Application of k-carrageenan oligosaccharides capable of adjusting activated states of gitter cell

A technology of microglia and carrageenan oligosaccharides, applied in the field of κ-carrageenan oligosaccharides in medicine and health food, can solve the problems of aggravating nerve cell damage and achieve the effect of improving the body's immunity

Inactive Publication Date: 2013-06-05
WEIHAI KANGBOER BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the pathogenesis of common central nervous system diseases has not been fully studied, but microglia play a vital role in the course of the disease. On the one hand, microgli

Method used

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  • Application of k-carrageenan oligosaccharides capable of adjusting activated states of gitter cell
  • Application of k-carrageenan oligosaccharides capable of adjusting activated states of gitter cell
  • Application of k-carrageenan oligosaccharides capable of adjusting activated states of gitter cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Effect of κ-carrageenan oligosaccharide (KCOS) on the proliferation of microglial cells

[0024] 1.1 Inverted microscope observation results of the effect of κ-carrageenan oligosaccharides on the proliferation of microglial cells

[0025] Cell culture conditions: IMDM (sigma, USA) medium containing 10% calf serum, 100 μg / ml streptomycin and 100IU / ml penicillin, 5% CO 2 , 37°C, subculture every two days. The day before the experiment, the N9 medium was changed. Wash 3 times with PBS, digest with 0.125% trypsin, pipette, and make cell density 5×10 with IMDM medium containing 10% fetal bovine serum 4 cells / mL of cell suspension. In a 96-well culture plate, add 200 μl of cell suspension to each well. After culturing for 12 hours, carefully absorb the culture medium and wash it with PBS. In the experimental group, add different concentrations of KCOS and DKCOS (the desulfurization group of κ-carrageenan oligosaccharide) to each well. derivatives), so that the ...

Embodiment 2

[0030] Example 2: Effects of κ-carrageenan oligosaccharides on cytokines secreted by microglia

[0031] 2.1 Griess method test results of the effect of κ-carrageenan oligosaccharides on the release of NO from microglia

[0032] NO is easily oxidized to NO in the body or in aqueous solution 2- , under acidic conditions, NO 2- The diazonium reaction occurs with the diazonium salt sulfonamide, and a diazo compound is generated, and the latter further undergoes a coupling reaction with naphthyl vinyl diamine, and the concentration of the product generated by the reaction is the same as that of NO 2- The concentration has a linear relationship, and there is a maximum absorption peak at 540nm. Determination of NO / NO by Griess method 2- Take the cell culture supernatant and add an equal volume of Griess reagent (1.0% anhydrous sulfanilic acid, 0.10% N-1-naphthylethylenediamine hydrochloride, 2.5% phosphoric acid), and measure the absorbance at 540 nm.

[0033] Results: NO is an i...

Embodiment 3

[0037] Example 3: The way κ-carrageenan oligosaccharides act on microglia

[0038] 3.1 Laser confocal detection of the effect of FITC-labeled κ-carrageenan oligosaccharides on microglia

[0039] The pure product of fluorescein isothiocyanate (FITC) is yellow or orange-yellow crystalline powder, easily soluble in water and alcohol solvents. The molecular weight is 389.4, the maximum absorption wavelength is 490-495nm, and the maximum emission wavelength is 520-530nm. It presents bright yellow-green fluorescence and is currently the most widely used fluorescein. Since κ-carrageenan oligosaccharide itself has a negative charge, it can be combined with FITC through charge interaction, and can be used to indicate the interaction between oligosaccharides and cells under a fluorescence microscope.

[0040] 3.1.1 FITC-labeled κ-carrageenan oligosaccharides:

[0041] Mix κ-carrageenan oligosaccharide with equal mass of FITC, dissolve in a certain amount of distilled water, stir at ro...

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Abstract

The invention discloses application of k-carrageenan oligosaccharides capable of adjusting activated states of a gitter cell. The k-carrageenan oligosaccharides is used for the gitter cell in a quiescent condition, can promote cell multiplication and improve cell viability, and can further effectively prevent lipopolysaccharide from excessively activating activity of the gitter cell, and reduce damage of peripheral nerve cells because of excessive multiplication of the gitter cell and release of inflammatory factors. The k-carrageenan oligosaccharides can be used for preparing medicine for treatment of nervous system disease and prevention of the nervous system disease which are mediated by the gitter cell. The k-carrageenan oligosaccharides can also be used for improving immunity of organisms by promoting multiplication of immune cells such as the gitter cell. As an agent for treatment of the nervous system disease and an agent for prevention of the nervous system disease, the application of the k-carrageenan oligosaccharides capable of adjusting the activated states of the gitter cell can be added to health food for preventing the nervous system disease and improving the immunity of the organisms and drugs for preventing the nervous system disease and improving the immunity of the organisms, and can also be added to any liquid food and solid food.

Description

technical field [0001] The invention relates to the application of κ-carrageenan oligosaccharide capable of regulating the activation state of microglial cells in the field of medicine and health food, especially in the fields of anti-autoimmune system diseases, anti-ischemia-reperfusion injury, anti-infection and anti-chronic inflammation reactions, etc., and as a health food in improving the immunity of the body. Background technique [0002] Carrageenan is a polysaccharide containing sulfate groups extracted from red algae. Kappa-carrageenan is composed of repeating units of 3,6-endether-α-D-galactose and 4-sulfate-β-D-galactose. Studies have found that sulfated polysaccharides have relatively unique biological activities. Compared with polysaccharides, carrageenan oligosaccharides have a more uniform structure, relatively smaller molecular weight, are easy to dissolve, are easier to be used by the body, and are easier to separate and purify, which is convenient for anal...

Claims

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Application Information

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IPC IPC(8): A61K31/737A61P25/00A61P25/28A61P25/16A61P25/08A61P9/10A61P37/04A61P29/00A61P31/00A23L1/29A23L33/00
Inventor 吴海歌姚子昂于国友于丽萍
Owner WEIHAI KANGBOER BIOLOGICAL PHARMA
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