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A strain of Pallidum bacterium degrading ofloxacin and its application

A technology of ofloxacin and Pallidum, which is applied in the field of environmental microorganisms, can solve problems such as damage to the balance of the ecosystem, allergic reactions, and difficult antibiotic removal, and achieve the effects of reducing wastewater treatment costs, protecting the ecological environment, and protecting human health

Active Publication Date: 2019-04-12
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, antibiotic pollutants have low vapor pressure and low hydrophobicity, and mainly exist in the water environment. The water contains a variety of refractory biotoxic substances and high concentrations of active antibiotics, which inhibit the growth of microorganisms in wastewater biochemical treatment. Therefore, The existing water treatment technology is difficult to completely remove antibiotics, some antibiotics will enter the water and soil along with the treated wastewater
The long-term existence of low-concentration antibiotics has a certain impact on the microbial community. A large number of studies have shown that the use of antibiotics can induce pathogenic bacteria to produce some pathogenic bacteria that can resist antibiotics. Usually, antibiotics in the range of micrograms per liter in aquatic systems will lead to drug-resistant strains. The increase of these drugs will destroy the balance of the ecosystem. Once these drug-resistant genes are transferred to pathogenic bacteria, the impact will have an effect on higher organisms through the food chain, which will increase the threat to human and animal health.
In addition, animal antibiotics will also enter the human food chain through meat products, causing the antibiotic content in various meat and dairy products to often exceed the standard, causing allergic reactions in humans, and even food poisoning in severe cases, and some antibiotics may have lethality. Teratogenic, carcinogenic, and mutagenic effects seriously interfere with human physiological functions and threaten human health

Method used

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  • A strain of Pallidum bacterium degrading ofloxacin and its application
  • A strain of Pallidum bacterium degrading ofloxacin and its application
  • A strain of Pallidum bacterium degrading ofloxacin and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Isolation and identification of ofloxacin-degrading strain OF-13.

[0027] Take 1.0ml of ofloxacin enriched bacteria solution, add 9.0ml sterile water, mix well to make 10 -1 The rich solution, and then draw 1.0ml prepared 10 -1 The enrichment solution was added to 9.0ml sterile water, mixed thoroughly to make 10 -2 The enrichment solution, and so on, carry out gradient dilution on the enrichment solution. Draw 0.1ml of the diluted solution of each gradient and spread it on the inorganic salt solid medium containing 500ug / L ofloxacin. The formula is: every liter contains 1.5g K 2 HPO 4 , 0.5g KH 2 PO 4 , 0.2g MgSO 4 ·7H 2 O, 1.0gNaCl, 1.0g (NH 4 ) 2 SO 4 , 0.05g anhydrous piperazine, 20g agar, pH 7.0, cultured at 30°C for 7 days. After 7 days, pick a single colony from the above inorganic salt solid medium, culture in 5ml LB liquid medium for 24 hours, centrifuge at 8000r / min for 2min, pour off the supernatant, add 5ml of sterile Shake well with w...

Embodiment 2

[0028] Example 2: Method for preparing strain OF-13 into immobilized pellets.

[0029] 1) Use an inoculation loop to pick a small amount of bacterial strains on the slant medium and inoculate them in LB liquid medium at 30°C and 160r / min for shaking culture to the logarithmic phase;

[0030]2) Centrifuge the above cultured bacterial solution at 10000r / min for 2min, discard the supernatant, add the same volume of sterile water, shake well and centrifuge at 10000r / min for 2min, wash twice in this way, and use the same volume Suspended in sterile water to make a bacterial fluid.

[0031] 3) Take 1ml of bacterial liquid and 20ml containing 100mg / L nano-Fe 3 o 4 6% sodium alginate solution at room temperature and mix well, and then use a syringe to drop the mixture into 4% CaCl at room temperature 2 The solution was cross-linked and calcified at 4°C for 8 hours to obtain calcium alginate gel-immobilized beads, which were washed twice with sterile water and stored at 4°C for late...

Embodiment 3

[0033] Example 3: The application method of immobilized beads in the degradation of ofloxacin.

[0034] First, aerate the immobilized pellets in wastewater for 10 hours for activation. During the activation process, the water temperature is 28±2°C and the aeration rate is 1.5L / min. Then, at a temperature of 28±2°C and a pH value of 6.8-7.2, add Anhydrous piperazine makes piperazine concentration be 50mg / L, under the situation of ofloxacin influent concentration 500 μ g / L, every liter of ofloxacin waste water takes the prepared immobilized pellet of 200ml embodiment 2 to handle , at the same time, the hydraulic retention time is 72h, such as figure 2 Shown, in this case the removal rate of ofloxacin is more than 90%, has better removal effect; And image 3 It shows the removal of ofloxacin for different influent concentrations. The research shows that the removal rate of ofloxacin decreases with the increase of influent concentration. When the concentration of ofloxacin in in...

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Abstract

The invention provides a strain of Ochrobactrum sp. that degrades ofloxacin, which belongs to the technical field of microorganisms. Microbiology Center, the deposit number is CGMCC NO.10539, and the deposit date is February 9, 2015. Under laboratory shaking flask conditions, the degradation rate of the strain to 500ug / L ofloxacin reaches 97.2%, which solves the problem that antibiotic-containing wastewater inhibits microorganisms and affects its treatment effect. The bacterial agent preparation process of the bacterial strain is simple, low in cost, high in efficiency and free from secondary pollution, and has good application prospects.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to an application of a strain of Pallidum bacterium degrading ofloxacin. Background technique [0002] Antibiotics refer to a class of chemical substances with water-soluble and fat-soluble medical characteristics. They can not only kill bacteria, but also have good inhibitory and killing effects on pathogenic microorganisms such as mold, mycoplasma, and chlamydia. They can be used as a treatment for various bacteria. Drugs for infection or infection with pathogenic microorganisms are widely used in the pharmaceutical industry. Usually, antibiotic pollutants have low vapor pressure and low hydrophobicity, and mainly exist in the water environment. The water contains a variety of refractory biotoxic substances and high concentrations of active antibiotics, which inhibit the growth of microorganisms in wastewater biochemical treatment. Therefore, The existing wat...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14C12N11/10C12N11/04C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12N11/04C12N11/10C12N11/14C12N1/205C12R2001/01
Inventor 蔡天明陈吉菲姜灿烂丁大虎许玲一
Owner NANJING AGRICULTURAL UNIVERSITY
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