Composite microbial preparation for watershed bioremediation as well as preparation method and application thereof

A technology of compound microorganisms and microbial preparations, applied in the field of compound microorganism preparations, can solve the problems of reducing the concentration of inoculants, liquid inoculants and powdery inoculants are easily washed away by water flow, and water quality treatment cannot achieve expected results, etc. Environmentally friendly, non-toxic and environmentally friendly, and the effect of improving acid resistance and thermal stability

Active Publication Date: 2013-12-11
青岛水清木华环境工程有限公司
View PDF3 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] With the continuous improvement of people's research and understanding of the use of microorganisms, the harmfulness of pathogenic bacteria has been paid more and more attention while utilizing their beneficial effects; in terms of watershed bioremediation, the existing microbial agents are generally liquid agents and powders However, both liquid and powder bacterial agents are easily washed away by the water flow, which reduces the concentration of the bacte

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composite microbial preparation for watershed bioremediation as well as preparation method and application thereof
  • Composite microbial preparation for watershed bioremediation as well as preparation method and application thereof
  • Composite microbial preparation for watershed bioremediation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Select commercially available Bacillus natto, Candida utilis, and Rhodop seudanonas palustris, and mix them at a ratio of 1:1:1 by volume .

[0034] (2) adding the compound microbial preparations into fresh water culture solution and seawater culture solution respectively for cultivation, sedimentation, and removing the supernatant by siphon method to obtain fresh water mixed bacterial solution D and seawater mixed bacterial solution H; 1000ml contains 1.5g of ammonium chloride, 0.5g of sodium chloride, 1.5g of potassium dihydrogen phosphate, 5.0g of peptone, 8.0g of glucose, 5.0g of yeast extract, and the pH is 7.5. The seawater culture solution contains 24g of sodium chloride per 1000ml, 7.0g of magnesium sulfate, 1.0g of ammonium nitrate, 0.7g of potassium chloride, 2.0g of potassium dihydrogen phosphate, 3.0g of disodium hydrogen phosphate, and the pH is 7.4.

[0035] (3) Mix the fresh water mixed bacterial solution D obtained in step 2), the seawater mixed bac...

Embodiment 2

[0040] (1) Select commercially available Bacillus natto, Candida utilis, and Rhodop seudanonas palustris, and mix them at a ratio of 1:1:1 by volume .

[0041](2) The compound microbial preparation is added to fresh water culture solution and seawater culture solution respectively for cultivation, sedimentation, and siphon method to remove the supernatant to obtain fresh water mixed bacterial solution D and seawater mixed bacterial solution H; 1000ml contains 1.5g of ammonium chloride, 0.5g of sodium chloride, 1.5g of potassium dihydrogen phosphate, 5.0g of peptone, 8.0g of glucose, 5.0g of yeast extract, and the pH is 7.5. The seawater culture solution contains 24g of sodium chloride per 1000ml, 7.0g of magnesium sulfate, 1.0g of ammonium nitrate, 0.7g of potassium chloride, 2.0g of potassium dihydrogen phosphate, 3.0g of disodium hydrogen phosphate, and the pH is 7.4.

[0042] (3) Mix the fresh water mixed bacterial solution D obtained in step 2), the seawater mixed bacteri...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of bioremediation of rivers, and in particular relates to a composite microbial preparation for watershed bioremediation as well as a preparation method and application thereof. The microbial preparation comprises active ingredients including bacillus natto, candida utilis and rhodopseudomonas palustris which are mixed together in a volume fraction ratio of 1:1:1; the microbes for water treatment are combined with zeolite to prepare a comprehensive preparation for water treatment; the specific gravity of the obtained comprehensive preparation for water treatment is greater than that of water, so that the obtained comprehensive preparation for water treatment quickly sinks to the bottom of water and can not be washed away by water flow easily; the zeolite is used as a carrier for the microbes to grow, and can provide certain nutrition substances required by growth for the microbes while adsorbing pollutants, and the microbes attached to the zeolite perform decomposition and anabolism on the pollutants to ensure that the zeolite restores the adsorptive property, so that a virtuous cyclic process is formed. The microbial agent provided by the invention can adapt to fresh water and seawater environments at the same time, and has an effective bioremediation effect on watercourses of certain coastal cities, wherein the water quality of the watercourses has fresh water and seawater alternating conditions along with seasonal changes.

Description

technical field [0001] The invention relates to the field of river bioremediation, in particular to a composite microbial preparation for river basin bioremediation, as well as its preparation and application. Background technique [0002] The water in the river is flowing, and the ecological environment of the water body changes frequently, which has a great impact on the biological population in the water body, and its catchment area is large, so the non-point source pollution is very large, and it is more susceptible to the impact of point source pollution. Compared with rivers, lakes have large water surface, large storage capacity, stable water environment, small flow, and small catchment area. Therefore, the non-point source pollution and point source pollution of lakes are relatively small, and the water environment is relatively stable. easier to manage. Therefore, the management of rivers and lakes is different. At present, the restoration of ecosystems is mainly ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N11/14C02F3/34C12R1/07C12R1/72C12R1/01
Inventor 邵立强李啸林祝国成汪永财李高王永军王雷雷
Owner 青岛水清木华环境工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products