Method for preparing corn protein active peptide by using composite carrier immobilized double enzymes

Abstract

The invention discloses a method for preparing a corn protein active peptide by using composite carrier immobilized double enzymes. The method comprises the following steps of: adding an alkaline protease solution into a sodium alginate solution, stirring, adding a trypsin solution, stirring, and standing to obtain the sodium alginate solution coated with the double protease solutions; dropping the sodium alginate solution coated with the double protease solutions into a CaCl2-chitosan mixed solution while continuously stirring drop by drop, hardening into spheres, filtering, and drying to obtain composite carrier immobilized double-enzyme gel beads; and preparing a corn alcohol-soluble protein ethanol solution, regulating pH, adding the composite carrier immobilized double-enzyme gel beads, reacting, filtering, and regulating the pH of a filtrate to obtain a corn protein active peptide solution. The preparation process is simple, convenient, non-toxic and safe, the composite carrier immobilized double-enzyme gel beads are very easy to separate and high in stability, the reusability can be realized, the activity loss of the stored enzymes is low, the corn protein active peptide obtained by degradation is high in activity, and the degree of hydrolysis of the corn alcohol-soluble protein is better than that obtained by degradation of a free enzyme.

Description

technical field [0001] The invention relates to a method for preparing corn protein active peptide, which belongs to the application field of immobilized enzyme technology. Background technique [0002] Zein is the main by-product of corn processing—the main component of corn gluten meal, accounting for about 68%. It is a protein with a special structure, and the content of lysine and tryptophan is very small. Due to the high proportion of non-polar amino acid residues and the lack of basic and acidic amino acids, zein is insoluble in water and has a rough taste, which limits its application in the food industry. [0003] Corn peptide refers to the protein hydrolyzate obtained after corn protein is degraded by protease, and then separated and refined. It is composed of short peptides with small molecular weight and high activity. It is rich in glutamic acid, leucine and proline. And alanine, this special amino acid composition makes corn peptide have special physical and ch...

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Problems solved by technology

Free protease is easily inactivated in organic media, and the enzyme protein is difficult to recover, which not only increases the cost of the preparation process, but also causes difficulties in the separation and purification of subsequent products, which cannot be applied on a large scale in actual industrial production; single-enzyme hydrolysis (free single or single Carrier-immobilized single enzyme method) zein has problems such as insufficient hydrolysis and low degree of hydrolysis; zein is a highly hydrophobic protein with low solubility in water phase, large enzyme consumption, and low hydrolysis efficiency; in seaweed In the technique of immobilizing a single protease with sodium alginate as a carrier, the gel beads obtained by using a single carrier of sodium alginate are unstable in solutions containing polyvalent anions and high-concentration electrolyte solutions, calcium ions are easy to fall off, the gel becomes soft, and even dissolves. Gel beads obtained by immobilizing enzymes on a single carrier of sodium bicarbonate have poor stability

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