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Method for culturing oil-producing microalgae with high density and high lipid production rate

An oil-producing microalgae, high-density technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of error-prone, troublesome nutrient supply, etc., achieve simple and feasible operation, reduce production costs, improve The effect of algal cell concentration

Active Publication Date: 2013-04-17
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
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Problems solved by technology

However, these techniques and methods still have limitations, and controlling the intricate nutrient supply is cumbersome and error-prone

Method used

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  • Method for culturing oil-producing microalgae with high density and high lipid production rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Glucose is selected as the carbon source, potassium nitrate is used as the nitrogen source, and a high-concentration sterile additive solution is prepared.

[0022] (2) Get oleaginous microalga Chlorella (Chlorella kessleri) and culture in dark heterotroph in liquid medium, medium is Kuhl medium, formula: 10g / L glucose, 1g / L potassium nitrate, 89mg / L twelve Disodium hydrogen phosphate water, 621mg / L sodium dihydrogen phosphate dihydrate, 246mg / L magnesium sulfate heptahydrate, 9.3mg / L EDTA, 6.9mg / L ferrous sulfate heptahydrate, 14.7mg / L calcium chloride dihydrate, 0.29mg / L zinc sulfate heptahydrate, 0.17mg / L manganese sulfate monohydrate, 0.06mg / L boric acid, 0.002mg / L copper sulfate pentahydrate, 0.012mg / L ammonium molybdate tetrahydrate, pH 6.5. The algae were inoculated into a 250ml Erlenmeyer flask containing 100ml of sterile Kuhl medium at a 10% (volume ratio) inoculum, and cultured in a shaker at a temperature of 25° C. in continuous darkness with a rotating s...

Embodiment 2

[0025] (1) Glucose is selected as the carbon source, potassium nitrate is used as the nitrogen source, and a high-concentration sterile additive solution is prepared.

[0026] (2) Get oil-producing microalgae Chlorella (Chlorella kessleri) in liquid medium for photoheterotrophic culture, medium is Kuhl medium, formula: 10g / L glucose, 1g / L potassium nitrate, 89mg / L twelve Disodium hydrogen phosphate water, 621mg / L sodium dihydrogen phosphate dihydrate, 246mg / L magnesium sulfate heptahydrate, 9.3mg / L EDTA, 6.9mg / L ferrous sulfate heptahydrate, 14.7mg / L calcium chloride dihydrate, 0.29mg / L zinc sulfate heptahydrate, 0.17mg / L manganese sulfate monohydrate, 0.06mg / L boric acid, 0.002mg / L copper sulfate pentahydrate, 0.012mg / L ammonium molybdate tetrahydrate, pH 6.5. The algal species were inoculated into a 250ml Erlenmeyer flask containing 100ml of sterile Kuhl medium at a temperature of 25°C, a rotational speed of 150rpm, and a light intensity of 100μmol m -2 the s -1 Shake cult...

Embodiment 3

[0029] (1) Select sucrose as the carbon source and urea as the nitrogen source, and prepare a high-concentration sterile additive solution.

[0030] (2) Take oil-producing microalga Chlorella (Chlorella zofingiensis) and culture it photoheterotrophically in liquid medium, the medium is Kuhl medium, formula: 10g / L sucrose, 0.3g / L urea, 89mg / L twelve Disodium hydrogen phosphate water, 621mg / L sodium dihydrogen phosphate dihydrate, 246mg / L magnesium sulfate heptahydrate, 9.3mg / L EDTA, 6.9mg / L ferrous sulfate heptahydrate, 14.7mg / L calcium chloride dihydrate, 0.29mg / L zinc sulfate heptahydrate, 0.17mg / L manganese sulfate monohydrate, 0.06mg / L boric acid, 0.002mg / L copper sulfate pentahydrate, 0.012mg / L ammonium molybdate tetrahydrate, pH 6.5. The algal species were inoculated into a 250ml Erlenmeyer flask containing 100ml of sterile Kuhl medium at a temperature of 25°C, a rotational speed of 150rpm, and a light intensity of 100μmol m -2 the s -1 Culture in a shaker with continuous...

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Abstract

The invention belongs to the technical field of unicellular oil and biodiesel, and in particular relates to a method for synchronously improving biomass and lipid production rate of the oil-producing microalgae. The method comprises the following steps of: culturing the oil-producing microalgae in a logarithmic phase into a liquid culture medium of sterile addictive solution into which carbon sources and / or nitrogen sources are added; and culturing a mixed solution for 6-40 days in a swing bed which is continuously irradiated at the temperature of 18-35 DEG C, the rotation speed of 100-200rpm and the illumination intensity of 0-150 mu mol m-2s<-1>, wherein the sterile addictive solution is added into the liquid culture medium of the addictive solution into which carbon sources and / or nitrogen sources are added during culture. By the method, the growth process and the lipid production process of the oil-producing microalgae can be technically integrated through an alternating and circular control mode, so that the concentration of algae cells can be improved, the lipid can be effectively produced at the same time without replacement of culture medium compositions, batch supply of full-nutrition nourishment materials and real-time calculation of acceleration rate of nourishment material flow, and therefore, the method is easy to operate, and greatly reduces the production cost.

Description

technical field [0001] The invention belongs to the technical field of single-cell oil and biodiesel production, in particular to a method for synchronously increasing the biomass and lipid production of oil-producing microalgae. Background technique [0002] Oil-producing microalgae are rich in resources, can grow under a variety of culture conditions, have high photosynthetic efficiency, high biomass, fast growth and reproduction, short growth cycle, and strong ability to synthesize oil by themselves. and one of the best raw materials for biodiesel. Microalgae oil can produce biodiesel through transesterification with low-carbon alcohols, and the use of microalgae to produce biodiesel has great potential to alleviate the energy shortage and environmental pollution faced by human beings, and at the same time reduce the use of fossil energy in production and life. Relying on and ensuring national energy security has far-reaching significance. Microalgae oil can be used to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 王艳秦松
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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