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Method for cleaning and producing lincomycin

A technology of lincomycin and production methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve environmental pollution and other problems, and achieve the effect of reducing production costs

Inactive Publication Date: 2013-06-26
YAOPHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem of environmental pollution caused by wastewater produced by lincomycin fermentation, the invention provides a new method for clean production of lincomycin

Method used

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  • Method for cleaning and producing lincomycin
  • Method for cleaning and producing lincomycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: preparation of lincomycin seed solution

[0022] The lincomycin-producing bacterium is Streptomyces lincolnensis lincomycin-producing bacterium ( Streptomyces lincolnensis ) CPCC-200038 strain

[0023] Slant medium: 2% starch, 0.05% sodium chloride, 0.5% potassium nitrate, 0.1% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, 0.01‰ ferrous sulfate, 1.8% agar, the balance is water, and the pH is natural.

[0024] Seed medium: 1.5% starch, 3% glucose, 1.5% bean cake powder, 2.5% corn steep liquor, 0.2% sodium nitrate, 0.2% ammonium sulfate, 0.05% potassium dihydrogen phosphate, 0.5% calcium carbonate, 0.05% soybean oil, foam enemy 0.01%, the balance is water, pH value: 7.6~7.8 (before elimination).

[0025] Mother slant seed preparation: in a sterile room, take 1 / 5 shovel of sandy soil spores and inoculate them on the sterile and normal test tube slant after one day of cultivation under aseptic conditions, spread evenly, and cultivate at 29°C with ...

Embodiment 2

[0033] Embodiment 2: the preparation of lincomycin fermented liquid

[0034] Initial fermentation medium: starch saccharification solution 6.5%, soybean cake powder 2%, corn steep liquor 1.5%, ammonium sulfate 0.38%, calcium carbonate 0.8%, sodium chloride 0.68%, potassium dihydrogen phosphate 0.03%, the balance is water, pH6.5~8.0.

[0035] Feed medium 1: soybean cake powder 10%, corn steep liquor 18%, calcium carbonate 0.8%, soybean oil 0.05Kg / m 3 , bubble enemy 0.01 Kg / m 3 , the balance is water, and the pH is natural.

[0036] Feed medium 2: soybean cake powder 4%, corn steep liquor 6%, sodium nitrate 2%, ammonium sulfate 1%, potassium dihydrogen phosphate 0.05%, calcium carbonate 0.5%, soybean oil 0.05Kg / m 3 , bubble enemy 0.05 Kg / m 3 , the balance is water, and the pH is natural.

[0037] Fermentation conditions:

[0038] The fermentation process is carried out in a 50L fermenter, the initial liquid volume is 50%, the inoculum volume is 6%, the stirring speed is 30...

Embodiment 3

[0044] Embodiment 3: the product preparation of lincomycin

[0045] About 40 L of the fermented liquid obtained in Example 2 was adjusted to 3.0 with oxalic acid, and the acidified liquid was filtered with a plate and frame to obtain 22 L of filtered clear liquid. The filtered clear liquid was adjusted to pH 10.5 with 20% sodium hydroxide solution. Re-filter the alkaline filtrate to ensure the clarification of the alkali solution, add 2 / 5 volume of mixed alcohol, start stirring for 20-30min, and let it stand for stratification. The upper organic phase is subjected to unit operations such as vacuum concentration, crystallization, decolorization, acetone crystallization, and drying to obtain the lincomycin product.

[0046]

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Abstract

The invention provides a novel method for cleaning and producing lincomycin. The method is characterized in that a lincomycin producing strain is adopted and used for fermenting at a ventilation condition; the obtained fermentation liquor is acidified and then filtered; the filtered clear liquid is alkalinized and then extracted by mixed alcohol; the extract liquid is centrifuged to separate, wherein the upper organic phase is used for extracting the lincomycin, and the lower aqueous phase is the producing wastewater which can be used as one of the components of fermentation mediums of the next batch after being subjected to component analysis, thus realizing the recycle of the producing wastewater. According to the method for cleaning and producing the lincomycin, the effective components in the producing wastewater are fully utilized, the purpose of turning wastes into wealth is realized, so that the waste of the resource, as well as the pollution to the environment, are reduced; the production cost is decreased, and the pressure for environmental protection due to the drainage of the producing waste is relieved at the same time.

Description

technical field [0001] The invention relates to a production method of biological raw materials, in particular to a new method for producing lincomycin through microbial fermentation. Background technique [0002] Lincomycin, also known as lincomycin in industry, is a lincosamide antibiotic isolated by Mason from Streptomyces cultured species in 1962, and its components include amino acids and sugars. Lincomycin has a good inhibitory effect on Gram-positive bacteria, it has no cross-resistance and its own toxicity is low, so it is widely used in diseases caused by Gram-positive bacteria. Negative bacteria also have a certain inhibitory effect. Lincomycin is widely distributed in the body and can enter bone marrow and bone tissue. It is widely used clinically for the treatment of diseases such as respiratory system, osteomyelitis and soft tissue infection, especially for patients who are allergic to penicillin and erythromycin. In addition, lincomycin is also used as a sta...

Claims

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Application Information

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IPC IPC(8): C12P19/64C12R1/565
Inventor 周浩然袁继安何刚
Owner YAOPHARMA CO LTD
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