Production of creatine through fermentation method

Abstract

The invention discloses a production method of creatine through microbial fermentation. In the method, a food-safety microorganism is employed to directly convert a carbon source and a nitrogen source into the creatine through fermentation. The produced creatine reaches a standard of a nutritious supplement and has a wide industrial application prospect. The microorganism in the method includes a corynebacterium glutamicum that is established through gene engineering and is preserved in China General Microbiological Culture Collection Center and is assigned the accession number of CGMCC No.12602.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for producing creatine through microbial fermentation. Background technique [0002] Creatine (Creatine) chemical name is N-methylguanidine acetic acid, also known as guanidinoacetic acid, creatinine, muscle amino acid, methylguanidine acetic acid, etc., is a kind of amino acid derivative naturally produced in vertebrates. A natural nutrient in the human body, creatine in the human body is synthesized by arginine, glycine, and methionine in the liver, kidney, and pancreas, transported to muscle tissue through the blood, and stored in the muscle in the form of phosphocreatine organize. Creatine plays an important role in promoting the synthesis of nucleic acid and protein, increasing the reserve of energy substances in the body, promoting muscle growth, delaying fatigue and accelerating physical recovery. Creatine is currently the most widely used sports nutrit...

AI Technical Summary

Problems solved by technology

The reaction time of the above-mentioned reaction mode is long, the operation process is complicated, and the product purity is not very high

These traditional preparation methods often involve highly toxic cyanamide or the yield is not ideal, which has great potential safety hazards and high production costs. Especially for large-scale industrial production, the leakage of highly toxic substances can easily cause personnel Casualties, but also does not fit the trend of green environmental protection

[0004] Creatine produced by chemical synthesis generally has peculiar smells such as bitterness, and cannot be directly used as a food additive. It often needs to be treated with debittering agents such as sodium thiosulfate. The post-treatment process is complicated, and the yield is only about 50%.