Anti-plant pathogenic fungi composition and its preparation method

A plant pathogenic fungus and composition technology, applied in the field of pesticides, can solve problems such as sunken disease spots, affecting the quality of tobacco leaves, and pollution of human living environment, and achieve the effect of high inhibition rate and remarkable antibacterial effect

Inactive Publication Date: 2012-08-01
SHIHEZI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] According to reports, phytopathogenic fungi can cause yield reduction of food crops, rust spots on leaves or fruits of vegetable crops, and cause great harm to agricultural production
For example: Rhizoctonia solani is a soil pathogenic fungus with a wide range of hosts, which can cause diseases on various crops and vegetables such as rice, corn, cotton, sugar beet, potato, wax gourd and cucumber, resulting in rotten roots and stems or rotting leaves and other disease symptoms, for this, the currently commonly used pesticides are chemical pesticides such as tolclofos-methyl EC, tolclofos and agricultural antibiotics, but the results of use show that there are still many diseases; Fusarium oxysporum (Fusarium oxysporum) infects banana plants, known as Banana Panama disease, which causes wilting and death of the entir

Method used

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  • Anti-plant pathogenic fungi composition and its preparation method
  • Anti-plant pathogenic fungi composition and its preparation method
  • Anti-plant pathogenic fungi composition and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of Antifungal Composition Raw Materials

[0034] 2 kg of celery (Apium Graveolens L.) seeds were crushed, extracted 4 times with 10 times the volume of 95% ethanol, evaporated to remove the ethanol, the extract was shaken with water to form a suspension, extracted 4 times with petroleum ether to obtain 170 g of the extract.

Embodiment 2

[0035] Example 2 Component Analysis of Antifungal Composition Raw Materials

[0036] The composition analysis and determination of antifungal components were completed by gas chromatography-mass spectrometry (GC-MS). mm); (2) Analytical conditions and methods: the carrier gas is helium (99.999%), the flow rate is 1.0 ml / min, the injection volume is 1.0 microliters; the inlet temperature is 150 o C, keep for 2 min, and then 15 o C / min to 300 o C stabilized for 5 minutes; (3) Mass spectrometry conditions: ionization mode is EI source, ionization energy 70 electron volts, mass range (41-450) amu; (4) component analysis Replib, Mainlib spectral library comparison.

[0037] The peaks in the total ion chromatogram were scanned by mass spectrometry to obtain the mass spectrogram, searched and analyzed through the mass spectrogram library, and the relative percentage content of them in the fat-soluble components was obtained by using the peak area normalization method. The result...

Embodiment 3

[0041] Evaporate the solvent ethanol and extract with petroleum ether. The petroleum ether extract was chromatographed on a silica gel column, and eluted with a gradient of petroleum ether-ethyl acetate (100:0 – 0:100) solvent system; the specific low-polarity part was obtained, and the solvent was evaporated to dryness to obtain QCZ -28; GC-MS was used to analyze the active site (QCZ-28) obtained from the raw material, and the analysis results were as follows figure 1 and shown in Table 1.

[0042]

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Abstract

The invention belongs to the pesticide science field, and relates to an anti- plant pathogenic fungi compositionQCZ-28 and its preparation method; the composition is consist of three main components of 3-(3,4-dimethyl benzoyl)propionic acid, p-heptylphenol and p-(2-amino ethyl)with similar proportion, the main components is prepared by purifying a low polar part of a celery seeds ethanol extract under certain condition through chromatogram. According to the invention, the natural antifungal composition QCZ-28 extracted in vegetable seed celery seeds possesses high inhibition rate on Rhizoctonia solani, Fusarium oxysporum and Alternaria alternate, substantial effect on antibiosis function and no adverse influence on human body and environment.

Description

technical field [0001] The invention belongs to the field of pesticide science and relates to anti-plant fungal pesticides, in particular to an anti-phytopathogenic fungus composition (QCZ-28) and a preparation method thereof. Background technique [0002] According to reports, phytopathogenic fungi can cause yield reduction of food crops, rust spots on leaves or fruits of vegetable crops, and cause great harm to agricultural production. For example: Rhizoctonia solani is a soil pathogenic fungus with a wide range of hosts, which can cause diseases on various crops and vegetables such as rice, corn, cotton, sugar beet, potato, wax gourd and cucumber, resulting in rotten roots and stems or rotting leaves and other disease symptoms, in view of this, currently commonly used pesticides are chemical pesticides such as tolclofos-methyl EC, tolclofos and agricultural antibiotics, but the results of use show that there are still many diseases; Fusarium oxysporum (Fusarium oxysporum...

Claims

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Application Information

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IPC IPC(8): A01N45/02A01N37/42A01N33/10A01N31/08A01P3/00
Inventor 谢慧琴刘涛刘福光穆青
Owner SHIHEZI UNIVERSITY
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