O. verruca bzip1 gene
A wart grain wild rice, gene technology, applied in the field of plant biology, can solve the problems of resistant varieties becoming susceptible, frequent variation of physiological races, etc., to achieve the effect of enhancing disease resistance
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[0015] 1. Cloning of the OmbZIP1 gene of Oryza sativa
[0016] 1.1. RNA preparation of Oryza sativa
[0017] (1) Reagents:
[0018] Solution I ligase was purchased from TaKaRa Company; plasmid extraction kit was purchased from Beijing Kangwei Century Biotechnology Co., Ltd.; DNA fragment recovery kit was purchased from Promega Company; restriction endonuclease and cDNA first-strand synthesis kit were purchased from NEB company; TRIzol reagent was purchased from Invitrogen; high-fidelity DNA polymerase was purchased from TOYOBO; IPTG (Isopropyl-1thio-β-galactoside, isopropyl-thio-β-D-galactoside); X-gal (5 -bromo-4-chloro-3-indolyl-β-D-galactoside, 5-bromo-4-chloro-3-indole-β-thiogalactoside); Kanamycin (Kanamycin, Kan); Ampicillin Penicillin (Ampicillin, Amp); Streptomycin (Streptomycin, Str); Liquid nitrogen; Diethylprocarbonate (DEPC); Absolute ethanol; Chloroform; Isopropanol; Na2EDTA; Glacial acetic acid; Glycerin; Tryptone; Yeast extract; NaCl; agar were synthesized by...
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