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Collagen peptide with function of promoting bone growth as well as preparation method and application thereof

A technology for bone growth and collagen peptides, applied in application, food preparation, specific peptides, etc., can solve problems such as great differences, achieve the effect of small molecular weight, high protein purity, and promote osteoblast proliferation activity

Inactive Publication Date: 2015-07-01
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The selection of protease in the process of enzymatic hydrolysis of protein is the first key element in the preparation of collagen peptides. At present, the proteases that are widely used in actual production mainly include proteases from various microbial sources, plant sources and animal sources, including alkaline protease , flavor enzyme, papain, neutral protease, trypsin, etc.; Therefore, how to identify and select suitable enzymes and cooperate with appropriate process parameters is to achieve the required modification effects on the collagen matrix raw materials used and then prepare them. The biggest difficulty in obtaining collagen peptides with specific bioactivity

Method used

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  • Collagen peptide with function of promoting bone growth as well as preparation method and application thereof
  • Collagen peptide with function of promoting bone growth as well as preparation method and application thereof
  • Collagen peptide with function of promoting bone growth as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0063] Two, embodiment 2: the preparation of collagen peptide

[0064] It mainly includes the following steps:

[0065] 1. Remove fish scales and adherent fish flesh from fresh fish skin, and wash;

[0066] 2. Collagen degradation: add 4 to 5 times the weight of the acid solution with a mass concentration of 1 to 5 g / L to the material prepared in step 1, soak evenly for 20 to 120 minutes; then heat to 55 to 60 ° C and keep stirring 10 to 60 minutes; filter to remove the filter residue, add protease to the filtrate at a rate of 6000 to 8000 U / g collagen matrix, adjust the pH to 6.5 to 7.5, and react at a temperature of 50 to 55°C for 7 to 8 hours; then raise the temperature to boiling And keep it for 1-2 minutes. The acid solution may be hydrochloric acid, acetic acid, lactic acid, or phosphoric acid.

[0067] 3. Clarification: take the filtrate. Specifically, filter cloth filtration, and / or diatomaceous earth plate and frame filtration, and / or centrifugal clarification can...

Embodiment 3

[0071] Three, embodiment 3: the preparation of collagen peptide

[0072] The technical solution of this example is basically the same as that of Example 2, except that the protease used is animal protease and flavor enzyme, and the two are used in combination at a mass ratio of 1:1.

[0073] Through the cross-test of different data, the test results of the degree of hydrolysis show that the average degree of hydrolysis reaches 14.52% by using animal protease and flavor enzyme compound enzymatic hydrolysis.

[0074] Cell experiments and amino acid composition testing show that compared with the collagen peptide powder prepared by the method of this example and the previous example, the proportion of small molecule peptides in the range of 500Da to 180Da is slightly larger than that of the previous example, and the osteoblasts proliferate and better differentiation.

Embodiment 4

[0075] Four, embodiment 4: the preparation of collagen peptide

[0076] The technical scheme of this embodiment is basically the same as that of Example 2, the difference being that the clarification step specifically adopts the following technical process: the enzymolyzate obtained in the collagen degradation step is coarsely filtered with a filter cloth while it is hot, Add 0.5~5.0g of activated carbon, then keep it warm at 40~50°C for 20~25min, and then filter through diatomaceous earth plate frame to obtain clear liquid.

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Abstract

The invention discloses a collagen peptide with the function of promoting bone growth as well as a preparation method and an application thereof. The collagen peptide is obtained from a fish skin collagen matrix through impurity removal, collagen degradation, clarification, concentration and drying. The method disclosed by the invention determines a collagen peptide for promoting bone growth by promoting bone cell proliferation and differentiation through screening and control of an enzyme condition and activity verification. The collagen peptide processed by using the method is uniform in product colour, fine in powder, good in solubility, free from a fishy smell and a peculiar smell, high in activity of promoting bone cell proliferation and differentiation, and good in application prospect in the field of food processing and the aspect of nutritious and nourishing health foods.

Description

technical field [0001] The invention relates to the technical field of protein bioutilization and processing, and more specifically relates to a collagen peptide capable of promoting bone growth and its preparation method and application. Background technique [0002] Protein is an important nutrient for humans. It is hydrolyzed into peptides or amino acids by protease through the human digestive tract, and then absorbed by the human body to synthesize new proteins. Modern studies have shown that small peptides often have better digestion and absorption characteristics than amino acids. In addition to the characteristics of high absorption and utilization (close to 100%), promoting amino acid absorption and protein synthesis, improving immunity, and promoting mineral absorption, usually It also has certain physiological activity and is very beneficial to the human body. [0003] Recently, with the continuous development of biotechnology and enzyme preparation industry, the ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/34A23L1/305
Inventor 刘小玲江虹锐姜元欣蒋智然赵谋明
Owner GUANGXI UNIV
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