Soft tissue separation preparation prepared from elastin-like polypeptide and application of soft tissue separation preparation

An elastin-like and elastin-like technology, which is applied in the fields of peptide/protein components, drug combinations, and medical preparations containing active ingredients, etc., can solve the problem of short maintenance time, high repeated injection rate, and inability to effectively reduce the complications of endoscopic treatment. Incidence and other problems, to achieve the effect of increasing separation distance, safe and effective operation, and reducing operation time

Active Publication Date: 2015-06-24
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problems in the prior art that the traditional soft tissue separation preparations have a short maintenance time after injection, a high rate of repeated injections, cannot

Method used

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  • Soft tissue separation preparation prepared from elastin-like polypeptide and application of soft tissue separation preparation
  • Soft tissue separation preparation prepared from elastin-like polypeptide and application of soft tissue separation preparation
  • Soft tissue separation preparation prepared from elastin-like polypeptide and application of soft tissue separation preparation

Examples

Experimental program
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preparation example Construction

[0029] 1. Preparation of elastin-like polypeptides

[0030] 1. The preparation method of elastin-like polypeptide:

[0031] ① Transform the vector expressing the elastin-like polypeptide into the expression strain of Escherichia coli;

[0032] ② The BL21 engineered bacteria containing the pET-22b-ELPs recombinant plasmid were inoculated into TB medium containing 100 μg / mL ampicillin at an inoculum size of 1:100 (12 g peptone per liter of TB medium, 24 g yeast extract extract, 2.31 g dipotassium hydrogen phosphate, 12.54 g potassium dihydrogen phosphate, 4 mL glycerol), cultured at 250 r / min at 37°C for 24 h;

[0033] ③ Centrifuge at 4 ℃, 4 000 r / min for 15 min, harvest the cells, and use pre-cooled PBS buffer solution (137 mmol / L NaCl, 2.7 mmol / L KCl, 4.2 mmol / L NaH2PO4, 1.4 mmol / L KH2PO4, pH 7.3) Resuspend the bacterial cells, and then put them in an ice bath to sonicate (sonicate for 2 seconds, with an interval of 3 seconds, for a total of 5 minutes);

[0034] ④ The elast...

Embodiment 1

[0048] Example 1: Study on the effectiveness and safety of soft tissue separation preparation prepared by elastin-like polypeptide in rat gastric submucosal dissection

[0049] 1.1 Experimental method:

[0050] 54 male SD rats were randomly divided into three parts (n=18). For each experiment, one of the three groups was randomly selected to carry out the following experiments at the same time, and the experiment was repeated. After fasting for 24 hours, they were weighed and anesthetized by intraperitoneal injection of 5% chloral hydrate (0.7ml / 100g). Rats were placed in a supine position, and their limbs were fixed on a sterile test bench. The abdomen was cut open, and the gauze pad was used to expose the rat stomach. The iris scissors were used to cut the appetizing tissue along the greater curvature of the stomach, and the stomach wall was opened to expose the gastric mucosa and remove residues. During the whole experimental operation, the breathing and heart rate of the...

Embodiment 2

[0061] Example 2: Study on the effectiveness and safety of soft tissue separation preparation prepared by elastin-like polypeptide in endoscopic mucosal dissection

[0062] 2.1 Experimental method:

[0063] Piglets (35.1±3.5kg) were fasted for 24 hours, prepared the skin on the legs of the piglets, took the left side lying position, intramuscularly injected ketamine (20mg / kg), intubated and connected to a ventilator, and intravenously induced with propofol. Maintain anesthesia. At the same time, turn on the endoscope host, adjust the workstation and recorder, install the gastroscope, connect the suction device, connect the electric cutting device, and prepare disposable injection needles and syringes. Use the endoscope to enter the endoscope through the cavity, slowly supply a small amount of air, and reach the stomach; use the argon knife to mark the APC, with a range of about 3.0cm; insert a disposable injection needle from the biopsy hole, and inject 0.5% methylene blue on...

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Abstract

The invention discloses a soft tissue separation preparation prepared from elastin-like polypeptide and an application of the soft tissue separation preparation and belongs to a medicinal preparation product and application of the elastin-like polypeptide. The soft tissue separation preparation prepared from elastin-like polypeptide is liquid pharmaceutical preparation which is obtained by dissolving elastin-like polypeptide air dried flocculent solid substances in phosphate buffer solution and has the final concentration of 500-1000mu M; and the application of the soft tissue separation preparation comprises the step of enabling a gastrointestinal tract submucous layer to be filled with the liquid pharmaceutical preparation, and an elastin-like polypeptide phase is changed to be gelatinous, so that a gastrointestinal mucosa layer is fully separated from an muscularis propria. After the filling agent is injected, gastrointestinal tract soft tissue separation distance is obviously increased, separation duration time is prolonged, multiple injection does not need to be carried out; the soft tissue separation preparation has good biocompatibility; and the soft tissue separation preparation has degradable characteristic, and worry of residual foreign matters is eliminated, so that the soft tissue separation preparation prepared from elastin-like polypeptide is applicable to soft tissue isolation during treatment under a gastroendoscope, lesion tissues of digestive tracts can be conveniently cut off, and operation is safe and effective.

Description

technical field [0001] The invention relates to soft tissue separation preparations and applications prepared from elastin-like polypeptides, and belongs to medical preparations and uses of elastin-like polypeptides. technical background [0002] In the endoscopic treatment of the digestive tract, endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) are the standard minimally invasive treatments widely used to remove precancerous lesions and early tumors of the digestive tract. means. They can not only save patients from the pain of surgical laparotomy, but also recover tissue after stripping, conduct pathological diagnosis, guide postoperative review and further treatment, so they have gradually become the first choice for the treatment of early-stage gastrointestinal tumors. Although this endoscopic treatment has the advantages of simple method, less trauma, and reliable curative effect, its operation technique is difficult, and there are a consid...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P41/00
Inventor 刘文天刘文革王邦茂郑忠青王涛赵明星张续乾荆洋
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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