Ultrasonic standing wave type microfluidic chip and manufacturing method thereof

A microfluidic chip and ultrasonic standing wave technology, applied in the field of microfluidics, can solve the problems of manipulating short nanofiber wires, achieve good shape, good effect, and have the effect of ultrasonic standing wave response characteristics

Inactive Publication Date: 2016-11-09
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Manipulation of short and dispersed nanofiber wires in ultraso

Method used

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  • Ultrasonic standing wave type microfluidic chip and manufacturing method thereof
  • Ultrasonic standing wave type microfluidic chip and manufacturing method thereof
  • Ultrasonic standing wave type microfluidic chip and manufacturing method thereof

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0052] Example 1

[0053] The specific process of the preparation and functional modification of PLGA microspheres is as follows:

[0054] (1) Weigh 100mg PLGA and dissolve in 2mLCH 2 Cl 2 Among them, 200μL ultrapure water is used as the internal water phase, the organic phase and the internal water phase are mixed, and ultrasonic treatment is performed for 30s in an ice water bath to form a W / O emulsion; the above W / O emulsion is transferred into 50mL 5% PVA aqueous solution In the ice-water bath, use a homogenizer (6000rpm, 5min) to make the droplets form a W / O / W emulsion with a relatively uniform size; drop the obtained W / O / W emulsion into 80mL 2% isopropanol aqueous solution In a water bath at 39°C, after magnetic stirring for 4 hours, centrifuge (4000rpm, 3min), discard the supernatant, wash the centrifugal residue with 30mL ultrapure water, repeat the centrifugal washing operation three times until the supernatant is clear and colorless. After centrifugation, the centrifugal...

Example Embodiment

[0058] Example 2

[0059] Ultrasonic standing wave microfluidic chip such as Picture 12 As shown, the specific steps are as follows:

[0060] (1) At 76×26mm 2 On the glass slide, fix the rectangular capillary with epoxy glue (length is 50mm, width is 0.2mm, height is 2mm), this rectangular capillary is used as the channel of the microfluidic chip;

[0061] (2) At the outlets on both sides of the rectangular capillary tube, bond polyethylene pipes with an inner diameter of 1mm and an outer diameter of 1.4mm as the input and output of the suspension;

[0062] (3) Finally, on the side of the fixed rectangular capillary, fix the piezoelectric ceramic sheet (15mm×2mm×0.2mm) by epoxy resin glue, and then solder the wires on the front and back sides of the piezoelectric ceramic sheet as the circuit connection wires .

[0063] Take 2 mL of the aqueous solution (0.2 mg / mL) of the product obtained in Example 1 (1) and place it in a 5 mL EP tube as a storage tube. Before the test, the microflui...

Example Embodiment

[0064] Example 3

[0065] Take 500 μL (1 mg) of the PLGA-PEI aqueous solution of the product obtained in Example 1 (2), dilute it to 1 mL with serum-free medium (concentration 0.5 mg / mL), place it in a 5 mL EP tube, and add the serum-free digested and centrifuged HeLa cells (1 million) suspended in the culture medium, with a total volume of 2 mL, were co-cultured at 37°C for 30 minutes to obtain a solution of cell-microsphere conjugates. Before the test, the microfluidic chip was immersed in 2% BSA for 2 hours to build the ultrasonic standing wave microfluidic device platform. First, test whether voltage is applied to both ends of the piezoelectric ceramic, and adjust the output signal of the signal generator to a voltage of 20Vpp , The frequency is 10KHz, the standard is to hear the buzzer after adding this signal, otherwise no signal is added to both ends of the piezoelectric ceramic, then you need to check whether the connector is correct and whether the signal generator outpu...

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Abstract

The invention relates to an ultrasonic standing wave type microfluidic chip and a manufacturing method thereof. According to the chip, particles or cells are controlled, captured and separated in ultrasonic standing waves by PLGA-PEI microspheres or PLGA short nanofiber lines in a chip channel. The method comprises the steps that 1, the PLGA microspheres are synthesized through a double emulsion method and then modified by polyethyleneimine; 2, a PLGA nanofiber membrane is prepared through an electrostatic spinning method and then homogenized to obtain the short nanofiber lines; 3, the ultrasonic standing wave type microfluidic chip is manufactured. According to the chip, the particles or cells can be effectively controlled, the manufacturing process is simple, cost is low, the very high controllability and operability are achieved, and a wide application prospect is achieved.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, in particular to an ultrasonic standing wave microfluidic chip and a preparation method thereof. Background technique [0002] Microfluidics is one of the most cutting-edge technologies in the world. It is a highly interdisciplinary technology that integrates melting, physics, life science, microelectronics, materials, and computers. It is currently mainly used in the fields of chemistry and life science. It is a technique for manipulating or controlling nanoliter or picoliter-level liquids in micron-scale channels, and the entire analysis process from sampling, sample pretreatment, reaction, separation and enrichment to detection is on an integrated microchip Complete, while greatly reducing the amount of samples and reagents, greatly improve the analysis speed, realize the miniaturization, integration and automation of the analysis system, and have the advantages of low cost, high throug...

Claims

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Application Information

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IPC IPC(8): B01L3/00
CPCB01L3/5027B01L3/502707B01L2200/0647B01L2200/12B01L2300/12B01L2300/163B01L2400/0439
Inventor 朱晓玥尹迪魏延传徐刚伟王梦媛史向阳
Owner DONGHUA UNIV
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