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Liver cell preserving fluid and preparation method and application

A liver cell and preservation solution technology, applied in the field of liver cell preservation solution and its preparation, can solve the problems of easy swelling and death, low vitality and other problems, and achieve the effect of strong buffering force of solution penetration system, reducing side effects and stable components

Active Publication Date: 2016-05-04
WUHAN TOGO MEDITECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the defects of low vigor and easy to swell and die in L-02 cell culture in the prior art, and to provide a liver cell preservation solution with stable components, multiple nutrients, and strong buffering force of the solution osmosis system and its preparation Methods and Applications

Method used

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  • Liver cell preserving fluid and preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Liver cell preservation solution 1, containing 10ml / L hydroxyethyl starch 130 / 0.4 sodium chloride injection, 2g / L glucose, 10g / L sodium glutamate, 200mg / L vitamin C, 5g / L phenol red , 10mg / L glucuronolactone, 50mg / L polyene phosphatidylcholine, cell buffer and amino acid mixture;

[0026] The volume ratio of the cell buffer solution to the amino acid mixture solution is 1:1, and the content of the mixture solution in the liver cell preservation solution is 800ml / L;

[0027]The content of each component in the cell buffer is: 10mM KCl, 2mM CaCl 2 , 290mM NaCl, H 2 CO 3 / NaHCO 3 , NaH 2 PO 4 / Na 2 HPO 4 1. Sterile water, the pH of the cell buffer is 7.35;

[0028] The content of each component in the amino acid mixture is: 3.5g / L lysine, 1.5g / L tryptophan, 4.3g / L phenylalanine, 4.6g / L methionine, 1.0g / L threonine, 5.8 g / L isoleucine, 3.6g / L leucine, 5.4g / L valine, 0.2g / L sodium bisulfite, 9g / L sorbitol.

Embodiment 2

[0030] Liver cell preservation solution 2, containing 12ml / L hydroxyethyl starch 130 / 0.4 sodium chloride injection, 1g / L glucose, 12g / L sodium glutamate, 220mg / L vitamin C, 6g / L phenol red , 12mg / L glucuronolactone, 60mg / L polyene phosphatidylcholine, cell buffer, amino acid mixture;

[0031] The volume ratio of the cell buffer solution to the amino acid mixture solution is 1:2, and the content of the mixture solution in the liver cell preservation solution is 850ml / L;

[0032] The content of each component in the cell buffer is: 7mM KCl, 2.6mM CaCl 2 , 276mM NaCl, H 2 CO 3 / NaHCO 3 , NaH 2 PO 4 / Na 2 HPO 4 1. Sterile water, the pH of the cell buffer is 7.4, and the osmotic pressure content is 304mOsm / L;

[0033] The content of each component in the amino acid mixture is: 6.5g / L lysine, 0.5g / L tryptophan, 7.4g / L phenylalanine, 1.8g / L methionine, 3.8g / L threonine, 2.5 g / L isoleucine, 6.3g / L leucine, 2.6g / L valine, 1.3g / L sodium bisulfite, 9g / L sorbitol.

Embodiment 3

[0035] Liver cell preservation solution 3, containing 14ml / L hydroxyethyl starch 130 / 0.4 sodium chloride injection, 1.6g / L glucose, 14g / L sodium glutamate, 240mg / L vitamin C, 7g / L phenol Red, 14mg / L glucuronolactone, 70mg / L polyene phosphatidylcholine, cell buffer, amino acid mixture;

[0036] The volume ratio of the cell buffer solution to the amino acid mixture solution is 1:1.3, and the content of the mixture solution in the liver cell preservation solution is 840ml / L;

[0037] The content of each component in the cell buffer is: 9.2mM KCl, 2.3mM CaCl 2 , 285mM NaCl, H 2 CO 3 / NaHCO 3 , NaH 2 PO 4 / Na 2 HPO 4 1. Sterile water, the pH of the cell buffer is 7.45, and the osmotic pressure is 308mOsm / L;

[0038] The content of each component in the amino acid mixture is: 4g / L lysine, 1.2g / L tryptophan, 5.3g / L phenylalanine, 3.2g / L methionine, 2.7g / L threonine, 3.5g / L isoleucine, 5.7g / L leucine, 5.0g / L valine, 0.4g / L sodium bisulfite, 10g / L sorbitol.

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Abstract

The invention discloses a liver cell preserving fluid and a preparation method and application thereof. The liver cell preserving fluid which is stable in ingredient, diverse in nutrient substance and strong in solution permeating system buffering power is formed by mixing hydroxyethyl starch 130 / 0.4 sodium chloride injection, glucose, sodium glutamate, vitamin C, phenol red, glucurolactone, polyene phosphatidyl choline, a cell buffering solution and an amino acid mixed solution. The liver cell preserving fluid can not only provide nutrition for liver cells, can also well maintain cell activity and accordingly plays a better effect in subsequent clinical use.

Description

technical field [0001] The invention relates to a cell preservation solution, in particular to a liver cell preservation solution and its preparation method and application, belonging to the field of biotechnology. Background technique [0002] In recent years, cell biotherapy technology has gradually replaced traditional drug therapy and played an important role in various diseases. Cell therapy refers to the use of cells with certain specific functions, which are treated by bioengineering methods through in vitro expansion and special culture, so that these cells can enhance immunity, kill pathogens and tumor cells, promote tissue and organ regeneration, and restore the body. therapeutic effect, so as to achieve the purpose of treating diseases. Cell therapy has gradually become the latest and best immunotherapy method for anti-tumor, anti-hepatitis B and C. [0003] Among them, L-02 liver cells are the seed cells in the treatment of liver disease cells. After in-depth ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 周平陈露闵娅兰郭伟李亚丽
Owner WUHAN TOGO MEDITECH CO LTD
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