Enzyme and bacterium complexing agent for degrading crop straws
A technology of crop straw and compounding agent, which is applied in application, animal feed, animal feed, etc., can solve the problems of insufficient carbon and nitrogen, high fiber degradation effect, and degradation effect not as good as fungus, etc., to ensure effective degradation and improve availability , the effect of shortening the fermentation time
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[0063] The composition of the PDA medium is: potato extract 1L, glucose 20g, KH 2 PO 4 3g,MgSO 4 1.5g, vitamin B 1 10mg, 15g agar, pH 6.0. The method for preparing the potato extract includes the following steps: weighing 200 g of potatoes, washing, peeling, cutting into small pieces, adding 1000 mL of water, boiling for 30 minutes, filtering into a clear liquid with gauze, and adding water to 1000 mL.
[0064] Other carriers, feed additives and raw materials can be purchased from the feed market.
Embodiment 1
[0065] Example 1: An enzyme-bacteria composite agent for degrading crop straw
[0066] 1. Composition: 45 parts of microbial compound agent, 10 parts of compound enzyme, 45 parts of carrier. among them:
[0067] Microbial compound: 25 parts of Phanerochaete chrysosporium, 25 parts of Aspergillus niger, 25 parts of Penicillium, 25 parts of Trichoderma viride; each bacteria contains 25ml culture medium, the number of viable bacteria in the culture medium is 10 8 Pieces / mL.
[0068] Compound enzyme: 35 parts of xylanase, 20 parts of cellulase, 10 parts of pectinase;
[0069] Carrier: 75 parts of glucose and 25 parts of calcium chloride.
[0070] 2. Preparation method:
[0071] 1) Expand each single strain with PDA medium to the number of viable bacteria in the culture solution to 10 8 Pcs / mL, spare;
[0072] 2) Weigh Phanerochaete chrysosporium, Aspergillus niger, Penicillium, and Trichoderma viride culture medium according to the ratio. After mixing evenly, centrifuge the fermented strain ...
Embodiment 2
[0076] Example 2: An enzyme-bacteria composite agent for degrading crop straw
[0077] 1. Composition: 30 parts of microbial compound agent, 15 parts of compound enzyme, 55 parts of carrier.
[0078] Microbial compound agent: 33 parts of Phanerochaete chrysosporium, 33 parts of Aspergillus niger, 33 parts of Penicillium; each bacteria contains 25ml culture solution, the number of viable bacteria in the culture solution is 10 8 Pieces / mL.
[0079] Compound enzyme: 40 parts xylanase, 10 parts cellulase, 20 parts pectinase;
[0080] Carrier: 50 parts glucose, 50 parts calcium chloride.
[0081] 2. Preparation method: same as in Example 1.
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