Bacillus subtilis strain and applications thereof
A technology of Bacillus subtilis and strains, applied in the direction of bacteria, biochemical equipment and methods, microorganisms, etc., can solve the problem of not being able to use glycerin, and achieve the effect of cheap raw materials, high output, and short fermentation cycle
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0034] Example 1
[0035] This example illustrates the obtaining of Bacillus subtilis G-34 ( Bacillus subtilis G-34) method.
[0036] It includes the following steps:
[0037] 1) Obtain biological surface active strains in petroleum-contaminated soil by enriching the medium.
[0038] Take samples of petroleum-contaminated soil from Shengli Oilfield, and inoculate the processed soil samples into enrichment medium to obtain enrichment culture solution. Taking advantage of the hemolytic properties of the active agent of the biological surface, the enriched culture solution is inoculated on the blood plate by streaking and coating separation method, and cultured at 37°C for 24-48h to obtain a single colony with a large hemolytic circle and a transparent.
[0039] The enrichment medium formula is: glucose 20 g / L, ammonium sulfate 1 g / L, sodium nitrate 2 g / L, magnesium sulfate 0.3 g / L, potassium dihydrogen phosphate 1 g / L, and disodium hydrogen phosphate 4 g / L, yeast powder 2 g / L.
[0040...
Example Embodiment
[0050] Example 2
[0051] This example illustrates the treatment of Bacillus subtilis strains Bacillus subtilis The result of preliminary identification of the type of surfactant produced by G-34.
[0052] To Bacillus subtilis Strain G-34 is a strain, and glycerol is used as a carbon source for shake flask fermentation. Bacillus subtilis The biosurfactant produced by G-34 was purified and analyzed by FT-IR.
[0053] The analysis results are attached figure 1 . In the IR spectrum, 3303cm-1 is the NH stretching vibration caused by the hydrogen bonds between molecular chains, and 1656cm-1 and 1550cm-1 are the thalidomide bands I and II. The hydrophilic group of the above-mentioned characteristic absorption surface surfactant molecule is a peptide chain. The two absorptions of the spectrum 2872-2960cm-1 and 1243-1402cm-1 are the CH stretching vibration table of the fatty acid family. The hydrophobic part of the surfactant molecule is a fat half molecule, indicating that the surfac...
Example Embodiment
[0054] Example 3
[0055] This example illustrates Bacillus subtilis Bacillus subtilis G-34 uses pure glycerol to ferment to produce lipopeptide biosurfactants.
[0056] (1) Preparation of seed culture medium: yeast extract 5 g / L, peptone 10 g / L, and NaCl 10 g / L. Sterilize at 121 ℃ for 20 min, and cool for later use.
[0057] (2) Preparation of fermentation medium: glycerol 20 g / L, NH 4 NO 3 2 g / L, KH 2 PO 4 3 g / L, Na 2 HPO 4 10 g / L, MgSO 4 ·7H 2 O 0.2 g / L, FeSO 4 ·7H 2 O 0.02 g / L.
[0058] The medium was adjusted to pH 7.0 with phosphoric acid (1mol / L), sterilized at 121°C for 20 minutes, and cooled for later use.
[0059] (3) Pick a ring Bacillus subtilis The G-34 strain was placed in the medium obtained in step (1); placed at 37° C. and cultured at 200 rpm for 12 hours to obtain seed liquid.
[0060] (4) Take 1 ml of the Bacillus subtilis G-34 seed solution obtained in step (3) and inoculate 50 ml of the fermentation medium obtained in step (2). Incubate at 37°C and 200 rpm.
...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap