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Direct-vat-set (DVS) mildewed bean dreg starter as well as preparation method and application thereof

The technology of moldy bean dregs and starter, which is applied in the industrial production field of moldy bean dregs, can solve the problems of food safety, convenience and unstable quality of periodic products, so as to shorten the maturity period of products, maintain stability and simplify product production. The effect of craftsmanship

Inactive Publication Date: 2013-05-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the phenomenon of unstable product quality caused by the defects in edible safety, convenience and production cycle of the current production process of moldy bean dregs, and provides a method for preparing a direct-throwing moldy bean dregs starter, by improving the process flow , significantly improving production efficiency and product stability

Method used

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  • Direct-vat-set (DVS) mildewed bean dreg starter as well as preparation method and application thereof
  • Direct-vat-set (DVS) mildewed bean dreg starter as well as preparation method and application thereof
  • Direct-vat-set (DVS) mildewed bean dreg starter as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation of direct-throwing moldy bean dregs starter is realized by the following steps:

[0042] 1. Preparation of Yazhi radiomucor fungus

[0043] a. Preparation of culture medium

[0044] Bran culture medium: After mixing 100g of bran and 150mL of water, add 1% glucose, 0.2% calcium chloride, 0.05% copper sulfate, 0.05% ammonium sulfate by weight, mix well and distribute in 250mL triangular flask 50g per bottle, the sterilization time is 20min at 121°C.

[0045] Potato Dextrose Agar Medium PDA: Potato 200.0g (chopped, boiled for 30min, filtered to get the clear liquid), glucose 20.0g, agar 20.0g, dilute to 1000mL, sterilize at 121°C for 15min.

[0046] b. Activation of slant strains

[0047] Under aseptic conditions, inoculate Actinococcus jazhi on the PDA slant medium, place it in an incubator, and cultivate it at 28°C for 3 days, and then it becomes a solid test tube strain, which is ready for use.

[0048] c. Preparation of Mucor actinosa spore suspensi...

Embodiment 2

[0083] 1. Preparation of moldy bean dregs

[0084] 1. The steps of producing moldy bean dregs with direct-injection moldy bean dregs starter are as follows:

[0085] (1) Fresh wet bean dregs are washed and squeezed to a water content of 75%;

[0086] (2) Put the pressed bean dregs into the steaming cabinet layer by layer, and cook for 20 minutes under normal pressure;

[0087] (3) Cool down to room temperature after steaming, activate the direct-injection moldy bean dregs starter with normal saline for 30 minutes at room temperature, inoculate 0.1% direct-injection moldy bean dregs starter into the cooked bean dregs, stir well and take out 30g of bean dregs were pressed into balls and fermented in an artificial climate box for 3 days, the temperature was set at 28°C, and the relative humidity was controlled at 90%.

[0088] 2. Preparation of moldy bean dregs koji The moldy bean dregs process is as follows: the water content of the bean dregs is 60%, the steaming time is 30 m...

Embodiment 3

[0107] The steps of direct-throwing moldy bean dregs starter applied to the production of moldy bean dregs are as follows:

[0108] (1) Fresh wet bean dregs are washed and squeezed to a water content of 75%;

[0109] (2) Put the pressed bean dregs into the steaming cabinet layer by layer, and cook for 15 minutes under normal pressure;

[0110] (3) Cool down to room temperature after steaming, activate the direct-injection moldy bean dregs starter with normal saline for 30 minutes at room temperature, inoculate 0.02% direct-injection moldy bean dregs starter into the cooked bean dregs, stir well and take out 30g of bean dregs were pressed into a cake shape, and fermented in an artificial climate box for 6 days, the temperature was set at 28°C, and the relative humidity was controlled at 95%.

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Abstract

The invention discloses a direct-vat-set (DVS) mildewed bean dreg starter as well as a preparation method and an application of the DVS mildewed bean dreg starter. The invention belongs to the field of bean dreg processing. According to the invention, the preparation method comprises the following steps of: performing solid culture, spore elution filtration and centrifugal concentration on actinomucor elegans to obtain centrifuged precipitates; adding a certain proportion of a freeze-drying protective additive into the centrifuged precipitates and performing vacuum freeze drying to obtain an actinomucor elegans inoculant; inoculating zymomonas mobilis into a YPD (Yeast Peptone Dextrose) liquid culture medium to carry out shake cultivation; performing centrifugal concentration on the cultured solution to obtain centrifuged precipitates; adding a certain proportion of the freeze-drying protective additive into the centrifuged precipitates and performing vacuum freeze drying to obtain a zymomonas mobilis inoculant; and then compounding the actinomucor elegans inoculant with the zymomonas mobilis inoculant to obtain the DVS mildewed bean dreg starter. After inoculated into sterilized bean dregs, the inoculant can be used for fermenting mildewed bean dregs. The DVS mildewed bean dreg starter is beneficial to large-scale and standardized production of the mildewed bean dregs.

Description

technical field [0001] The present invention relates to a preparation method of a direct-throwing moldy bean dregs starter, in particular to the enrichment, concentration and drying of Mucor radiata and Zymomonas mobilis, and the compounding of the obtained bacterial agent before application It is used in the industrial production of moldy bean dregs. Background technique [0002] Okara contains most of the crude fiber (composed of fiber, semi-fiber, and lignin), rich in protein (24.5-37.5g / 100g solid matter), and dietary fiber (14.5-55.4g / 100g solid matter), as well as isoflavones (0.14g / 100g solid matter), so okara has high nutritional value. The dietary fiber contained in soybeans has a variety of health effects, and is called the "seventh nutrient" by nutritionists after protein, fat, carbohydrates, water, minerals and vitamins. In addition, bean dregs also contain soybean protein, calcium, phosphorus, vitamins and so on. Therefore bean dregs is a precious resource th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A23L1/20C12R1/785C12R1/01A23L11/00
Inventor 许喜林石美芬樊慧明
Owner SOUTH CHINA UNIV OF TECH
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