Application of Vermiculite and Perlite as Substrate for the Symbiotic Culture of Rhizobia and Robinia pseudoacacia

Abstract

The invention discloses an application of using vermiculite and perilite as a nodule bacteria and locust symbiotic culture medium. By the adoption of a medium bag cultivation technology, the medium of vermiculite and perlite mixed according to the proportion of 2:1 is loaded into a transparent plastic bag, a non-nitrogen nutrient solution is irrigated for sterilization, then a locust seedling is planted inside the bag, nodule bacteria are inoculated, and then greenhouse culture is performed. Compared with a traditional water culture method, by the utilization of the application method, the nodule bacteria and the locust can form root nodule tissue at the eighth day, and the number of root nodules and the plant aboveground part biomass are obviously higher than those of water culture. The method overcomes the defects that according to the traditional water culture method, gas permeability is poor, the pollution rate is high, symbiotic system establishment time is long, the number of formed root nodules is small, and the leguminous plant biomass is small, and can be applied to rapid establishment of a leguminous plant and root nodule bacteria symbiotic nitrogen fixation system, plant and microorganism interaction research, endangered leguminous plant protection and cultivation of robust seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to the application of vermiculite and perlite as substrates for symbiotic culture of rhizobia and black locust. Background technique [0002] Rhizobium is an important class of agricultural microorganisms. They can form root nodules in symbiosis with legumes, reduce atmospheric nitrogen to ammonia, and provide the host plants with the nitrogen nutrients they need. This symbiotic system is known to have the strongest biological nitrogen fixation capacity. Its annual nitrogen fixation accounts for more than 50% of the world's total biological nitrogen fixation, which is equivalent to twice the world's industrial synthetic nitrogen fertilizer, and is the main link of the global nitrogen cycle. As a kind of important green manure or ecological protection plants, leguminous plants, such as chrysanthemum, sweet rhinoceros, amorpha, black locust, etc., play an importa...

AI Technical Summary

Problems solved by technology

However, there are many defects in the cultivation process, mainly showing that the concentration, temperature, and pH value of the nutrient solution are prone to drastic changes, poor air permeability, low dissolved oxygen, and poor environmental stability of the root system.

In addition, for seeds with large volume and weight, such as soybeans, broad beans, etc., the filter paper sometimes has certain difficulties in supporting the seeds, and is easily polluted by external microorganisms, which affects the formation of the symbiotic system.

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