Cell impedance sensor based on DFP-ITO electrode and application of cell impedance sensor

A sensor and electrode technology, which is applied in the field of simultaneous detection of cell morphology and impedance information, can solve the problems of complex process, limitation of ITO electrode preparation and promotion in cell impedance biosensors, and expensive dependence, so as to achieve good light transmission and reduce The effect of complex process and simple operation

Inactive Publication Date: 2015-08-05
AFFILIATED YONGCHUAN HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above studies report the application value of ITO electrode materials in cell impedance biosensors, the processing of ITO electrodes depends on expensive sputtering and lithography equipment, and the process is complicated, which limits the preparation and promotion of ITO electrodes in cell impedance biosensors

Method used

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  • Cell impedance sensor based on DFP-ITO electrode and application of cell impedance sensor
  • Cell impedance sensor based on DFP-ITO electrode and application of cell impedance sensor
  • Cell impedance sensor based on DFP-ITO electrode and application of cell impedance sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Photosensitive Dry Membrane Cytocompatibility Detection

[0045] The cell biocompatibility of photosensitive dry film was verified by cell adhesion experiment and cytotoxicity experiment.

[0046] (1) Cell adhesion experiment process: cut the photosensitive dry film into a square piece with an area of ​​8 mm × 8 mm, and place the photosensitive dry film in 96 holes after ultraviolet irradiation and development according to the DFP-ITO electrode processing process of the present invention. Inside the plate, disinfect with 70% (v / v) concentration of ethanol. 100 μl of A549 cells at a concentration of 2.5 × 10 5 The cells / ml were inoculated on the surface of photosensitive dry film, cultured at 37°C and 5% carbon dioxide for 24 hours to make the cells adhere to the wall, and the mitochondria of the cells were fluorescently stained with a PBS solution with a concentration of 10 μM rhodamine 123 (Sigma), and the inverted fluorescence microscope ( IX-71, Olympus) ...

Embodiment 2

[0049] Embodiment 2 Photosensitive dry film-processing of indium tin oxide electrode

[0050] The ITO conductive glass was ultrasonically cleaned with acetone, absolute ethanol, and deionized water for 15 minutes each, and dried for later use.

[0051] The schematic diagram of the structure of the DFP-ITO electrode prepared in this embodiment is as follows figure 1 shown. Photosensitive dry film-indium tin oxide electrode, comprising a conductive base layer 1 and an insulating layer 2 positioned on the conductive base layer 1, the insulating layer 2 is provided with electrode holes 3, and the electrode holes 3 are four, The conductive base layer 1 is also provided with a lead interface 4, which is connected to a power supply through the lead interface 4; the conductive base layer 1 is indium tin oxide conductive glass, and the insulating layer 2 is a photosensitive dry film.

[0052] The processing flow of DFP-ITO electrode is as follows: figure 2 As shown, it specifically...

Embodiment 3

[0056] Example 3 Cell Impedance Sensor Based on DFP-ITO Electrode

[0057] The structure diagram of the cell impedance sensor based on DFP-ITO electrode prepared in this embodiment is as follows image 3 shown.

[0058] For the convenience of cell culture and electrochemical impedance spectroscopy measurement, the photosensitive dry film-indium tin oxide electrode and the measuring cell 5 prepared in Example 2 were fixed by polymethyl methacrylate (PMMA) clamps, so that the DFP-ITO electrode was placed At the bottom of the measuring cell, the volume of the measuring cell is 500 μL. DFP-ITO electrode, measuring cell assembly diagram and physical picture Figure 4 shown.

[0059] The cell impedance sensor based on the photosensitive dry film-indium tin oxide electrode includes the photosensitive dry film-indium tin oxide electrode prepared in Example 2 and the measuring cell 5, and the measuring cell 5 is placed in the photosensitive dry film-indium tin oxide electrode. On the...

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Abstract

The invention belongs to the technical field of cell impedance sensors and particularly relates to a cell impedance sensor based on a DFP-ITO electrode and application of the cell impedance sensor. The DFP-ITO electrode comprises a conductive substrate layer and an insulating layer located on the conductive substrate layer, wherein the insulating layer is provided with electrode holes, the conductive substrate layer is further provided with lead ports, the conductive substrate layer is indium tin oxide conductive glass, and the insulating layer is a light-sensing dry film. The cell impedance sensor comprises the DFP-ITO electron and a measuring cuvette. The cell impedance sensor is simple in structure, easy to machine, capable of acquiring the morphology and impedance feature values of cells on the DFP-ITO electrode at the same time, capable of being popularized in common labs, and applicable to research fields such as cell physiology and pathology behaviors and drug screening.

Description

technical field [0001] The invention belongs to the technical field of cell impedance sensors, and in particular relates to a DFP-ITO electrode-based cell impedance sensor and its application. The application specifically involves the simultaneous detection of cell morphology and impedance information by using the cell impedance sensor. Background technique [0002] As a continuous and non-invasive in vitro cell analysis technology, cell impedance sensor can detect biological behaviors such as cell adhesion, proliferation, apoptosis, etc. have been extensively researched and applied. [0003] The cell impedance sensor technology was first reported by Giaever and Keese et al. The cell impedance biosensor constructed by this technology is constructed by a pair of gold two-electrode system, including a working electrode with a diameter of 250 μm and a large-area counter electrode. When cells undergo morphological changes on the electrode surface, the existence of cells as freq...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/26G01N21/84
Inventor 李远刘北忠龚放
Owner AFFILIATED YONGCHUAN HOSPITAL OF CHONGQING MEDICAL UNIV
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