Composite microbial preparation, and preparation method and application thereof

A technology of compound microorganisms and preparations, which is applied in the field of microecological preparations and feed additives, to achieve the effects of not being easy to separate and separate out, improving the production performance of livestock and poultry, and stimulating the body's immune response

Active Publication Date: 2014-08-13
WUHAN KEQIAN BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many feed microecological preparations on the market, the effects of which are mainly focused on providing animal immunity and increasing animal

Method used

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  • Composite microbial preparation, and preparation method and application thereof
  • Composite microbial preparation, and preparation method and application thereof
  • Composite microbial preparation, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Isolation and identification of Enterococcus faecium SC13:

[0027] The applicant isolated, screened and identified the fresh feces of healthy pigs collected from a natural pig farm in Huangpi, Hubei, and preserved it in the China Center for Type Culture Collection on March 3, 2014, with the preservation number CCTCC NO: M2014060.

[0028] The strain is Gram-positive, with oval cells, mainly in pairs, and occasionally in short chains. It can grow at 10°C and 45°C. The final pH of fermenting glucose to produce acid is 4.0-4.4. It can grow in 6.5% NaCl and In the pH9.6 environment, peroxidase is negative, can decompose sucrose, mebiose, lactose, maltose, raffinose, arabinose and sorbitol, decompose arginine to produce ammonia, and the bile-escin hydrolysis test is not positive, It does not decompose rhamnose and melezitose, and needs folic acid to grow on the basic synthetic medium. According to the Bergey's Bacteria Handbook (Eighth Edition), this bacterium conforms to t...

Embodiment 2

[0050] A compound microbial preparation for livestock and poultry, the preparation steps of which are as follows:

[0051] Preparation of Bacillus amyloliquefaciens bacteria powder: Bacillus amyloliquefaciens (CCTCC NO: M2013249) was inoculated in self-prepared fermentation medium, and fermented with aeration and stirring at 30°C for 44 hours, real-time defoaming and controlled dissolved oxygen above 30%, until spores formed rate reached 75%. After putting into the tank, it is micro-filtered by ceramic membrane, concentrated at a pressure of 0.2 MPa to 1 / 5 of the original volume, and then put into a drying tower, and spray-dried in a spray drying tower at 165°C-175°C and 60°C-70°C. Obtain Bacillus amyloliquefaciens bacteria powder, the number of live bacteria is 2×10 10 CFU / g, for the following examples;

[0052] Fermentation medium components / L: corn flour 20g / L, soybean meal 10-50g / L, yeast extract 15g / L, peptone 10g / L, CaCO 3 2.5g / L, NaCl5g / L, MgSO 4 3g / L, KH 2 PO 4 5...

Embodiment 3

[0060] A kind of compound microbial preparation for livestock and poultry is used in the application of preparation treatment diarrhea drug, and its application process is as follows:

[0061] From a diarrhea-affected pig farm in Huangpi, 240 large white × landrace binary hybrid 26-day-old weaned piglets with an average body weight of 7.44±0.25kg were selected and randomly divided into 3 groups, with 80 pigs in each group. , respectively feeding the basal diet (blank control group), the feed additive (experimental group 1) prepared by the embodiment 2 of the basal diet+0.05% (w / w), the basal diet+0.2% (w / w) The feed additive prepared in Example 2, the test period was 28 days. At the beginning and end of the test, the individual fasting weight of the piglets was weighed, and the average daily gain (ADG), the feed amount, the feed mass ratio (F / G), diarrhea rate and mortality were calculated.

[0062] As can be seen from Table 6, during the test, the average weight gain of test...

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Abstract

The invention discloses a composite microbial preparation and a preparation method and application thereof. The microbial preparation contains Enterococcus faecium SC13 with an accession number of CCTCC NO: M2014060 and Kluyveromyces marxianus C2 with an accession number of CCTCC NO: M2014059 which are separated by the inventor. Enterococcus faecium SC13 and Kluyveromyces marxianus C2 are combined with Bacillus amyloliquefaciens, jerusalem artichoke powder and food-grade diatomite; through cooperation among bacteria and between the bacteria and a filler, the immune system of an animal is strengthened, proliferation of beneficial bacteria in the intestines of the animal is selectively promoted, colonization and propagation of harmful bacteria are inhibited, the microbial environment in the body of the animal is improved, immune response of organisms is stimulated, immunity and production performance of livestock and poultry are enhanced, usage of antibiotics is reduced, good recognition adhesion and elimination capability on mycotoxins in a feed is obtained, and the conversion rate of the feed is increased.

Description

technical field [0001] The invention relates to a microbial compound preparation, in particular to a microbial compound preparation for preventing diarrhea and detoxification of livestock and poultry and a preparation method thereof, belonging to the technical field of microecological preparations and feed additives. Background technique [0002] The accumulation of toxins in the body and diarrhea are two important reasons for the high mortality of livestock and poultry and the low return rate of breeding. About 25% of the grains in the world are polluted by various molds every year. Due to the different levels of pollution, there are great differences between different regions. China is the hardest hit area of ​​mycotoxins. According to the investigation by China Animal Protection Association, during the breeding process, a series of problems such as slow growth of fattening pigs, false estrus of sows, diarrhea of ​​suckling piglets, decreased egg production rate of laying ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A23K1/16C12R1/07C12R1/46C12R1/645A23K10/18
Inventor 徐高原王杨波周明光金建云陈章表李芳韩进蔡皓金梅林陈焕春
Owner WUHAN KEQIAN BIOLOGY CO LTD
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