers: 3S-3'S, 3R-3'S, 3R-3'R (also known as left-handed, racemic, and right-handed). form. The latest pharmacological and physiological studies have found that astaxanthin has strong biological antioxidant properties and has been widely used in food, medicine, cosmetics and feed industries. A number of human and animal experiments have confirmed that natural astaxanthin is absolutely safe for humans and animals. [0003] At p...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12P21/06C12P3/00C12P19/14C12P19/04C07C403/24C12R1/46
Inventor 毛相朝林洪张婧高原松胡增淼李钰金
Owner OCEAN UNIV OF CHINA
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