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Feeding high temperature-resistant microcapsule yeast probiotics preparation as well as preparation method and application thereof

A probiotic preparation, high temperature resistant technology, applied in the field of feed additives, can solve the problems of inactivation, enterococcus inactivation, loss of live yeast cells, etc., and achieve high survival rate, good heat resistance, and good storage stability

Active Publication Date: 2014-08-27
翁源县新南都饲料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the domestic microecological preparations are freeze-dried powders. After adding microecological powders during the processing of pellet feed and extruded feed, 10% to 30% of spores can be inactivated under high temperature and high humidity conditions, and the inactivation of enterococci can reach More than 90%, the lactobacillus is almost completely killed, and the living cell loss of yeast is also more than 90%

Method used

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  • Feeding high temperature-resistant microcapsule yeast probiotics preparation as well as preparation method and application thereof
  • Feeding high temperature-resistant microcapsule yeast probiotics preparation as well as preparation method and application thereof
  • Feeding high temperature-resistant microcapsule yeast probiotics preparation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A probiotic strain of the genus Saccharomyces was isolated from the gut contents of healthy pigs using modern microbial isolation techniques. The separation steps are as follows: under aseptic conditions, digesta samples were collected from the intestines of 14-day-old healthy piglets just slaughtered, placed in sterile glass bottles, transferred to the laboratory under ice bath conditions, and then a little food samples were taken under sterile conditions. Inoculate the millet sample into YPD medium, shake and culture at 30°C and 200rpm for 36 hours, spread the YPD culture plate after dilution, observe the colony morphology and combine with ordinary microscope inspection, pick a single colony and separate it, and screen to obtain a batch of yeast cells Strains, the growth performance of each strain was compared by shaking flask culture, and a yeast strain with excellent traits was screened, and then identified by 18S rDNA molecules. The 18S rDNA sequence of the strain w...

Embodiment 2

[0055] Embodiment 2 Preparation of a kind of high temperature resistant microcapsule yeast probiotic preparation for feeding

[0056] (1) Preparation of Saccharomyces cerevisiae mud

[0057] Inoculate Saccharomyces cerevisiae strains into wort liquid medium (malt extract powder 130g / L, autoclave at 121°C for 20 minutes and save for later use), pH 5.0, culture temperature is 30°C, shake culture for 24 hours to obtain liquid seeds For the culture solution, inoculate the liquid seed culture solution into 20L fermentation medium at a ratio of 5% (v / v), pH 5.5, temperature 30°C, supplement appropriate carbon source, nitrogen source and phosphorus source by fed-batch method, and cultivate for 36 hours , to obtain Saccharomyces cerevisiae milk, add 2.5g / L NaCl, 4g / L MgCl to the yeast milk 2 ·6H 2 After 0 and 1.5g / L Tween-80, adopt vacuum filtration to obtain Saccharomyces cerevisiae mud;

[0058] The steps of supplementing the appropriate carbon source, nitrogen source and phospho...

Embodiment 3

[0067] Example 3 Preparation of a high-temperature-resistant granule preparation for Saccharomyces cerevisiae feed

[0068] (1) Preparation of Saccharomyces cerevisiae mud

[0069] Inoculate Saccharomyces cerevisiae strains into wort liquid medium (malt extract powder 130g / L, autoclave at 121°C for 20 minutes and store for later use), pH 5.0, culture temperature is 30°C, shake culture for 36 hours to obtain liquid seeds For the culture solution, inoculate the liquid seed culture solution into 20L fermentation medium at a ratio of 10% (v / v), pH 5.0, temperature 30°C, supplement appropriate carbon source, nitrogen source and phosphorus source by fed-batch method, and cultivate 24h, obtain Saccharomyces cerevisiae milk, add 2.5g / L NaCl, 4g / L MgCl to the yeast milk 2 ·6H 2 After 0 and 1.5g / L Tween-80, adopt vacuum filtration to obtain Saccharomyces cerevisiae mud;

[0070] The steps of supplementing the appropriate carbon source, nitrogen source and phosphorus source in the fed...

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Abstract

The invention discloses a feeding high temperature-resistant microcapsule yeast probiotics preparation as well as a preparation method and application thereof, and belongs to the technical field of feed additives. The feeding high temperature-resistant microcapsule yeast probiotics preparation is prepared by the steps such as fermenting, centrifuging, cleaning, carrier and protective agent adding, extruding, rolling and pelletizing, drying and the like. The feeding high temperature-resistant microcapsule yeast probiotics preparation prepared by the method disclosed by the invention is more uniform in particle size and good in storage stability, has better heat resistance, can tolerate processing conditions such as high temperature and high humidity in a feed pelletizing process, has a higher rate of survival. The rate of survival is kept over 90%, and viable count of probiotics is over 5*10<9>cfu / g.

Description

technical field [0001] The invention belongs to the field of feed additives and relates to a probiotic, in particular to a high-temperature-resistant microcapsule yeast probiotic preparation for feeding and a preparation method and application thereof. Background technique [0002] Since the 1950s, with the widespread use of antibiotics as feed additives, while feed antibiotics promote the growth of livestock and poultry, their disadvantages have become increasingly apparent, such as causing imbalance of normal flora in the gastrointestinal tract of animals, drug resistance, and drug residues. And reduce the quality of animal products, etc., thus causing potential harm to human health. These issues have attracted widespread attention around the world. In 2002, the World Health Organization proposed a global principle to reduce the use of antibiotics in food animals. The European Union, Japan and South Korea banned the use of antibiotics in feed in 2006 and 2008, respectivel...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12N11/12C12N11/10C12N11/08A23K1/16A23K1/00C12R1/865A23K10/18
Inventor 王志林蒋宗勇胡友军郑春田
Owner 翁源县新南都饲料科技有限公司
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