Composite microbial preparation for watershed bioremediation as well as preparation method and application thereof
A technology of compound microorganisms and microbial preparations, applied in the field of compound microorganism preparations, can solve the problems of reducing the concentration of inoculants, liquid inoculants and powdery inoculants are easily washed away by water flow, and water quality treatment cannot achieve expected results, etc. Environmentally friendly, non-toxic and environmentally friendly, and the effect of improving acid resistance and thermal stability
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Embodiment 1
[0033] (1) Select commercially available Bacillus natto, Candida utilis, and Rhodop seudanonas palustris, and mix them at a ratio of 1:1:1 by volume .
[0034] (2) adding the compound microbial preparations into fresh water culture solution and seawater culture solution respectively for cultivation, sedimentation, and removing the supernatant by siphon method to obtain fresh water mixed bacterial solution D and seawater mixed bacterial solution H; 1000ml contains 1.5g of ammonium chloride, 0.5g of sodium chloride, 1.5g of potassium dihydrogen phosphate, 5.0g of peptone, 8.0g of glucose, 5.0g of yeast extract, and the pH is 7.5. The seawater culture solution contains 24g of sodium chloride per 1000ml, 7.0g of magnesium sulfate, 1.0g of ammonium nitrate, 0.7g of potassium chloride, 2.0g of potassium dihydrogen phosphate, 3.0g of disodium hydrogen phosphate, and the pH is 7.4.
[0035] (3) Mix the fresh water mixed bacterial solution D obtained in step 2), the seawater mixed bac...
Embodiment 2
[0040] (1) Select commercially available Bacillus natto, Candida utilis, and Rhodop seudanonas palustris, and mix them at a ratio of 1:1:1 by volume .
[0041](2) The compound microbial preparation is added to fresh water culture solution and seawater culture solution respectively for cultivation, sedimentation, and siphon method to remove the supernatant to obtain fresh water mixed bacterial solution D and seawater mixed bacterial solution H; 1000ml contains 1.5g of ammonium chloride, 0.5g of sodium chloride, 1.5g of potassium dihydrogen phosphate, 5.0g of peptone, 8.0g of glucose, 5.0g of yeast extract, and the pH is 7.5. The seawater culture solution contains 24g of sodium chloride per 1000ml, 7.0g of magnesium sulfate, 1.0g of ammonium nitrate, 0.7g of potassium chloride, 2.0g of potassium dihydrogen phosphate, 3.0g of disodium hydrogen phosphate, and the pH is 7.4.
[0042] (3) Mix the fresh water mixed bacterial solution D obtained in step 2), the seawater mixed bacteri...
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