Method for extracting and purifying triterpenoids from apple pomace and application of triterpenoids

A purification method and apple pomace technology, which is applied in the fields of medicine and health food, and triterpenes purification, can solve the problems of less research and achieve the purpose of increasing GSH content, strong total antioxidant capacity, reducing MDA level and IL-6 content Effect

Inactive Publication Date: 2015-04-22
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CCl 4 The resulting acute liver injury is similar to human liver disease in some aspects of morphology and pathophysiology, and CCl 4 The mechanism of acute liver injury is closely related to the conduction of free radicals. Because triterpenoids have the functions of protecting the liver, anti-tumor, anti-bacteria and ant

Method used

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  • Method for extracting and purifying triterpenoids from apple pomace and application of triterpenoids
  • Method for extracting and purifying triterpenoids from apple pomace and application of triterpenoids
  • Method for extracting and purifying triterpenoids from apple pomace and application of triterpenoids

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1 Preparation and content determination of crude and purified triterpenoids of apple pomace

[0026] 1. Preparation of crude extract and purified product of triterpene from apple pomace

[0027] (1) Crude extraction: Mix 100-120 mesh dry apple pomace powder with a material-to-liquid ratio (m:v) of 1:18 with ethanol solution, the volume fraction of ethanol solution is 95%, and repeat extraction 3 times in an ultrasonic device , The temperature is 40℃, the extraction time is 20min, then filter, combine the filtrates of the three extractions, centrifuge at 4000rpm for 10min, the supernatant is concentrated under reduced pressure at 60℃ to obtain an extract of apple pomace triterpene;

[0028] (2) Impurity removal: The apple pomace extract is mixed with petroleum ether according to the material-to-liquid ratio (m:v) 1:35, and it is placed in an ultrasonic device for repeated washing 6 times. The temperature of ultrasonic washing is 50℃, and each washing The time is 50 min...

Example Embodiment

[0040] Example 2 Determination of Free Radical Scavenging and Total Antioxidant Ability of Crude Extract and Purified Triterpene of Apple Pomace

[0041] 1 Preparation of crude extract and purified product of apple pomace triterpene

[0042] The preparation of the crude extract and purified product of apple pomace triterpene is the same as in Example 1.

[0043] 2 Determination of the ability to scavenge DPPH free radicals

[0044] (1) Preparation of crude extract / purified product sample solution

[0045] Precisely weigh 100mg each of the crude extract and purified triterpene of apple pomace, put them in a 50mL volumetric flask, dissolve them with 95% ethanol and dilute the volume to obtain a sample stock solution with a mass concentration of 2.0mg / mL. Take the stock solutions separately 1.25, 2.50, 3.75, 5.00, 6.25, 7.50, 8.75, 10.00mL in a 10mL volumetric flask, after constant volume with 95% ethanol solution, the mass concentration is 0.25, 0.50, 0.75, 1.00, 1.25, 1.50, 1.75, 2.00m...

Example Embodiment

[0080] Example 3 Protective effect of purified triterpenes from apple pomace on carbon tetrachloride-induced acute liver injury in mice

[0081] 1 Preparation of purified triterpene from apple pomace

[0082] The preparation of the purified triterpene of apple pomace was the same as in Example 1.

[0083] 2 Animals:

[0084] SPF male BALB / c mice (18-22g). The feeding conditions are temperature 23±2°C, humidity (60±5)%, free eating and drinking during the adaptation period, and normal feed.

[0085] 3 Animal grouping and administration

[0086] The mice were randomly divided into 6 groups, 8 in each group, namely: blank control group, model control group, silymarin-positive group (30mg / kg), high, medium and low purified triterpene from apple pomace (60, 30, 30 mg / kg) 15mg / kg) dose group, blank control group and model group were intragastrically administered 5% Tween-80 prepared with normal saline daily, and each administration group was intragastrically administered corresponding dose o...

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Abstract

The invention relates to the technical field of purification of triterpenoids, medicine and health foods and particularly relates to a method for extracting and purifying triterpenoids from apple pomace and application of the triterpenoids. The method comprises the following steps of mixing dried 100-120-mesh apple pomace serving as a raw material and 95% ethanol solution at a ratio of the material to liquid being 1:18, carrying out ultrasonic extraction and drying filtrate to obtain an apple pomace triterpenoid extract; mixing the extract and petroleum ether according to a ratio of the material to liquid being (1:30)-(1:40), repeatedly washing with ultrasonic waves for 4-6 times at 40-60 DEG C and 40-60 minutes each time and drying filter residue to obtain an apple pomace triterpenoid crude extract; and finally adsorbing and eluting triterpenoids in apple pomace by macroporous resin to obtain an eluent, concentrating and drying the eluent to obtain an apple pomace triterpenoid purified product. The invention further discloses main active ingredients (ursolic acid and oleanolic acid) in the apple pomace triterpenoid crude extract and the purified product and two extracts have the characteristic of scavenging free radical and antioxidant capacity and have significant improving and protecting effect on acute liver injury.

Description

1. Technical field: [0001] The invention relates to the technical fields of purification of triterpenes, medicine and health food, in particular to an extraction and purification method for extracting triterpenes from apple pomace and its application. 2. Background technology: [0002] Apple pomace is the leftovers left over from the production of concentrated juice, canned food, jam, etc. of apples. It is mainly composed of peel, pulp, seeds, and fruit stems. Generally, for every 1,000 tons of apples processed, about 300 tons of fresh pomace can be discharged, and about 75 tons of dried pomace can be obtained after drying. At present, except a small amount of apple pomace is used for feed and deep processing, most of it is abandoned. [0003] CCl 4 Cytochrome P in liver microsomal 450 Enzyme activation produces active free radical CCl 3 , CCl 3 Further react with oxygen to form more active free radicals CCl 3 COO·, these free radicals can covalently bond with macromo...

Claims

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Application Information

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IPC IPC(8): A61K36/73A23L1/29A61K8/97A61P39/06A61P1/16A61Q19/08A23L33/00
Inventor 任亚梅张爽
Owner NORTHWEST A & F UNIV
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