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Applications of liquid crystal sensor in detection of organophosphorus pesticides and carbamate pesticides

A technology of carbamates and organophosphorus pesticides, applied in the preparation of test samples, instruments, measuring devices, etc., can solve the problems of detection instruments that are not suitable for fast, sensitive, trace pesticide detection, complex experimental methods for sample processing, etc. , to achieve the effect of short sample processing time, low detection limit and low reagent consumption

Inactive Publication Date: 2016-01-13
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods require long-term sample processing, complex experimental methods, and professional detection instruments, and are not suitable for rapid, sensitive, and trace pesticide detection.

Method used

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  • Applications of liquid crystal sensor in detection of organophosphorus pesticides and carbamate pesticides
  • Applications of liquid crystal sensor in detection of organophosphorus pesticides and carbamate pesticides
  • Applications of liquid crystal sensor in detection of organophosphorus pesticides and carbamate pesticides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The liquid crystal sensor is prepared by the following method:

[0045] a. Soak the glass slide in 60mL glass slide washing solution, soak for 30min at 75°C, then wash with water, absolute ethanol, and methanol for 3 times respectively, blow dry with nitrogen, and dry overnight at 110°C. Placed in octadecyltrichlorosilane-heptane solution with a concentration of 5mM for 30min, rinsed with dichloromethane, and dried with nitrogen to obtain the treated substrate;

[0046] b. Add a liquid crystal-heptane solution with a volume fraction of 1% dropwise to the upper surface of the treated substrate obtained in step a, and prepare a liquid crystal sensor after the heptane volatilizes under the condition of oxygen barrier.

[0047] The application of the liquid crystal sensor in the detection of organophosphorus pesticides and carbamate pesticides, the steps are as follows:

[0048] (1) Dissolve Zhongbucarb in phosphate buffer solution for 30 to 40 minutes by ultrasonic waves,...

Embodiment 2

[0055] The preparation method of the liquid crystal sensor is as described in Example 1.

[0056] The application of the liquid crystal sensor in the detection of organophosphorus pesticides and carbamate pesticides, the steps are as follows:

[0057] (1) Dimethoate is dissolved in phosphate buffer solution by ultrasonic wave for 30-40 min, and then prepared into dimethoate concentration gradient solutions with a concentration of 0.00001-1 μg / mL;

[0058] The composition of the phosphate buffer solution is as follows: 10 mM phosphoric acid, 138 mM NaCl, 2.7 mM KCl; pH=7.4;

[0059] (2) Add acetylcholinesterase to the solution to be tested prepared in step (1), the amount of acetylcholinesterase added is 0.01 mg / mL, react at 37° C. for 15 minutes, and obtain an enzyme inhibition solution;

[0060] (3) adding tetradecylcholine chloride to the enzyme inhibitory solution prepared in step (2), so that the concentration of tetradecylcholine chloride is 0.1 mM, and reacting at 37° C...

Embodiment 3

[0063] The preparation method of the liquid crystal sensor is as described in Example 1.

[0064] The application of the liquid crystal sensor in the detection of organophosphorus pesticides and carbamate pesticides, the steps are as follows:

[0065] (1) Dimethoate was dissolved in lake water for 30 to 40 minutes by ultrasonic waves, and prepared into dimethoate concentration gradient solutions with a concentration of 0.00001-1 μg / mL;

[0066] (2) Add acetylcholinesterase to the solution to be tested prepared in step (1), the amount of acetylcholinesterase added is 0.01 mg / mL, react at 37° C. for 15 minutes, and obtain an enzyme inhibition solution;

[0067] (3) adding tetradecylcholine chloride to the enzyme inhibitory solution prepared in step (2), so that the concentration of tetradecylcholine chloride is 0.1 mM, and reacting at 37° C. for 15 min to obtain an enzyme reaction solution;

[0068] (4) 1 μL of the enzyme reaction solution prepared in step (3) was dropped on th...

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Abstract

The invention relates to applications of a liquid crystal sensor in detection of organophosphorus pesticides and carbamate pesticides. An application method comprises following steps: (1) a sample to be detected is dissolved in a phosphate buffer solution so as to obtain a solution to be detected; (2) acetylcholin esterase is added into the solution, and an enzyme inhibited solution is obtained via reaction; (3) myristoylcholine chloride is added into the enzyme inhibited solution, and an enzyme reaction solution is obtained via reaction; and (4) the enzyme reaction solution is added onto the liquid crystal sensor dropwise, and a polarizing microscope is used for observation. The application method using the liquid crystal sensor to detect organophosphorus pesticides and carbamate pesticides in liquid phase is low in detection limit; detection instrument is easily available; a detection method is simple, rapid, and sensitive; reagent consumption is less; sample processing time is short; and problems of the convention detection method that detection is complex, and cost is high are solved.

Description

technical field [0001] The invention relates to the application of a liquid crystal sensor in detecting organophosphorus pesticides and carbamate pesticides, and belongs to the technical field of enzyme inhibition method detection. Background technique [0002] Organophosphorus and carbamate compounds can inhibit the activity of acetylcholinesterase, increase the content of neurotransmitter acetylcholine in organisms, and lead to disease and even death of organisms. Based on this mechanism of action, organophosphorus pesticides and carbamate pesticides are widely used as insecticides in agricultural production. In recent years, due to the large number of irregular use of pesticides, their residues in water bodies have increasingly become a major hidden danger to public safety, and have received widespread attention. [0003] Reported methods for the detection of organophosphorus and carbamate pesticides, such as mass spectrometry, gas chromatography, high performance liquid...

Claims

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Application Information

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IPC IPC(8): G01N21/84G01N1/28
Inventor 于丽王毅赵英渊田彤彤郭永先
Owner SHANDONG UNIV
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