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Method for extracting and purifying triterpenoids from apple pomace and application of triterpenoids

A purification method and apple pomace technology, which is applied in the fields of medicine and health food, and triterpenes purification, can solve the problems of less research and achieve the purpose of increasing GSH content, strong total antioxidant capacity, reducing MDA level and IL-6 content Effect

Inactive Publication Date: 2015-04-22
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CCl 4 The resulting acute liver injury is similar to human liver disease in some aspects of morphology and pathophysiology, and CCl 4 The mechanism of acute liver injury is closely related to the conduction of free radicals. Because triterpenoids have the functions of protecting the liver, anti-tumor, anti-bacteria and anti-inflammation, anti-virus, improving immunity, lowering blood sugar and blood fat, they are widely used as health food , raw materials for medicine and cosmetics, oleanolic acid and ursolic acid in triterpenes have been proved to have obvious hepatoprotective effects, and there are few studies on triterpenes in apple pomace

Method used

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  • Method for extracting and purifying triterpenoids from apple pomace and application of triterpenoids
  • Method for extracting and purifying triterpenoids from apple pomace and application of triterpenoids
  • Method for extracting and purifying triterpenoids from apple pomace and application of triterpenoids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Preparation and content determination of apple pomace triterpene crude extract and purified product

[0026] 1. Preparation of apple pomace triterpene crude extract and purified product

[0027] (1) Rough extraction: Mix 100-120 mesh dry apple pomace powder with a solid-liquid ratio (m:v) of 1:18 and ethanol solution, the volume fraction of ethanol solution is 95%, and place it in an ultrasonic device for repeated extraction 3 times , the temperature is 40°C, the extraction time is 20min, and then filtered, the filtrates extracted three times are combined, centrifuged at 4000rpm for 10min, and the supernatant is concentrated under reduced pressure at 60°C to obtain the extract of apple pomace triterpenoids;

[0028] (2) Impurity removal: Mix the extract of apple pomace with petroleum ether according to the ratio of material to liquid (m:v) of 1:35, and place it in an ultrasonic equipment for repeated washing 6 times. The temperature of ultrasonic washing is 50...

Embodiment 2

[0040] Example 2 Determination of free radical scavenging and total antioxidant capacity of apple pomace triterpene crude extract and purified product

[0041] 1 Preparation of crude extract and purified triterpenoids from apple pomace

[0042] The preparation of apple pomace triterpene crude extract and purified product is the same as in Example 1.

[0043] 2 Determination of the ability to scavenge DPPH free radicals

[0044] (1) Preparation of crude extract / purified sample solution

[0045] Accurately weigh 100 mg of apple pomace triterpene crude extract and 100 mg of purified product, place them in 50 mL volumetric flasks, dissolve them with 95% ethanol and make up to volume to obtain a sample stock solution with a mass concentration of 2.0 mg / mL, and take the stock solution 1.25, 2.50, 3.75, 5.00, 6.25, 7.50, 8.75, 10.00mL in a 10mL volumetric flask, after constant volume with 95% ethanol solution, the mass concentration is 0.25, 0.50, 0.75, 1.00, 1.25, 1.50, 1.75, 2.0...

Embodiment 3

[0080] Example 3 Protective effect of purified apple pomace triterpenoids on acute liver injury in mice induced by carbon tetrachloride

[0081] 1 Preparation of purified apple pomace triterpenes

[0082] The preparation of apple pomace triterpene purified is the same as in Example 1.

[0083] 2 animals:

[0084] SPF grade male BALB / c mice (18-22g). The feeding conditions were temperature 23±2°C, humidity (60±5)%, food and water were free to eat and drink during the adaptation period, and the feed was ordinary feed.

[0085] 3 Grouping and administration of animals

[0086] The mice were randomly divided into 6 groups, 8 in each group, respectively: blank control group, model control group, silymarin positive group (30mg / kg), apple pomace triterpene purified high, medium and low (60, 30, 15mg / kg) dose group, the blank control group and the model group were intragastrically administered 5% Tween-80 prepared by normal saline daily, and each administration group was intragast...

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Abstract

The invention relates to the technical field of purification of triterpenoids, medicine and health foods and particularly relates to a method for extracting and purifying triterpenoids from apple pomace and application of the triterpenoids. The method comprises the following steps of mixing dried 100-120-mesh apple pomace serving as a raw material and 95% ethanol solution at a ratio of the material to liquid being 1:18, carrying out ultrasonic extraction and drying filtrate to obtain an apple pomace triterpenoid extract; mixing the extract and petroleum ether according to a ratio of the material to liquid being (1:30)-(1:40), repeatedly washing with ultrasonic waves for 4-6 times at 40-60 DEG C and 40-60 minutes each time and drying filter residue to obtain an apple pomace triterpenoid crude extract; and finally adsorbing and eluting triterpenoids in apple pomace by macroporous resin to obtain an eluent, concentrating and drying the eluent to obtain an apple pomace triterpenoid purified product. The invention further discloses main active ingredients (ursolic acid and oleanolic acid) in the apple pomace triterpenoid crude extract and the purified product and two extracts have the characteristic of scavenging free radical and antioxidant capacity and have significant improving and protecting effect on acute liver injury.

Description

1. Technical field: [0001] The invention relates to the technical fields of purification of triterpenes, medicine and health food, in particular to an extraction and purification method for extracting triterpenes from apple pomace and its application. 2. Background technology: [0002] Apple pomace is the leftovers left over from the production of concentrated juice, canned food, jam, etc. of apples. It is mainly composed of peel, pulp, seeds, and fruit stems. Generally, for every 1,000 tons of apples processed, about 300 tons of fresh pomace can be discharged, and about 75 tons of dried pomace can be obtained after drying. At present, except a small amount of apple pomace is used for feed and deep processing, most of it is abandoned. [0003] CCl 4 Cytochrome P in liver microsomal 450 Enzyme activation produces active free radical CCl 3 , CCl 3 Further react with oxygen to form more active free radicals CCl 3 COO·, these free radicals can covalently bond with macromo...

Claims

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Application Information

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IPC IPC(8): A61K36/73A23L1/29A61K8/97A61P39/06A61P1/16A61Q19/08A23L33/00
Inventor 任亚梅张爽
Owner NORTHWEST A & F UNIV
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