Non-labeled ionic conjugated polyelectrolyte, synthetic method thereof and application to biological detection

A conjugated polyelectrolyte and polyelectrolyte technology, applied in chemical instruments and methods, color/spectral characteristic measurement, fluorescence/phosphorescence, etc., to achieve low cost, excellent water solubility, and good light/thermal stability

Inactive Publication Date: 2014-02-19
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Later, people began to use intercalation dyes that do not need to be labeled in advance. This intercalation dye can be

Method used

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  • Non-labeled ionic conjugated polyelectrolyte, synthetic method thereof and application to biological detection
  • Non-labeled ionic conjugated polyelectrolyte, synthetic method thereof and application to biological detection
  • Non-labeled ionic conjugated polyelectrolyte, synthetic method thereof and application to biological detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0039] In a 25 mL Schlenk tube, 9,9-bis(3,3'-dimethyl-1-propylamino)-fluorene-2,7-bisboronic acid pinacol ester (588.4 mg, 1.0 mmol), 9 ,9-bis(3,3'-dimethyl-1-propylamino)-2,7-dibromofluorene (345.8mg,0.7mmol), 4,7-dibromo-benzothiadiazole (88mg, 0.3mmol), 3.5mg tetrakis (triphenylphosphine) palladium, dissolved in 12 ml freshly distilled tetrahydrofuran and 6 ml saturated potassium carbonate mixed solution. Protected from light, heated and stirred at 85°C for 36 hours under the protection of argon. The obtained yellow polymer solution was settled in ether, the solid was collected, dissolved in ethanol, and settled again with 350 ml of ether to obtain a yellow filamentary solid, which was a neutral polymer.

[0040] Take 30 mg of neutral polymer, dissolve it in 5 ml of dimethyl sulfoxide, add 3 ml of ethyl bromide under heating and stirring at 45°C, wait for precipitation to form, add 20 ml of acetone, and add 3 ml of ethyl bromide while stirring continuously. After 40 hours...

Embodiment 1

[0055] Add 4.5 μM P1 (concentration is calculated by the average number of repeating units) to the pure water system, and measure its ultraviolet absorption spectrum. which is figure 1 .

Embodiment 2

[0057] Add 4.5 μM P1 to pure water, Tris buffer solution (pH=7.4) and PBS buffer solution (pH=7.4) respectively, and measure the fluorescence emission spectrum. which is figure 2 . The excitation wavelength is 381nm, and the slit is (3;5).

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Abstract

The invention discloses design, detection application of non-labeled ionic conjugated polyelectrolyte. The non-labeled ionic conjugated polyelectrolyte has a structure shown as the general formula (I) shown in the description, and comprises a 9-substituted fluorenes unit and an acceptor unit Ar with electron-withdrawing characteristic, Ar is one substituent groups of benzo[d]thiadiazol, benzo[d]thiazol, thiazole, benzo[d]diazol, boron-dipyrromethene unit, iridium complex containing unit, metalloporphyrin containing unit, perylene bisimide, naphthalimide and the like, the ratio of fluorenes group to Ar (x:y) is 17:3-18:2, the side group comprises m+1 CH2 units, wherein Ion is one water-soluble groups such as quaternary ammonium group, carboxyl or sulfo group. The non-labeled ionic conjugated polyelectrolyte has extremely high solubility and stability, is applicable to multiple non-labeled biological detection, and is a brand-new sensing detection platform.

Description

technical field [0001] The present invention relates to the design of a class of conjugated polyelectrolytes, specifically the design of a class of non-marked conjugated polyelectrolytes composed of fluorenyl and another luminescent material and its use in surfactants, DNA, protein active substances biological detection applications. Background technique [0002] The structure of the novel material of the conjugated polymer makes it have a large number of absorption units. Excitation energy can migrate along the polymer backbone and then to the chromophore, which will result in spectral signal amplification. Therefore, conjugated polymers can act as light-harvesting materials and light amplification tools based on fluorescence resonance energy transfer (FRET). Owing to these remarkable properties, conjugated polymers have been used as optical platforms for highly sensitive chemical and biological sensors. [0003] For biological detection, water-soluble, charged (anion, c...

Claims

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Application Information

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IPC IPC(8): C08G61/12C09K11/06G01N21/33G01N21/64
Inventor 傅妮娜郝疏影汪联辉翁丽星
Owner NANJING UNIV OF POSTS & TELECOMM
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