Large-grain milk replacer and preparing method thereof
A technology of creep feed and large particles, applied in animal feed, animal feed, application, etc., to achieve the effects of rich amino acid content, reduced feed waste, and high enzyme activity
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[0039] (1) Preparation of bacterial suspension
[0040] Inoculate the Li-2013 single colony grown after streaking on the plate into the seed medium, culture at 40°C for 12 hours at 100 r / min, take 1 mL of the culture medium, centrifuge, wash twice with normal saline, and resuspend with 9 mL of physiological in salt water.
[0041] (2) Ultraviolet-Lithium Chloride-Diethyl Sulfate Compound Mutagenesis
[0042] The bacterial suspension was placed on a sterile plate, stirred and irradiated for 100s under a UV lamp with a distance of 30cm and a power of 15w. The irradiated bacteria solution was diluted and spread on the lithium chloride plate after gradient dilution, and the bacteria solution without ultraviolet irradiation was diluted and spread on the plate as a control. Wrap the uniformly coated plate above with black cloth or newspaper, incubate at 40°C for 48 hours, screen out the plate with the largest ratio of hydrolysis circle to colony diameter on the plate that grows co...
Embodiment 1
[0061] A kind of large-grained creep material, comprising the following raw materials in parts by weight:
[0062] Peeled corn 35, puffed soybean 9, flour 8, millet 2, super steam fish meal 4, peeled soybean meal 4, yeast extract 2, fermented soybean meal 5, egg powder 3, milk powder 5, lactose 6, glucose 2, sugar 1 , Acidifier 4, calcium dihydrogen phosphate 0.4, amino acid 0.3, high temperature protease 0.02, high temperature amylase and its culture 2, compound vitamin 0.05, compound trace element 0.4.
[0063] The amino acid mass ratio is: Dlys:Dmet:Dthr:Dtrp:Darg:Dval:Dtyr:Dile=100:38:65:21:70:55:30:45.
[0064] The composition of the multivitamins is: 1 part of vitamin A, 2 parts of vitamin D, 2 parts of vitamin E, and 2 parts of vitamin B 1 0.2 parts, vitamin B 2 0.1 part, vitamin B 6 0.3 parts.
[0065] The composite trace elements are composed of: 3 parts of ferrous sulfate, 1 part of manganese sulfate, 1 part of zinc oxide, 1 part of basic copper chloride, 0.15 pa...
Embodiment 2
[0083] A kind of large-grained creep material, comprising the following raw materials in parts by weight:
[0084] Peeled corn 31, puffed soybean 12, flour 6, millet 1, super steam fish meal 3, peeled soybean meal 5, yeast extract 3, fermented soybean meal 4, egg powder 2, milk powder 3, lactose 4, glucose 3, white sugar 3 , Acidifier 3, calcium dihydrogen phosphate 0.4, amino acid 0.2, high temperature protease 0.01, high temperature amylase and its culture 1, compound vitamin 0.06, compound trace element 0.6.
[0085] The amino acid mass ratio is: Dlys:Dmet:Dthr:Dtrp:Darg:Dval:Dtyr:Dile=100:38:65:21:70:55:30:45.
[0086] The composition of the multivitamin is: 2 parts of vitamin A, 2 parts of vitamin D, 3 parts of vitamin E, vitamin B 1 0.3 parts, vitamin B 2 0.2 parts, vitamin B 6 0.5 servings.
[0087] The composite trace elements are composed of: 2 parts of ferrous sulfate, 1 part of manganese sulfate, 2 parts of zinc oxide, 1 part of basic copper chloride, 0.2 part o...
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