Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation of a bioactive peptide from shiitake mushroom

A technology of bioactive peptides and shiitake mushrooms, applied in the fields of peptides, depsipeptides, food science, etc., to achieve the effect of improving metabolic rate, increasing the activation rate of ADH and ALDH, enhancing biological absorption and sobering effect

Active Publication Date: 2017-12-29
BEIJING FORESTRY UNIVERSITY
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No further separation of biologically active peptides from shiitake mushrooms to study their biological activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of a bioactive peptide from shiitake mushroom
  • Preparation of a bioactive peptide from shiitake mushroom
  • Preparation of a bioactive peptide from shiitake mushroom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Dissolution: Weigh 5.0g of shiitake mushroom powder and put it in the reactor, add 45g of distilled water to dissolve and fully stir to obtain the shiitake mushroom aqueous solution;

[0030] Wall breaking: Ultrasonic crushing for 20 minutes, adding 500 international units of cellulase, adjusting the pH to 4.5, heating to 45°C, and hydrolyzing for 60 minutes;

[0031] Precipitation: Cool to room temperature, add sodium hydroxide to adjust the pH to 10, extract in a cold water bath at 10°C for 30 minutes, centrifuge, and take the supernatant; concentrate under reduced pressure in a vacuum concentration equipment at a temperature of 44°C for 90 minutes; add Water ethanol to a concentration of 83% precipitation, ice water bath. Stand at 4°C for 60 minutes. Discard the supernatant by centrifugation, collect the precipitate, and dry at 40°C to obtain the mushroom protein:

[0032] Enzymolysis: Dissolve mushroom protein in 50 times distilled water, the unit is g. Adjust th...

Embodiment 2

[0035] Dissolving: Weigh 10.0kg of shiitake mushroom powder and place it in a reactor, add 100kg of distilled water to dissolve and fully stir to obtain an aqueous solution of shiitake mushrooms;

[0036] Wall breaking: Ultrasonic crushing for 20 minutes, adding 2 million international units of cellulase, adjusting the pH to 5.0, heating to 50°C, and hydrolyzing for 90 minutes;

[0037] Precipitation: Cool to room temperature, add sodium hydroxide to adjust the pH to about 10, extract in a cold water bath at 8°C for 40 minutes, centrifuge, and take the supernatant; concentrate under reduced pressure in a vacuum concentration equipment at a temperature of 45°C for 120 minutes; add Absolute ethanol to a concentration of 85% for precipitation, ice water bath. Stand at 4°C for 90 minutes. Discard the supernatant by centrifugation, collect the precipitate, and dry at 45°C to obtain the mushroom protein;

[0038] Enzymolysis: Dissolve the mushroom protein in 60 times distilled wat...

Embodiment 3

[0041] The optimal experimental conditions for papain, alkaline protease, Protamex protease, and trypsin to hydrolyze protein were obtained by consulting the literature. The above four enzymes were used to hydrolyze the same amount of shiitake mushroom powder, umbrella powder, and whole powder, and the formaldehyde titration method was used to detect The degree of hydrolysis of each hydrolyzate, the results are shown in Table 1.

[0042] Table 1 Comparison of hydrolysis degree of four kinds of enzymes to shiitake mushroom powder, whole powder and foot powder

[0043]

[0044] Note: Protamex protease was purchased from Novozymes.

[0045] As can be seen from Table 1, the present invention intends to take mushroom foot powder as the experimental raw material. As can be seen from the experimental results, taking mushroom as the substrate and the order of different enzymatic hydrolysis degrees are: alkaline protease>Protamex protease>trypsin> Papain, according to the results o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing biologically active peptides of shiitake mushrooms. The specific steps are as follows: 1) adding 6 to 12 times the mass of distilled water to the shiitake mushroom powder to dissolve; 2) ultrasonic crushing treatment, adding 100,000 to 200,000 international Unit cellulase, adjust pH to 4.0-5.0, temperature 40-50°C, hydrolyze for 60-120min; 3) Cool to room temperature, adjust pH to 9.5-10.5 with sodium hydroxide, extract in cold water bath at 5-15°C for 20-40min, The supernatant was collected by centrifugation, concentrated under reduced pressure, and added absolute ethanol until the concentration was 83%-85%. Let stand at 4°C for 60-90 minutes. Centrifuge, collect the precipitate, and dry to obtain the shiitake mushroom protein; 4) Dissolve the shiitake mushroom protein in 40-60 times the mass of distilled water, adjust the pH to 6.0-7.0, add 0.2%-0.4% compound protease for hydrolysis, and the temperature is 45-55°C, Hydrolyze for 150-200 minutes. Inactivate the enzyme, centrifuge, and collect the filtrate; 5) use a 0.22 μm filter membrane to micro-filter and ultra-filter, collect peptides with a relative molecular mass of 5-10 KD, and vacuum freeze-dry to obtain a brown mushroom bioactive peptide freeze-dried powder. The biologically active peptide of shiitake mushroom prepared by the invention has better in vitro antioxidant activity and sobering activity.

Description

technical field [0001] The invention relates to a method for preparing bioactive peptides from shiitake mushrooms, which belongs to the field of modern food processing biotechnology. Background technique [0002] Lentinus edodes belongs to Basidiomycetes, Agaricales, Tricholomataceae, Lent inus, scientific name Lentinus edodes, is a precious edible fungus with long reputation in my country. According to analysis, the edible part of dried shiitake mushrooms accounts for 72%, and every 100g edible part contains 13g of water, 1.8g of fat, 54g of carbohydrate, 7.8g of crude fiber, 4.9g of ash, 124mg of calcium, 415mg of phosphorus, 25.3mg of iron, and 10.07mg of vitamin B , Vitamin B2 1.13mg, Niacin 18.9mg. The amino acid in shiitake mushroom is very rich, there are 20 kinds of amino acids that constitute human protein, there are 18 kinds of shiitake mushrooms, including 8 kinds of essential amino acids for human body, and its nutritional value is 4 times that of beef. Mushroo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34C07K14/375A23L33/18A61P39/06A61P25/32
Inventor 任迪峰杨宇迪程湛匡绮蓉鲁军任剑
Owner BEIJING FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com