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Water-soluble vitamin D3 nanometer particles and preparation method thereof

A technology of water-soluble vitamins and nanoparticles, which is used in food preparation, food forming, and food ingredients as solubility improvers. and other problems, to achieve the effects of good colloidal stability and chemical stability, good colloidal stability and good water solubility

Active Publication Date: 2015-11-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] This method overcomes existing preparation vitamin D 3 The preparation process of nanoparticle method is complicated, the obtained particle size is large, and the carrier raw material is expensive. By utilizing the interaction between macromolecules and small molecules, the micro-nano colloidal structure is formed by manipulating simple process conditions, and the prepared vitamin D 3 Nanoparticles have small particle size, good monodispersity, good water resolubility, and good colloidal and chemical stability

Method used

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  • Water-soluble vitamin D3 nanometer particles and preparation method thereof
  • Water-soluble vitamin D3 nanometer particles and preparation method thereof
  • Water-soluble vitamin D3 nanometer particles and preparation method thereof

Examples

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Embodiment 1

[0042] A water-soluble vitamin D 3 A method for preparing nanoparticles, comprising the steps of:

[0043] Step 1: Uniformly disperse corn protein concentrate (protein content: 87.5±0.6%, dry basis) in deionized water to prepare a 3% (w / v, g / mL) dispersion. Heat the dispersion in a water bath to 50° C. and adjust the pH to 9.0 with 1M NaOH, add Alcalase protease at a mass ratio of 2% for enzymolysis, and keep the temperature and pH constant for 2 hours. After the enzymatic hydrolysis, the pH of the material was adjusted to 7.0 with 1M HCl, and then heated in a boiling water bath for 5 minutes to inactivate the enzyme. After the hydrolyzate was centrifuged at 10000r / min for 20min, the supernatant was passed through a 0.65μm microfiltration membrane and put into a dialysis bag with a molecular weight cutoff of 100Da, and dialyzed in deionized water at 4°C for 24h, every 8 hours Change the dialysate once. After the dialysis, the zein hydrolyzate was obtained through rotary eva...

Embodiment 2

[0056] A preparation of vitamin D based on corn protein hydrolyzate 3 The method for nanoparticles, comprising the steps of:

[0057] Step 1: Uniformly disperse corn protein concentrate (protein content: 87.5±0.6%, dry basis) in deionized water to prepare a 3% (w / v, g / mL) dispersion. The dispersion solution was heated in a water bath to 50° C. and then adjusted to pH 9.0 with 1M NaOH. Alcalase protease was added at a mass ratio of 3% for enzymatic hydrolysis. The enzymatic hydrolysis was carried out for 1 h while maintaining a constant temperature and pH. After the enzymatic hydrolysis, the pH of the material was adjusted to 7.0 with 1M HCl, and then heated in a boiling water bath for 5 minutes to inactivate the enzyme. After the hydrolyzate was centrifuged at 10000r / min for 20min, the supernatant was passed through a 0.65μm microfiltration membrane and put into a dialysis bag with a molecular weight cutoff of 200Da, and dialyzed in deionized water at 4°C for 24h, every 8 hou...

Embodiment 3

[0064] A preparation of vitamin D based on corn protein hydrolyzate 3 The method for nanoparticles, comprising the steps of:

[0065] Step 1: Uniformly disperse corn gluten powder (protein content: 53.2±0.8%, dry basis) in deionized water to prepare a 5% (w / v, g / mL) dispersion. The dispersion solution was heated to 50°C in a water bath and then adjusted to pH 8.0 with 1M NaOH. Alcalase protease was added at a mass ratio of 1% for enzymatic hydrolysis. Under the condition of maintaining constant temperature and pH, the enzymatic hydrolysis was carried out for 3 hours. After the enzymatic hydrolysis, the pH of the material was adjusted to 6.0 with 1M HCl, and then heated in a boiling water bath for 5 minutes to inactivate the enzyme. After the hydrolyzate was centrifuged at 10000r / min for 20min, the supernatant was passed through a 0.65μm microfiltration membrane and put into a dialysis bag with a molecular weight cutoff of 100Da, and dialyzed in deionized water at 4°C for 24h,...

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Abstract

The invention discloses water-soluble vitamin D3 nanometer particles and a preparation method thereof. The preparation method comprises the following steps: firstly, enabling corn protein to be uniformly dispersed in deionized water to obtain a dispersed solution, heating the dispersed solution, adjusting the PH value, adding Alcalase protease for enzymolysis, performing enzyme deactivation, and performing dialysis so as to obtain corn protein hydrolyzate; then, selecting a vitamin D3 mother liquid prepared with an organic solvent; enabling the corn protein hydrolyzate to be dissolved in a PBS solution; dropwise adding the vitamin D3 mother liquid in the corn protein hydrolyzate solution, and performing centrifugation so as to obtain a corn protein hydrolyzate-vitamin D3 nanometer particle solution; and freeze-drying the corn protein hydrolyzate-vitamin D3 nanometer particle solution so as to obtain water-soluble vitamin D3 nanometer particles. According to the water-soluble vitamin D3 nanometer particles and the preparation method thereof disclosed by the invention, the secondary interaction of the corn protein hydrolyzate and the vitamin D3 is utilized for preparing the water-soluble vitamin D3 nanometer particles. The water-soluble vitamin D3 nanometer particle products are good in solubility, small in particle diameter, high in load rate and good in chemical stability and colloid stability, greatly improve the processing stability of the vitamin D3, and can be applied as food ingredients.

Description

technical field [0001] The present invention relates to vitamin D 3 , specifically involving a water-soluble form of vitamin D 3 Nanoparticles and methods for their preparation; water-soluble vitamin D 3 Nanoparticles can be used in health food and functional food. Background technique [0002] In the past ten years, the research on peptide self-assembly and amphiphilicity has been a hot topic, and there are also many studies using the self-assembly properties of peptides to explore its use in active ingredient embedding and drug delivery. Compared with proteins, peptides have the characteristics of good solubility, low antigenicity, and easier absorption, and peptides obtained from food are considered safe and healthy ingredients, with the advantages of small molecular weight, low cost, and high activity. [0003] The research on corn hydrolyzate is mainly to study its physiological activities such as anti-oxidation, lowering blood pressure, anti-tumor, and promoting alc...

Claims

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Application Information

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IPC IPC(8): A23L1/303A23P1/04A23L33/155
CPCA23V2002/00A23V2200/238A23V2250/7106A23V2300/20A23V2300/10
Inventor 杨晓泉林媛王永辉
Owner SOUTH CHINA UNIV OF TECH
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