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Application of Ganoderma lucidum triterpene acid active part in the preparation of blood lipid-lowering health products and medicines

A technology of Ganoderma lucidum triterpene acid and active site, applied in the field of medicine, can solve problems such as unreported research on Ganoderma lucidum triterpene acid, and achieve the effects of being conducive to market promotion and utilization, improving yield, and high yield

Active Publication Date: 2018-07-31
杭州医学院资产经营有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although Ganoderma lucidum has a long history of being used as a precious fungi and algae medicinal material in my country, so far there is no report on the research on the triterpenic acid of Ganoderma lucidum in experimental animal models of hyperlipidemia

Method used

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  • Application of Ganoderma lucidum triterpene acid active part in the preparation of blood lipid-lowering health products and medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 (preparation of active site of Ganoderma lucidum triterpene acid)

[0042] (1) Take Ganoderma lucidum fruiting body medicinal materials (Zhejiang Wuyangtang Pharmaceutical Co., Ltd.), dry at 65°C, pulverize, and pass through a 100-mesh sieve to obtain dry powder; the dry powder is added with distilled water according to the ratio of material to liquid 1g: 20mL, and the temperature is 50°C, pH5. Under 5 conditions, 3.0% of the compound enzyme was added relative to the mass of the dry powder. The compound enzyme is composed of 50% neutral protease, 25% cellulase and 25% pectinase by weight, the enzymolysis time is 6h, the enzyme is killed, cooled, and suction filtered to obtain an enzymolysis solution, which is used for later use;

[0043] (2) Take the enzymatic hydrolysis solution prepared in the above step (1), add petroleum ether to extract and remove fat, then continue adding ethyl acetate to the enzymatic hydrolysis solution after degreasing to obtain th...

Embodiment 2

[0047] Embodiment 2 (preparation of active site of Ganoderma lucidum triterpene acid)

[0048] (1) Take Ganoderma lucidum fruiting body medicinal materials (Zhejiang Wuyangtang Pharmaceutical Co., Ltd.), dry at 65°C, pulverize, and pass through a 100-mesh sieve to obtain dry powder; the dry powder is added with distilled water according to the material-to-liquid ratio of 1g:15mL, and the temperature is 40°C, pH4. Under the condition of 0, 2.0% of the compound enzyme is added relative to the mass of the dry powder. The compound enzyme is composed of 30% neutral protease, 35% cellulase and 35% pectinase by weight, and the enzymatic hydrolysis time is 5h. Enzyme, cooling, suction filtration to obtain an enzymatic hydrolysate, for use;

[0049] (2) Take the enzymatic hydrolysis solution prepared in the above step (1), add petroleum ether to extract and remove fat, then continue adding ethyl acetate to the enzymatic hydrolysis solution after degreasing to obtain the ethyl acetate e...

Embodiment 3

[0053] Embodiment 3 (preparation of Ganoderma lucidum triterpene acid active site)

[0054] (1) Take Ganoderma lucidum fruiting body medicinal material (Zhejiang Wuyangtang Pharmaceutical Co., Ltd.), dry at 65°C, pulverize, and pass through a 100-mesh sieve to obtain dry powder; the dry powder is added with distilled water according to the material-to-liquid ratio of 1g: 25mL, and the temperature is 60°C and pH 6. Under the condition of 0, 4.0% of the compound enzyme is added relative to the mass of the dry powder. The compound enzyme is composed of 80% neutral protease, 10% cellulase and 10% pectinase by weight. The enzymatic hydrolysis time is 7h. Enzyme, cooling, suction filtration to obtain an enzymatic hydrolysate, for use;

[0055] (2) Take the enzymatic hydrolysis solution prepared in the above step (1), add petroleum ether to extract and remove fat, then continue adding ethyl acetate to the enzymatic hydrolysis solution after degreasing to obtain the ethyl acetate extr...

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Abstract

The invention discloses application of an active part of ganoderma triterpene acid in preparing hypolipidemic health care products and drugs. A preparation method of the active part comprises the following steps: crushing a lucid ganoderma fruiting body medicinal material so as to obtain dry powder, adding complex enzyme to the dry powder in water so as to enzymatically hydrolyze the dry powder, inactivating enzyme, cooling and suction-filtering so as to obtain enzymatic hydrolysate; extracting and degreasing the enzymatic hydrolysate by virtue of petroleum ether, and then extracting with the addition of ethyl acetate so as to obtain a lucid ganoderma ethyl acetate extract; alkalifying the lucid ganoderma ethyl acetate extract firstly, then rinsing the alkalified lucid ganoderma ethyl acetate extract to be neutral so as to obtain an alkalified water layer, and then acidifying the alkalified water layer and extracting through ethyl acetate so as to obtain coarse ganoderic acid; and separating the coarse ganoderic acid by virtue of a silica gel column, eluting the ganoderic acid by virtue of a chloroform and methanol system in a gradient mode, and conducting thin-layer chromatography identification, filtration and drying by distillation so as to obtain the active part of ganoderma triterpene acid. The active part of ganoderma triterpene acid, which can be obtained through such steps as enzymatic hydrolysis with the complex enzyme, extracting, separating and the like, has a significant hypolipidemic effect; therefore, the active part can be used for preparing hypolipidemic health care products and drugs.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of an active part of Ganoderma lucidum triterpene acid in the preparation of blood lipid-lowering health care products and medicines. Background technique [0002] With the improvement of people's living standard and the change of diet structure, the incidence of hyperlipidemia is increasing year by year, and it shows a trend of younger people. Research data show that hyperlipidemia is the pathological basis of atherosclerosis, which leads to a series of cardiovascular and cerebrovascular diseases that endanger human health, such as hypertension, stroke, coronary heart disease and myocardial infarction. It is one of the risk factors for diabetes and other important organ diseases. Modern medicine believes that dyslipidemia refers to the abnormal amount and quality of lipids in blood, mainly manifested as plasma or serum total cholesterol (TC), total triglyceride (TG), low...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/575A61K36/074A61P3/06A23L33/00
CPCA23V2002/00A61K9/1652A61K9/2009A61K9/2059A61K9/4866A61K31/575A61K36/074A61K2236/19A61K2236/33A61K2236/55A23V2200/30A23V2200/3262A23V2250/208
Inventor 刘月环么春艳王志远吴学谦
Owner 杭州医学院资产经营有限公司