Fluorescence nanometer probe for imaging and preparation method thereof

A fluorescent nanoprobe and imaging technology, applied in the field of nanoprobes, can solve the problems of toxicity, large polymer particle size, and inability to instant imaging, and achieve the effects of high accuracy, simple preparation method, and cheap preparation method.

Inactive Publication Date: 2016-02-10
SHANGHAI FIRST MATERNITY & INFANT HOSPITAL
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Problems solved by technology

Nanoscale polymers include conjugated polymers, nanogel polymers, nanocomposites, liposome nanopolymers, etc., and different skeleton materials can be selected (non-toxic substances such as pullulan, γ-glutamic acid, and phospholipids) , by connecting different imaging agents inside or on its surface to meet the requirements required as a sentinel lymph node tracer, and it can also be connected with multiple imaging agents for multimodal imaging, but the existing polymer particles The diameters are large, larger than 50nm, and cannot be used for instant imaging of SLN
Although they have good biocompatibility and are low-toxic substances, they are likely to become toxic substances when mixed with imaging agents

Method used

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  • Fluorescence nanometer probe for imaging and preparation method thereof
  • Fluorescence nanometer probe for imaging and preparation method thereof
  • Fluorescence nanometer probe for imaging and preparation method thereof

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Embodiment 1

[0037] 50 mg of dextran and 30 mg of p-nitrophenyl carbonate (PNC) were reacted at 25°C for 40 hours to obtain Dextran-PNC, and 20 mg of Dextran-PNC and 50 mg of mercaptoethylamine were reacted at 25°C for 30 hours to obtain mercaptolation Dextran (Dextran-SH), Dextran-SH (50mg,) and 2-pyridine cysteine ​​hydrochloride (75mg) were dissolved in 5ml of water, reacted overnight at room temperature, purified by ultrafiltration (MWCO3000), and lyophilized to obtain Dex- SS-NH2; activate the carboxyl group of deoxycholic acid (DCA100mg) by EDC method; dissolve Dex-SS-NH2 (10mg) with 3mL of water, neutralize hydrochloric acid with pyridine and add it to the above-mentioned activated DCA solution , stirred at room temperature for 48h. The above reaction solution was precipitated with 30 mL of ethanol, centrifuged at 7000×g for 5 min, dissolved in water, dialyzed (MWCO3500) and freeze-dried to obtain the final product Dex10k-SSDCA. Take Dex10k-SSDCA (15 mg) and water-soluble cyanine d...

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Abstract

The invention provides a fluorescence nanometer probe for imaging and a preparation method thereof. The fluorescence nanometer probe for imaging possesses the structural formula shown as a formula (I), and the substance carries fluorophore. The preparation method for the probe comprises: reacting glucan with PNC to obtain Dextran-PNC; reacting Dextran-PNC with aminoethyl mercaptan to obtain Dextran-SH; reacting Dextran-SH with PDA to obtain Dex-SS-NH2; reacting activated deoxycholic acid with Dex-SS-NH2 under a room temperature condition to obtain Dex-SS-DCA; and dissolving Dex-SS-DCA and a fluorescence dye Cy7 in dimethyl sulfoxide, performing a reaction in the dark at room temperature, and then performing dialysis and freeze drying, so as to obtain Dextran-Cy7 powder. The probe possesses good biological compatibility, long detention time in SLN, high efficiency, high resolution, high light drifting resistance, no toxicity and innocuousness and other characteristics.

Description

technical field [0001] The invention relates to the technical field of nanoprobes, in particular to a fluorescent nanoprobe for imaging and a preparation method thereof. Background technique [0002] In recent years, near-infrared fluorescence imaging has become a new technology and new trend of foreign sentinel lymph node biopsy, and has the prospect of replacing radionuclides. Near-infrared fluorescence imaging is based on the use of biological tissue in the near-infrared (emission wavelength 650-1000nm) region of autologous light absorption or low fluorescence intensity, low light scattering in dense media (such as tissue), strong penetration of excitation light, and autofluorescence. It is a new imaging technology that can effectively image the deeper tissues of the organism due to the characteristics of small background interference. The near-infrared fluorescent dye indocyanine green has been approved by the US Food and Drug Administration for clinical trials in lymph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00C09K11/06C08B37/02
Inventor 庄志刚李洁静
Owner SHANGHAI FIRST MATERNITY & INFANT HOSPITAL
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