Application of general inula helenium sesquiterpene lactone in preparation of medicine for treating rheumatoid arthritis

A technology of sesquiterpene lactone and inulin lactone, applied in the field of medicine, can solve problems such as excessive secretion of IL-17, and achieve the effects of simple preparation method, remarkable curative effect and convenient source of raw materials

Active Publication Date: 2015-07-15
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2. Bedard PA, Golds EE: Cytokine-induced expression of mRNAs for chemotactic factors in human synovial cells and fibroblasts. J Cell Physiol 1993, 154: 433-441) Increased IL-15 in rheumatoid arthritis models can be induced IL-17 exerts its pro-inflammatory cytokine properties, and IL-15 may lead to excessive secretion of IL-17 in joints through cyclosporin A and steroid-sensitive pathways

Method used

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  • Application of general inula helenium sesquiterpene lactone in preparation of medicine for treating rheumatoid arthritis
  • Application of general inula helenium sesquiterpene lactone in preparation of medicine for treating rheumatoid arthritis
  • Application of general inula helenium sesquiterpene lactone in preparation of medicine for treating rheumatoid arthritis

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Embodiment 1

[0024]Example 1 Preparation of total sesquiterpene lactones of inulin and inulin and different inulin

[0025] (1) 10kg of Tumuxiang medicinal material (commercially available, place of origin: Anguo, Hebei) was pulverized, then refluxed twice with 80L 60% ethanol in a heated reflux extraction tank (80L each time), 1 hour each time, combined the extracts, 85 Concentrate under reduced pressure at ℃-90℃ to 8L, which is equivalent to 0.8g of medicinal materials per ML, add 16L of water to dilute and add to D101 macroporous adsorption resin column, elute sugar with 100L of deionized water, and then use 100L of 40% ethanol Carry out elution, then carry out elution with 100L70% ethanol, collect this part, 85 ℃ of normal pressure dryings for 20 hours, obtain the total sesquiterpene lactones of inulin 265g, through HPLC method, with Agilent Zorbax C18 column (4.6*250mm , 5μ) for analysis, the mobile phase is acetonitrile-0.05% formic acid solution (55:45), the detection wavelength is ...

Embodiment 2

[0027] Embodiment 2 animal pharmacodynamics test

[0028] 1. Experimental materials

[0029] Drugs and Reagents

[0030] Chicken type II collagen (CⅡ): Chondrex, Redmond, WA98052, USA; Incomplete Freund's Adjuvant (IFA) Difco Laboratories, Detroit, MI; Phosphate buffered saline (PBS) preparation: Dissolve 8gNaCl in 800ml distilled water, 0.2 g KCl, 1.44 g Na 2 HPO 4 and 0.24g KH 2 PO 4 . Use hydrochloric acid to adjust the pH to 7.4, add water to make up to 1L, and use after filter sterilization; DMSO dimethyl sulfoxide (Dimethyl sulfoxide, DMSO): a product of Sigma Company; 1640 medium: Jinuo Biomedical Technology Co., Ltd.; small Bovine serum: Minhai Biological Co., Ltd.; 96-well flat bottom plate, CORNING; 70 μm cell grinding filter, BD Falcon; 15ml, 50ml centrifuge tubes, BD Falcon; 0.22μm filter, Millipore; 1ml screw-top syringe, BD ; Braun Tee, German Braun company; methotrexate tablets: Shanghai Pharmaceutical (Group) Co., Ltd. Xinyi Pharmaceutical General Factor...

Embodiment 3

[0065] Tablets: Inulin Total Sesquiterpene Lactones 30g

[0066] Lactose 190g

[0067] Cornstarch 75g

[0068] Magnesium Stearate 5g

[0069] Preparation method: mix the total sesquiterpene lactones of inulin, lactose and starch, moisten evenly with water, sieve the wet mixture and dry it, then sieve it, add magnesium stearate, and press the mixture into tablets, each tablet The weight is 300mg, and the total sesquiterpene lactone raw material containing inulin is 30mg.

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Abstract

The invention provides application of general inula helenium sesquiterpene lactone in preparation of a medicine for treating rheumatoid arthritis. The general inula helenium sesquiterpene lactone contains alantolactone and isoalantolactone; and the sum of the contents of the alantolactone and the isoalantolactone accounts for 60 to 90%. The results of animal pharmacodynamic tests show that the general inula helenium sesquiterpene lactone, the alantolactone and the isoalantolactone all can inhibit the clinical onset of CIA (Collagen-induced Arthritis), obviously inhibit the morbidity of CIA, obviously reduce the level of IFN(Interferon)-gamma, and remarkably inhibit the generation of IL(Interleukin)-17. Therefore, the general inula helenium sesquiterpene lactone, the alantolactone and the isoalantolactone all can be used for preparing the medicine for treating rheumatoid arthritis. The medicine is composed of the general inula helenium sesquiterpene lactone serving as an active ingredient and a pharmaceutic adjuvant and can be in form of a troche, a capsule, a soft capsule, a dropping pill, granules, an injection, a powder-injection or an aerosol and the like. The medicine provided by the invention is a new medicine for treating rheumatoid arthritis, which has remarkable curative effect and little side reaction; the raw materials are conveniently obtained, the preparation method is simple, and the medicine has relatively high clinical application value.

Description

technical field [0001] The invention relates to medicines, in particular to the application of inulin total sesquiterpene lactones prepared from the traditional Chinese medicine Inula inula genus inulin in the preparation of medicines for treating rheumatoid arthritis. Background technique [0002] Rheumatoid Arthritis (RA) is a chronic systemic inflammatory disease of unknown etiology, with chronic, symmetrical, polysynovial arthritis and extra-articular lesions as the main clinical manifestations, and belongs to autoimmune inflammatory disease disease. The disease is prone to occur in small joints such as hands, wrists, and feet, and is easy to recur, and is symmetrically distributed. In the early stage of the disease, joints are red, swollen, hot, painful, and dysfunctional. In the late stage, the joints may appear stiff and deformed in varying degrees, accompanied by atrophy of bones and skeletal muscles, and are easily disabled. From the perspective of pathological ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/28A61K31/365A61P29/00A61P19/02
Inventor 张卫东苏娟单磊李慧梁沈云享叶霁林珊曹邦静张淑琴
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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