Production process of fermented cottonseed meal
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preparation example Construction
[0030] (1) Preparation of the bacteria solution:
[0031] Add the following raw materials: barley malt, peptone, agar, sodium citrate, potassium dihydrogen phosphate, magnesium sulfate, sodium bicarbonate and water into the batching tank, the mass ratio of the raw materials is barley malt: peptone: agar: citric acid Sodium: potassium dihydrogen phosphate: magnesium sulfate: sodium bicarbonate: water = 2.0-3.0: 1.5-1.8: 2.0-3.0: 0.5-1.0: 0.3-0.4: 0.015-0.020: 0.5-0.8: 180-220, heated to After boiling, adjust the pH value to 4.5-5.5 and then transfer to the saccharification tank, add 9×10 5 U-10×10 5 U glucoamylase, heat at 55-65°C for 3-5 hours to obtain a saccharification liquid, transfer the obtained saccharification liquid to a fluid heater to raise the temperature to 95-100°C, keep it warm for 0.5 hours, and then cool it down to 35°C within 5-10 seconds -45°C, get the breeding medium, and add it to four chemostats;
[0032] The strains of lactic acid bacteria, bifidobac...
Embodiment 1
[0050] This embodiment includes the following steps:
[0051] (1) Preparation of the bacteria solution:
[0052] Add 2.5kg of barley malt, 1.7kg of peptone, 2.5kg of agar, 0.8kg of sodium citrate, 0.3kg of potassium dihydrogen phosphate, 0.018kg of magnesium sulfate, 0.7kg of sodium bicarbonate and 190kg of water into the batching tank, heat until boiling, and adjust After the pH value reached 4.8, it was transferred to the saccharification tank, and 10×10 5 U glucoamylase, heat at 65°C for 3 hours to obtain a saccharification solution, transfer the obtained saccharification solution to a fluid heater to raise the temperature to 95-100°C, keep it warm for 0.5 hours, and then cool down to 35°C within 10 seconds to obtain a breeding medium , and added to four chemostats;
[0053] The strains of lactic acid bacteria, bifidobacteria, bacillus subtilis and candida utilis cultured in the laboratory were respectively inserted into four chemostats with an arrow inoculator, and each ...
Embodiment 2
[0058] This embodiment includes the following steps:
[0059] (1) Preparation of the bacteria solution:
[0060] Add 2.4kg of barley malt, 1.6kg of peptone, 2.4kg of agar, 0.8kg of sodium citrate, 0.36kg of potassium dihydrogen phosphate, 0.019kg of magnesium sulfate, 0.6kg of sodium bicarbonate and 200kg of water into the batching tank, heat until boiling, and adjust After the pH value reached 5.0, it was transferred to the saccharification tank, and 9.6×10 5 The glucoamylase of U was incubated at 60°C for 4 hours to obtain a saccharification solution, and the obtained saccharification solution was transferred to a fluid heater to raise the temperature to 95°C, keep it warm for 0.5 hours, and then cool down to 40°C within 8 seconds to obtain a breeding medium, and into four chemostats;
[0061] The strains of lactic acid bacteria, bifidobacteria, bacillus subtilis and candida utilis cultured in the laboratory were respectively inserted into four chemostats with an arrow typ...
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