Feeding high temperature-resistant microcapsule yeast probiotics preparation as well as preparation method and application thereof

A probiotic preparation, high temperature resistant technology, applied in the field of feed additives, can solve the problems of inactivation, enterococcus inactivation, loss of live yeast cells, etc., and achieve high survival rate, good heat resistance, and good storage stability

Active Publication Date: 2014-08-27
翁源县新南都饲料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the domestic microecological preparations are freeze-dried powders. After adding microecological powders during the processing of pellet feed and extruded feed, 10% to 30% of spores can be inactivate

Method used

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  • Feeding high temperature-resistant microcapsule yeast probiotics preparation as well as preparation method and application thereof
  • Feeding high temperature-resistant microcapsule yeast probiotics preparation as well as preparation method and application thereof
  • Feeding high temperature-resistant microcapsule yeast probiotics preparation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0050] Example 1

[0051] Using modern microbial separation technology, a probiotic strain of yeast is isolated from the intestinal contents of healthy pigs. The separation steps are as follows: collect chyme samples from the intestines of healthy piglets just slaughtered 14 days old under aseptic conditions, place them in sterile glass bottles and transfer them to the laboratory under ice bath conditions for later use, and then take a little food under aseptic conditions The minced sample was inoculated into YPD medium and cultured with shaking at 30°C and 200rpm for 36 hours. After dilution, it was spread on YPD culture plate, colony morphology was observed and combined with ordinary microscope inspection, single colony was picked and streaked, and a batch of yeast cells was selected. Strains, the growth performance of each strain was compared by shaking flask culture, and a yeast strain with excellent traits was screened and identified by 18S rDNA molecular identification. The...

Example Embodiment

[0055] Example 2 Preparation of a high-temperature resistant microcapsule yeast probiotic preparation for feed

[0056] (1) Preparation of Saccharomyces cerevisiae paste

[0057] The slant Saccharomyces cerevisiae strain was inoculated into the wort liquid medium (130g / L malt extract powder, autoclaved at 121°C for 20 minutes and stored for later use), pH 5.0, culture temperature at 30°C, shaking culture for 24 hours to obtain liquid seeds Culture solution, inoculate the liquid seed culture solution into 20L fermentation medium at a ratio of 5% (v / v), pH 5.5, temperature 30°C, and supplement appropriate carbon source, nitrogen source and phosphorus source by feeding, and cultivate for 36 hours , To obtain Saccharomyces cerevisiae milk, add 2.5g / L NaCl, 4g / L MgCl to the yeast milk 2 ·6H 2 After O and 1.5g / L Tween-80, vacuum filtration is used to obtain Saccharomyces cerevisiae mud;

[0058] The steps of feeding appropriate carbon source, nitrogen source and phosphorus source in the f...

Example Embodiment

[0067] Example 3 Preparation of a high-temperature-resistant granular formulation for feed of Saccharomyces cerevisiae

[0068] (1) Preparation of Saccharomyces cerevisiae paste

[0069] The slant Saccharomyces cerevisiae strain was inoculated into the wort liquid medium (130g / L malt extract powder, autoclaved at 121°C for 20 minutes and stored for later use), pH 5.0, culture temperature at 30°C, shaking culture for 36 hours to obtain liquid seeds Culture solution, inoculate the liquid seed culture solution into 20L fermentation medium at a ratio of 10% (v / v), pH 5.0, temperature 30°C, and supplement appropriate carbon source, nitrogen source and phosphorus source by feeding method. 24h, get Saccharomyces cerevisiae milk, add 2.5g / L NaCl, 4g / L MgCl to the yeast milk 2 ·6H 2 After O and 1.5g / L Tween-80, vacuum filtration is used to obtain Saccharomyces cerevisiae mud;

[0070] The steps of feeding appropriate carbon source, nitrogen source and phosphorus source in the feeding method ...

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Abstract

The invention discloses a feeding high temperature-resistant microcapsule yeast probiotics preparation as well as a preparation method and application thereof, and belongs to the technical field of feed additives. The feeding high temperature-resistant microcapsule yeast probiotics preparation is prepared by the steps such as fermenting, centrifuging, cleaning, carrier and protective agent adding, extruding, rolling and pelletizing, drying and the like. The feeding high temperature-resistant microcapsule yeast probiotics preparation prepared by the method disclosed by the invention is more uniform in particle size and good in storage stability, has better heat resistance, can tolerate processing conditions such as high temperature and high humidity in a feed pelletizing process, has a higher rate of survival. The rate of survival is kept over 90%, and viable count of probiotics is over 5*10<9>cfu/g.

Description

technical field [0001] The invention belongs to the field of feed additives and relates to a probiotic, in particular to a high-temperature-resistant microcapsule yeast probiotic preparation for feeding and a preparation method and application thereof. Background technique [0002] Since the 1950s, with the widespread use of antibiotics as feed additives, while feed antibiotics promote the growth of livestock and poultry, their disadvantages have become increasingly apparent, such as causing imbalance of normal flora in the gastrointestinal tract of animals, drug resistance, and drug residues. And reduce the quality of animal products, etc., thus causing potential harm to human health. These issues have attracted widespread attention around the world. In 2002, the World Health Organization proposed a global principle to reduce the use of antibiotics in food animals. The European Union, Japan and South Korea banned the use of antibiotics in feed in 2006 and 2008, respectivel...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12N11/12C12N11/10C12N11/08A23K1/16A23K1/00C12R1/865A23K10/18
Inventor 王志林蒋宗勇胡友军郑春田
Owner 翁源县新南都饲料科技有限公司
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