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Tissue engineering bracket imitating intima-media structure and function of natural blood vessels and preparation method thereof

A technology of tissue engineering scaffold and membrane structure, applied in medical science, prosthesis, etc., can solve the problems of vascular smooth muscle cell adhesion, growth, spatial organization and function, etc., achieve good biocompatibility, realize Mechanical properties, the effect of good mechanical properties

Active Publication Date: 2014-03-26
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This single surface topography scaffold is not ideal for controlling the adhesion, growth, spatial organization and function of VSMCs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] The preparation method of the tissue engineering scaffold imitating the structure and function of the natural vascular media layer provided by the present invention, the steps are as follows: 1) Preparation of growth factor-loaded nanoparticles: soak the nanoparticles in growth factor A and / or B solution for a period of time After centrifugation, washing and freeze-drying, growth factor-loaded nanoparticles were obtained.

[0037] The materials for preparing nanoparticles are gelatin, collagen or silicon dioxide.

[0038] The gelatin nanoparticle and the collagen nanoparticle are prepared by the emulsion-condensation method, and the particle diameter is 400-1000 nanometers. The emulsification temperature adopted is 40° C., the stirring speed is 4-6 thousand revolutions per minute, and the emulsification time is 2-10 minutes.

[0039] The silica nanoparticles are prepared by a sol-gel method, and the particle diameter is 30-300 nanometers.

[0040] The growth factor A,...

Embodiment 1

[0049] Example 1. Preparation of a tissue engineering scaffold imitating the structure and function of the natural vascular media layer

[0050] The first step: preparation of growth factor-loaded bFGF gelatin particles: 5ml of mass concentration of 10% gelatin aqueous solution was added dropwise in 175ml of olive oil, stirred at 40°C and 5,000 rpm for 10 minutes, then the emulsion was cooled to room temperature, Stirring was continued for 30 minutes, 100 ml of acetone was added, and stirring was continued for 1 h at 5,000 rpm. The resulting particles were washed with acetone and isopropanol at 4°C, respectively, and collected by centrifugation. The obtained particles were put into 2% glutaraldehyde solution containing 0.1% Tween 60, and cross-linked at 4°C for 12 hours. The particles were then put into 100 ml of 10 mM amino acid solution containing 0.1% Tween 60 and stirred at 37° C. for 30 minutes. After washing with Tween 60 solution with a mass concentration of 0.1%, it i...

Embodiment 2

[0059] Example 2. Preparation of a tissue engineering scaffold imitating the structure and function of the natural vascular media layer

[0060] Step 1: According to the method of Example 1, gelatin particles loaded with growth factor bFGF were prepared.

[0061] The second step: according to the method of Example 1, silica nanoparticles loaded with growth factor TGF-β1 were prepared.

[0062] The third step: PGLC (GA / L-LA / CL=63 / 27 / 10, mol / mol / mol) (the number average molecular weight is 80,000) was prepared into 630 μl of 6% tetrahydrofuran solution, and 40 μl of trihydrofuran was added. Methyl chloride was mixed evenly, and 8 mg of nano-gelatin particles loaded with bFGF and 8 mg of silica nanoparticles of TGF-β1 were added, stirred evenly, and cast onto PDMS with parallel grooves on the surface of 3×3 cm2 in size, and the groove width was 80 microns, the groove depth is 5 microns, and the groove pitch is 10 microns. The solvent is evaporated by freeze-drying to obtain a p...

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Abstract

The invention discloses a tissue engineering bracket imitating intima-media structures and functions of natural blood vessels, and a preparation method of the bracket. The bracket is a porous membranoid substance which is prepared from degradable macromolecules, loaded with growth factors, provided with micron-order grooves in parallel in the surface and is of a nano topological structure on the outer surface. The preparation method comprises the following steps: uniformly stirring nano grains with growth factors and a biologic degradable polymer solution, subsequently casting on a mold surface with the grooves in parallel, freezing and drying to eliminate the solvent, further performing surface treatment and loading the growth factors so as to obtain the tissue engineering bracket. Due to the parallel micron-order groove structure, the surface nano topological structure and the loaded growth factors of the tissue engineering bracket disclosed by the invention, induction and prompting functions on growth and breading of vascular smooth muscle cells, which are similar to those of extracellular matrix of intima-media cells of the natural blood vessels, can be achieved, and the vascular smooth muscle cells are enabled to grow and breed in ordered orientation on the artificial bracket like the intima-media of the natural blood vessels.

Description

technical field [0001] The invention relates to a tissue engineering support imitating the structure and function of the media layer of natural blood vessels and a preparation method thereof. Background technique [0002] For vascular tissue engineering, the biodegradable scaffold supports and induces cell growth and reproduction, regulates the shape and size of the regenerated tissue, and finally combines with the body tissue. Three elements. At present, although there are many research reports on vascular tissue engineering using scaffolds of different materials, the structure and function of these scaffolds are far from the extracellular matrix of natural blood vessels, and so far there is no similar structure and function to natural blood vessels. A report on tissue engineered vascular stents. [0003] The vascular wall is composed of adventitia, media and intima, in which the media is composed of highly oriented layers of vascular smooth muscle cells, and in these cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/56A61L27/18
Inventor 沈红吴德成杨飞王身国
Owner INST OF CHEM CHINESE ACAD OF SCI
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