Microbial preparation for improvement of freshwater aquaculture water body

A technology for microbial preparation and water improvement, which is applied in the fields of microorganism-based methods, microorganisms, biological water/sewage treatment, etc. The effect of reducing and increasing the amount of dissolved oxygen

Inactive Publication Date: 2014-09-17
SHANGHAI WEIMING BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, the water used for aquaculture and the environment have been seriously polluted, and the situation of aquaculture drugs has become more and more serious, leading to drug resistance of pathogenic bacteria, and the residual amount of aquaculture biological drugs exceeds the standard, bringing some diseases to consumers

Method used

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  • Microbial preparation for improvement of freshwater aquaculture water body
  • Microbial preparation for improvement of freshwater aquaculture water body
  • Microbial preparation for improvement of freshwater aquaculture water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Raw material composition:

[0053]

[0054] Preparation:

[0055] step 1:

[0056] Bacillus subtilis and Bacillus amyloliquefaciens were respectively inoculated in the first culture medium under aseptic conditions, 30 DEG C-35 DEG C, single bacterial species was cultivated for 48 hours, obtained Bacillus subtilis and Bacillus amyloliquefaciens seed culture solution, which The number of live bacteria is greater than 5.0×10 9 cfu / ml. Among them, the first medium is composed of molasses 1-1.2wt%, peptone 0.4-0.6wt%, yeast extract 1-1.2wt%, calcium chloride 0.1-0.15wt%, sodium chloride 0.2-0.25wt%, iron sulfate 30 -35wt%, magnesium sulfate 0.02-0.03wt%, growth factor solution 1-1.2wt% (including vitamin H2-2.3wt%, vitamin B12-15wt% aqueous solution) and the rest of water, the pH value is 6.5-6.8 .

[0057] Inoculate Rhodopseudomonas palustris in the second culture medium under aseptic conditions, under the conditions of 4500-10000lx light intensity and 29°C-33°C tem...

Embodiment 2

[0068] Raw material composition:

[0069]

[0070] Preparation:

[0071] step 1:

[0072] According to the method described in Example 1, the seed culture liquid of Bacillus subtilis, the seed culture liquid of Bacillus amyloliquefaciens and the seed culture liquid of Rhodopseudomonas palustris were provided.

[0073] Step 2,

[0074] According to the method described in Example 1, a high-concentration concentrated bacterial solution was obtained as a mother solution.

[0075] Step 3,

[0076] Move the concentrated bacterial liquid into the matrix composed of zeolite powder + calcium carbonate for feed + cornstarch + molasses and mix. The mixed fluid is granulated on a swing machine, and then boiled and dried. The mass percentage of moisture after drying is 5-7%.

[0077] Wherein, based on the total weight of the matrix (carrier and nutrients), the matrix is ​​composed of 75wt% zeolite powder, 10wt% calcium carbonate, 8wt% cornstarch, and 7wt% molasses.

[0078] Step 4...

Embodiment 3

[0081] Raw material composition:

[0082]

[0083]

[0084] Preparation:

[0085] step 1:

[0086] According to the method described in Example 1, the seed culture liquid of Bacillus subtilis, the seed culture liquid of Bacillus amyloliquefaciens and the seed culture liquid of Rhodopseudomonas palustris were provided.

[0087] Step 2,

[0088] According to the method described in Example 1, a high-concentration concentrated bacterial solution was obtained as a mother solution.

[0089] Step 3,

[0090] Move the concentrated bacterial liquid into the matrix composed of zeolite powder + calcium carbonate for feed + cornstarch + molasses and mix. The mixed fluid is granulated on a swing machine, and then boiled and dried. The mass percentage of moisture after drying is 5-7%.

[0091] Wherein, based on the total weight of the matrix (carrier and nutrients), the matrix is ​​composed of 65wt% zeolite powder, 15wt% calcium carbonate, 5wt% cornstarch and 5wt% molasses.

[0...

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Abstract

The invention provides a microbial preparation for improvement of a freshwater aquaculture water body. Raw materials of the microbial preparation comprise, by weight, 15-40% of bacillus subtilis, 15-40% of bacillus amyloliquefaciens and 10-20% of rhodopseudomonas palustris. The invention also provides a method for preparing the above microbial preparation, comprising the following steps: successively inoculating bacillus subtilis, bacillus amyloliquefaciens and rhodopseudomonas palustris to a medium, fermenting and mixing a fermented liquid obtained with a carrier and nutrients so as to obtain the microbial preparation. By the use of the microbial preparation provided by the invention, water quality of an aquaculture water body can obviously be improved, and meat quality of farmed animals also can be raised.

Description

technical field [0001] The invention relates to a microbial mixture, in particular to a microbial preparation which can be used for improving fresh water bodies. Background technique [0002] China is the world's largest aquaculture country. According to statistics, China's inland aquaculture water area is 27.34 million hectares, seawater aquaculture area is 4.38 million hectares, and rice field fish farming area is 1.52 million hectares in 2001. Especially in the water towns in southern China, aquaculture plays an important role in agricultural production. Many areas regard aquaculture as an important way for local farmers to get rich. Aquaculture has gradually become one of the pillar industries in aquaculture agriculture. [0003] At the same time, China is also one of the countries with the most serious pollution of aquaculture water. Since ancient times, there has been a saying in China that "raise fish first by raising water". The quality of water is directly related ...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20C12R1/07C12R1/125C12R1/38
Inventor 刘杨周文明
Owner SHANGHAI WEIMING BIOTECH
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