Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Non-labeled ionic conjugated polyelectrolyte, synthetic method thereof and application to biological detection

A conjugated polyelectrolyte and polyelectrolyte technology, applied in chemical instruments and methods, color/spectral characteristic measurement, fluorescence/phosphorescence, etc., to achieve low cost, excellent water solubility, and good light/thermal stability

Inactive Publication Date: 2014-02-19
NANJING UNIV OF POSTS & TELECOMM
View PDF1 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Later, people began to use intercalation dyes that do not need to be labeled in advance. This intercalation dye can be well intercalated into dsDNA, but this feature also limits this detection strategy to DNA and not to other biomolecules.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Non-labeled ionic conjugated polyelectrolyte, synthetic method thereof and application to biological detection
  • Non-labeled ionic conjugated polyelectrolyte, synthetic method thereof and application to biological detection
  • Non-labeled ionic conjugated polyelectrolyte, synthetic method thereof and application to biological detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0039] In a 25 mL Schlenk tube, 9,9-bis(3,3'-dimethyl-1-propylamino)-fluorene-2,7-bisboronic acid pinacol ester (588.4 mg, 1.0 mmol), 9 ,9-bis(3,3'-dimethyl-1-propylamino)-2,7-dibromofluorene (345.8mg,0.7mmol), 4,7-dibromo-benzothiadiazole (88mg, 0.3mmol), 3.5mg tetrakis (triphenylphosphine) palladium, dissolved in 12 ml freshly distilled tetrahydrofuran and 6 ml saturated potassium carbonate mixed solution. Protected from light, heated and stirred at 85°C for 36 hours under the protection of argon. The obtained yellow polymer solution was settled in ether, the solid was collected, dissolved in ethanol, and settled again with 350 ml of ether to obtain a yellow filamentary solid, which was a neutral polymer.

[0040] Take 30 mg of neutral polymer, dissolve it in 5 ml of dimethyl sulfoxide, add 3 ml of ethyl bromide under heating and stirring at 45°C, wait for precipitation to form, add 20 ml of acetone, and add 3 ml of ethyl bromide while stirring continuously. After 40 hours...

Embodiment 1

[0055] Add 4.5 μM P1 (concentration is calculated by the average number of repeating units) to the pure water system, and measure its ultraviolet absorption spectrum. which is figure 1 .

Embodiment 2

[0057] Add 4.5 μM P1 to pure water, Tris buffer solution (pH=7.4) and PBS buffer solution (pH=7.4) respectively, and measure the fluorescence emission spectrum. which is figure 2 . The excitation wavelength is 381nm, and the slit is (3;5).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses design, detection application of non-labeled ionic conjugated polyelectrolyte. The non-labeled ionic conjugated polyelectrolyte has a structure shown as the general formula (I) shown in the description, and comprises a 9-substituted fluorenes unit and an acceptor unit Ar with electron-withdrawing characteristic, Ar is one substituent groups of benzo[d]thiadiazol, benzo[d]thiazol, thiazole, benzo[d]diazol, boron-dipyrromethene unit, iridium complex containing unit, metalloporphyrin containing unit, perylene bisimide, naphthalimide and the like, the ratio of fluorenes group to Ar (x:y) is 17:3-18:2, the side group comprises m+1 CH2 units, wherein Ion is one water-soluble groups such as quaternary ammonium group, carboxyl or sulfo group. The non-labeled ionic conjugated polyelectrolyte has extremely high solubility and stability, is applicable to multiple non-labeled biological detection, and is a brand-new sensing detection platform.

Description

technical field [0001] The present invention relates to the design of a class of conjugated polyelectrolytes, specifically the design of a class of non-marked conjugated polyelectrolytes composed of fluorenyl and another luminescent material and its use in surfactants, DNA, protein active substances biological detection applications. Background technique [0002] The structure of the novel material of the conjugated polymer makes it have a large number of absorption units. Excitation energy can migrate along the polymer backbone and then to the chromophore, which will result in spectral signal amplification. Therefore, conjugated polymers can act as light-harvesting materials and light amplification tools based on fluorescence resonance energy transfer (FRET). Owing to these remarkable properties, conjugated polymers have been used as optical platforms for highly sensitive chemical and biological sensors. [0003] For biological detection, water-soluble, charged (anion, c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08G61/12C09K11/06G01N21/33G01N21/64
Inventor 傅妮娜郝疏影汪联辉翁丽星
Owner NANJING UNIV OF POSTS & TELECOMM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com