Preparation method and application of anti-bacterial and anti-inflammation sow thistle ointment

An antibacterial, anti-inflammatory, and bitter vegetable technology, which can be used in antibacterial drugs, pharmaceutical formulations, and medical preparations containing active ingredients, etc. Increased antibacterial and anti-inflammatory effect, improved antibacterial and anti-inflammatory ability, and high cure rate

Inactive Publication Date: 2016-02-24
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are some patents on beverages, health foods or ointments that add bitter herbs to the raw materials for preparation. However, in these existing technologies, the amount of bitter herbs added is very small, and it is difficult to extract bitter herbs due to lack of proper extraction and p

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0044] An antibacterial and anti-inflammatory bitter vegetable ointment is prepared by using a stepwise extraction method to extract raw materials, wherein the raw material is mainly bitter vegetable, including the following steps: Step 1. Quick-freeze bitter vegetable at minus 23-25°C 30-90 minutes, then vacuum, 110-60 ℃ gradient cooling freeze-drying, crushing, the obtained raw material powder is mixed and extracted with 30-70 times the mass of 65-80% ethanol solution, after the extraction, the ethanol is evaporated, and the remaining first Extract, dissolve the first extract in 4-15 times the volume of water to obtain the first extract aqueous solution; step 2, mix and extract the first extract aqueous solution with 0.3-2 times the volume of petroleum ether, separate the water phase to obtain the first extract Second extract; Step 3. Mix and extract the second extract obtained with 0.3-2 times the volume of ethyl acetate, separate the aqueous phase to obtain the third extract...

Example Embodiment

[0056] Example 1

[0057] 1. Weigh 50g of bitter vegetable powder and mix with 2000mL of 70% ethanol solution, and boil slightly on the electric stove for 3 minutes to fully rupture the cells of bitter vegetable, so that the effective ingredients in the bitter vegetable are fully dissolved.

[0058] 2. After the beaker is placed at room temperature, centrifuge it in a centrifuge at a speed of 3000 r / min for 10 minutes. After centrifugation, the supernatant is separated, and then ultrasonically assisted for extraction for 30 minutes. Rotate the bitter vegetable supernatant with a rotary evaporator at 70°C to evaporate all the alcohol, leaving about 40 mL of thick paste-like substance to obtain the first extract;

[0059] 3. Dilute the first extract to 200mL with distilled water to obtain the first extract aqueous solution. Add petroleum ether 3 times to mix and extract. The amount of petroleum ether added for 3 times is 60mL, 60mL, 80mL, and the final separated The water phase is the...

Example Embodiment

[0063] Example 2

[0064] 1. Weigh 20 g of bitter vegetable powder in a 1000 mL beaker and soak in 600 mL of 75% ethanol solution. Seal the mouth of the beaker with a plastic wrap, and boil for 2 minutes on an electric stove, so that the cells of the bitter vegetable are fully ruptured, so that the effective ingredients in the bitter vegetable are fully dissolved.

[0065] 2. After the beaker is placed at room temperature, centrifuge it in a centrifuge at a speed of 4000 r / min for 8 minutes. After centrifugation, the supernatant is separated, and then ultrasonically assisted for extraction for 25 minutes. Rotate the bitter vegetable supernatant with a rotary evaporator at 55°C to evaporate all the alcohol, leaving about 10 mL of thick paste-like substance to obtain the first extract;

[0066] 3. The first extract is added to distilled water to make the volume to 100mL, and then the first extract aqueous solution is obtained. Petroleum ether is added in 3 times to mix and extract. Th...

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Abstract

The invention discloses a preparation method and application of anti-bacterial and anti-inflammation sow thistle ointment. The anti-bacterial and anti-inflammation sow thistle ointment is obtained by extracting raw materials according to a sequential extraction method, wherein sow thistle serves as a main raw material. The preparation method comprises the following steps: drying the raw materials; smashing the dried raw materials; mixing raw material powder with an ethanol solution to obtain a mixture I; heating the mixture I to boil; carrying out ultrasonic shaking and centrifugal separation to obtain a supernatant fluid; evaporating ethanol in the supernatant fluid, wherein the residue serves as a first extract; dissolving the first extract in water being 4 to 15 times of the first extract in volume to obtain a first extract water solution; conducting extraction with ligarine on the first extract water solution, carrying out extraction with vinegar naphtha and n-butanol sequentially, and evaporating n-butanol after extraction is finished to obtain a thick paste; mixing the thick paste with water being 1 to 15 times of the thick paste in volume to obtain a uniform raw material extracting solution, and mixing the raw material extracting solution with an ointment base to obtain the uniform sow thistle ointment. The sow thistle ointment can be applied to treatment of haemorrhoids, skin damage, acne or mammitis by being smeared on an affected part directly.

Description

technical field [0001] The invention relates to the field of ointment, in particular to a preparation method and application of bitter vegetable ointment with antibacterial and anti-inflammatory effects. Background technique [0002] Bitter cabbage contains a variety of nutrients needed by the human body. Modern medicine has proved that long-term consumption of bitter cabbage and its products can prevent and treat many diseases. The polysaccharides contained in the leaves of bitter herbs have two-way effects of anti-diarrhea and anti-constipation; the plant proteins contained in bitter herbs have good anti-tumor, antibacterial, anti-aging effects; bitter herbs also contain dandelion sterols, choline and other ingredients , has a strong bactericidal effect on hemolytic streptococcus and Staphylococcus aureus drug-resistant strains, and also has a certain killing effect on Shigella, pneumococcus, Pseudomonas aeruginosa, meningococcus, diphtheria bacillus, etc. Hepatitis, cold...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61K36/8968A61K9/06A61P9/14A61P17/10A61P17/02A61P15/14A61P31/04A61K31/60
CPCA61K36/28A61K9/0014A61K9/0031A61K9/06A61K31/60A61K36/11A61K36/185A61K36/25A61K36/34A61K36/36A61K36/428A61K36/484A61K36/489A61K36/534A61K36/634A61K36/71A61K36/748A61K36/808A61K36/81A61K36/88A61K36/8888A61K36/8968A61K47/06A61K2236/17A61K2236/333A61K2236/35A61K2236/39A61K2236/51A61K2300/00
Inventor 霍莉李军姜春燕张秀敏李晓幸
Owner HEBEI UNIVERSITY
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