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Method for separating and purifying DHA (docosahexaenoic acid) and saturated fatty acid from schizochytrium limacinum oil

A technology of unsaturated fatty acids and fatty acids, applied in the separation/purification of carboxylic acid compounds, fatty acid production, fatty acid preparation/refining, etc., can solve the problems of inability to achieve high purification, DHA, saturated fatty acid separation and purification research reports are few and other issues , to achieve considerable economic value, retention of biological activity and nutrition, and high product yield.

Active Publication Date: 2013-10-02
福建华尔康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, the research and large-scale production of DHA by Schizochytrium fermentation have been widely carried out in the world. At present, the research on Schizochytrium mainly focuses on the improvement and selection of fermentation production method and oil extraction process. The extracted Fat contains not only DHA, but also more saturated fatty acids (content reaches 30%-40%), which cannot achieve the purpose of high purification
At present, there are few research reports on the separation and purification of DHA and saturated fatty acids in Schizochytrium oil in my country

Method used

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  • Method for separating and purifying DHA (docosahexaenoic acid) and saturated fatty acid from schizochytrium limacinum oil
  • Method for separating and purifying DHA (docosahexaenoic acid) and saturated fatty acid from schizochytrium limacinum oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of free mixed fatty acids

[0028] Take 100g of Schizochytrium algae oil into a flask, add 400mL of prepared 4% NaOH-95% ethanol solution, fill with nitrogen protection, stir and reflux in a water bath at 55°C for 1.5h, cool to room temperature, add 8g of chloride sodium, stirred, and stood for 15 minutes, filtered to obtain filtrate and solid, discarded the filtrate, added water and stirred the solid until it was completely dissolved, adjusted the pH to 2.5 with 10% hydrochloric acid, then added 2 times the volume of n-hexane to extract and separate with a separatory funnel, and obtained The oil in the upper organic phase was washed with distilled water to neutrality, added anhydrous sodium sulfate for dehydration, and suction filtered. The filtrate was concentrated under reduced pressure at 40°C and a vacuum of 0.08 MPa, and n-hexane was recovered to obtain 87g of free mixed fatty acids.

[0029] (2) Urea inclusion treatment

[0030] Weigh 174g of ure...

Embodiment 2

[0039] (1) Preparation of free mixed fatty acids

[0040] Put 100g of Schizochytrium oil into a flask, add 500mL of prepared 4% NaOH-95% ethanol solution, fill with nitrogen protection, stir and reflux in a water bath at 50°C for 1h, after cooling to room temperature, add 10g of sodium chloride , stirred and stood still for 15 minutes, filtered to obtain the filtrate and solid, discarded the filtrate, added water and stirred the solid until it was completely dissolved, adjusted the pH to 2.6 with 10% hydrochloric acid, then added 2 times the volume of n-hexane to extract and separate with a separatory funnel, and took the upper layer The oil in the organic phase was washed with distilled water until neutral, dehydrated with anhydrous sodium sulfate, and suction filtered. The filtrate was concentrated under reduced pressure at 40°C and a vacuum of 0.08 MPa, and n-hexane was recovered to obtain 85 g of free mixed fatty acids.

[0041] (2) Urea inclusion treatment

[0042]Weigh ...

Embodiment 3

[0050] Schizochytrium oil raw material of the present invention and embodiment 1,2 gained product are carried out content analysis with gas chromatography, wherein the content of DHA, DPA, palmitic acid is shown in Table 1:

[0051] Table 1 Analysis of fatty acid content

[0052]

[0053] 1. Detection method of fatty acid composition:

[0054] The sample was first treated with the "boron trifluoride method" in GB / T 17376-2008 "Preparation of Fatty Acid Methyl Esters of Animal and Vegetable Oils", and then the content was analyzed by gas chromatography.

[0055] 2. Calculation formula:

[0056] Polyunsaturated fatty acid yield =

[0057] Saturated fatty acid yield =

[0058] 3. Conclusion:

[0059] (1) Polyunsaturated fatty acids and saturated fatty acids in Schizochytrium oil raw materials were effectively separated.

[0060] (2) The product has high purity. The content of DHA increased from 42.03% to 73%, the content of DPA increased from 12.11% to 20%, and the c...

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Abstract

The invention discloses a method for separating and purifying DHA (docosahexaenoic acid) and saturated fatty acid from schizochytrium limacinum oil. The method includes: firstly, saponifying, salting out and acidizing the schizochytrium limacinum oil under protection of nitrogen so as to obtain free mixed fatty acid, and separating the fatty acid different in degree of saturation by urea adduction fractionation so as to obtain filtrate and solids after filtration; concentrating and extracting the filtrate so as to obtain polyunsaturated fatty acid rich in DHA and DPA (docosapentenoic acid); and allowing the solids to leach by means of acidolysis, extracting the saturated fatty acid (mainly comprising palmic acid) and recovering urea, wherein the urea is recyclable. The method is performed at a low temperature, oxidization of the unsaturated fatty acid is avoided, biological activity and nutrition of the unsaturated fatty acid are kept intact, and the problem of residual solvent is avoided. Products are high in purity, and the obtained polyunsaturated fatty acid mainly comprises the DHA and the DPA and hardly comprises EPA (eicosapentaenoic acid), the content of the DHA and the DPA is higher than 93%, and the content of the palmic acid of the saturated fatty acid is higher than 82%.

Description

technical field [0001] The invention relates to a separation and purification technology of oil, in particular to a preparation method for separation and purification of DHA and saturated fatty acid in Schizochytrium oil. Background technique [0002] DHA is docosahexaenoic acid, commonly known as "brain gold". It is an important omega-3 polyunsaturated fatty acid and an important component of cephalin. Cancer, vision protection, promotion of intellectual development, prevention and treatment of senile dementia and many other important physiological functions. Insufficient DHA will lead to brain development disorders in infants, mental retardation in adolescents, and premature degeneration of brain nerves in middle-aged and elderly people. DPA is docosapentaenoic acid, which belongs to omega-3 polyunsaturated fatty acid. It is a long-chain unsaturated fatty acid only found in human colostrum. It is also the main component of human brain tissue and nerve cells; and DHA act...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C51/42C11C1/02C11C1/08C07C51/02C07C57/03
Inventor 张河溪魏巧容郭震宇吴松刚黄建忠林金科郭耀荣
Owner 福建华尔康生物科技有限公司