Enzyme and bacterium complexing agent for degrading crop straws

A technology of crop straw and compounding agent, which is applied in application, animal feed, animal feed, etc., can solve the problems of insufficient carbon and nitrogen, high fiber degradation effect, and degradation effect not as good as fungus, etc., to ensure effective degradation and improve availability , the effect of shortening the fermentation time

Active Publication Date: 2014-02-05
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fungi have a good decomposing effect on cellulose and lignin, and although bacteria also have the corresponding ability to produce cellulase, the degradation effect is not as good as that of fungi, especially in straw fermentation, when straw lignin has not been degraded, bacteria The amount of carbon and nitrogen required for growth and expansion is insufficient to maintain the activity of bacteria
At the same time, too many strains are used, which not only increases the cost, but also does not guarantee a high fiber degradation effect
[0017] Existing s...

Method used

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  • Enzyme and bacterium complexing agent for degrading crop straws
  • Enzyme and bacterium complexing agent for degrading crop straws
  • Enzyme and bacterium complexing agent for degrading crop straws

Examples

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preparation example Construction

[0063] The composition of the PDA medium is: potato extract 1L, glucose 20g, KH 2 PO 4 3g,MgSO 4 1.5g, vitamin B 1 10mg, 15g agar, pH 6.0. The method for preparing the potato extract includes the following steps: weighing 200 g of potatoes, washing, peeling, cutting into small pieces, adding 1000 mL of water, boiling for 30 minutes, filtering into a clear liquid with gauze, and adding water to 1000 mL.

[0064] Other carriers, feed additives and raw materials can be purchased from the feed market.

Embodiment 1

[0065] Example 1: An enzyme-bacteria composite agent for degrading crop straw

[0066] 1. Composition: 45 parts of microbial compound agent, 10 parts of compound enzyme, 45 parts of carrier. among them:

[0067] Microbial compound: 25 parts of Phanerochaete chrysosporium, 25 parts of Aspergillus niger, 25 parts of Penicillium, 25 parts of Trichoderma viride; each bacteria contains 25ml culture medium, the number of viable bacteria in the culture medium is 10 8 Pieces / mL.

[0068] Compound enzyme: 35 parts of xylanase, 20 parts of cellulase, 10 parts of pectinase;

[0069] Carrier: 75 parts of glucose and 25 parts of calcium chloride.

[0070] 2. Preparation method:

[0071] 1) Expand each single strain with PDA medium to the number of viable bacteria in the culture solution to 10 8 Pcs / mL, spare;

[0072] 2) Weigh Phanerochaete chrysosporium, Aspergillus niger, Penicillium, and Trichoderma viride culture medium according to the ratio. After mixing evenly, centrifuge the fermented strain ...

Embodiment 2

[0076] Example 2: An enzyme-bacteria composite agent for degrading crop straw

[0077] 1. Composition: 30 parts of microbial compound agent, 15 parts of compound enzyme, 55 parts of carrier.

[0078] Microbial compound agent: 33 parts of Phanerochaete chrysosporium, 33 parts of Aspergillus niger, 33 parts of Penicillium; each bacteria contains 25ml culture solution, the number of viable bacteria in the culture solution is 10 8 Pieces / mL.

[0079] Compound enzyme: 40 parts xylanase, 10 parts cellulase, 20 parts pectinase;

[0080] Carrier: 50 parts glucose, 50 parts calcium chloride.

[0081] 2. Preparation method: same as in Example 1.

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PUM

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Abstract

The invention relates to an enzyme and bacterium complexing agent for degrading crop straws. The enzyme and bacterium complexing agent comprises the active components including a microorganism complexing agent and compound enzyme. An experiment result shows that xylogen, neutral detergent fibers, acid detergent fibers, acid detergent xylogen, cellulose, crude protein and the like of the straws treated by the enzyme and bacterium complexing agent are well degraded; the degrading rate of rumens of ruminants to the xylogen of the straws is increased, and the surrounding of the xylogen around other nutritional components is broken through, so that the availability of the straws is improved. The enzyme and bacterium complexing agent disclosed by the invention is convenient to use; the straws are not required to be subjected to physical pretreatment; the fermentation time of the straws is short; an effect of the complexing agent is better than that in the prior art.

Description

Technical field [0001] The invention relates to the field of processing and utilization of ruminant coarse feed in the feed industry, in particular to an enzyme-bacteria composite agent for degrading crop straw. Background technique [0002] my country is a large agricultural country with extremely rich crop straw resources. The annual output of straw in the world is as much as 3 billion tons. In 2009, the remaining straw output after the crops were harvested in my country was as high as 740 million tons, ranking among the top in the world. However, due to the special physical and chemical properties of crop straw, its use as feed has many limitations. The straw has a rough texture, poor palatability, poor nutritional value, high lignin and cellulose content, and low digestibility and utilization rate. Only a small part of it can be used to feed ruminants. Monogastric animals generally cannot use straw, and the digestibility of straw by ruminants is only 20%-30%. How to improv...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/00A23K1/165
Inventor 刁其玉屠焰张立霞
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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