Direct-vat-set (DVS) mildewed bean dreg starter as well as preparation method and application thereof

The technology of moldy bean dregs and starter, which is applied in the industrial production field of moldy bean dregs, can solve the problems of food safety, convenience and unstable quality of periodic products, so as to shorten the maturity period of products, maintain stability and simplify product production. The effect of craftsmanship

Inactive Publication Date: 2013-05-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the phenomenon of unstable product quality caused by the defects in edible safety, convenience and production cycle of the current production process of mold

Method used

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  • Direct-vat-set (DVS) mildewed bean dreg starter as well as preparation method and application thereof
  • Direct-vat-set (DVS) mildewed bean dreg starter as well as preparation method and application thereof
  • Direct-vat-set (DVS) mildewed bean dreg starter as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0040] Example 1

[0041] The preparation of direct-throwing moldy bean dregs starter is realized by the following steps:

[0042] 1. Preparation of Yagi radiomucora agent

[0043] a. Preparation of culture medium

[0044] Bran culture medium: After mixing 100 g of bran with 150 mL of water, add 1% glucose, 0.2% calcium chloride, 0.05% copper sulfate, 0.05% ammonium sulfate by weight, mix well, and then dispense into 250 mL conical flasks 50g per bottle, the sterilization time is 20min under the temperature condition of 121℃.

[0045] Potato dextrose agar medium PDA: 200.0 g of potatoes (chopped, boiled in boiling water for 30 min, filtered to get the clear liquid), 20.0 g of glucose, 20.0 g of agar, dilute to 1000 mL, and sterilize at 121 °C for 15 min.

[0046] b. Activation of slanted bacteria

[0047] Under sterile conditions, inoculate Mucor radiatae on the PDA slant medium, place it in an incubator, and cultivate at 28°C for 3 days, which is a solid test tube strain ...

Example Embodiment

[0082] Example 2

[0083] 1. Preparation of moldy bean dregs products

[0084] 1. The steps of producing moldy bean dregs by direct-throwing moldy bean dregs starter are as follows:

[0085] (1) Fresh wet bean dregs are washed with water and pressed to a water content of 75%;

[0086] (2) Put the pressed bean dregs into the steaming cabinet in layers, and cook for 20min under normal pressure;

[0087] (3) After the steaming is completed, cool down to room temperature, activate the direct-injection moldy bean dregs starter with physiological saline for 30 minutes at room temperature, and inoculate 0.1% direct-injection moldy bean dregs starter into the cooked bean dregs, stir evenly, and take it out. 30g bean dregs were pressed into balls and fermented in an artificial climate box for 3 days, the temperature was set at 28°C, and the relative humidity was controlled at 90%.

[0088] 2. The preparation of the moldy bean dregs koji The moldy bean dregs process is as follows: th...

Example Embodiment

[0106] Example 3

[0107] The steps that direct-throwing moldy bean dregs starter is applied to produce moldy bean dregs are as follows:

[0108] (1) Fresh wet bean dregs are washed with water and pressed to a water content of 75%;

[0109] (2) Put the pressed bean dregs into the steaming cabinet in layers, and cook for 15 minutes under normal pressure;

[0110] (3) After the steaming is completed, cool down to room temperature, activate the direct-injection moldy bean dregs starter with physiological saline for 30 minutes at room temperature, inoculate 0.02% of the direct-injection moldy dregs starter into the cooked bean dregs, stir evenly, and take 30g bean dregs were pressed into a cake shape and fermented in an artificial climate box for 6 days, the temperature was set at 28°C, and the relative humidity was controlled at 95%.

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Abstract

The invention discloses a direct-vat-set (DVS) mildewed bean dreg starter as well as a preparation method and an application of the DVS mildewed bean dreg starter. The invention belongs to the field of bean dreg processing. According to the invention, the preparation method comprises the following steps of: performing solid culture, spore elution filtration and centrifugal concentration on actinomucor elegans to obtain centrifuged precipitates; adding a certain proportion of a freeze-drying protective additive into the centrifuged precipitates and performing vacuum freeze drying to obtain an actinomucor elegans inoculant; inoculating zymomonas mobilis into a YPD (Yeast Peptone Dextrose) liquid culture medium to carry out shake cultivation; performing centrifugal concentration on the cultured solution to obtain centrifuged precipitates; adding a certain proportion of the freeze-drying protective additive into the centrifuged precipitates and performing vacuum freeze drying to obtain a zymomonas mobilis inoculant; and then compounding the actinomucor elegans inoculant with the zymomonas mobilis inoculant to obtain the DVS mildewed bean dreg starter. After inoculated into sterilized bean dregs, the inoculant can be used for fermenting mildewed bean dregs. The DVS mildewed bean dreg starter is beneficial to large-scale and standardized production of the mildewed bean dregs.

Description

technical field [0001] The present invention relates to a preparation method of a direct-throwing moldy bean dregs starter, in particular to the enrichment, concentration and drying of Mucor radiata and Zymomonas mobilis, and the compounding of the obtained bacterial agent before application It is used in the industrial production of moldy bean dregs. Background technique [0002] Okara contains most of the crude fiber (composed of fiber, semi-fiber, and lignin), rich in protein (24.5-37.5g / 100g solid matter), and dietary fiber (14.5-55.4g / 100g solid matter), as well as isoflavones (0.14g / 100g solid matter), so okara has high nutritional value. The dietary fiber contained in soybeans has a variety of health effects, and is called the "seventh nutrient" by nutritionists after protein, fat, carbohydrates, water, minerals and vitamins. In addition, bean dregs also contain soybean protein, calcium, phosphorus, vitamins and so on. Therefore bean dregs is a precious resource th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A23L1/20C12R1/785C12R1/01A23L11/00
Inventor 许喜林石美芬樊慧明
Owner SOUTH CHINA UNIV OF TECH
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