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Extraction method of phycobiliprotein of nostoc sphaeroides kutzing

A technology of phycobiliprotein and extraction method of Gexianmi, which is applied in the direction of chemical instruments and methods, decapeptides, peptides, etc., can solve the problem of not considering the edible value of phycobiliprotein, manpower, material resources and energy consumption, and can not guarantee the final To improve the safety of finished products and achieve the effects of increasing phycobiliprotein content, easy storage and cost saving

Inactive Publication Date: 2017-02-22
CHANGDE YANDI BIOTECH LTD CO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can extract phycobiliprotein, the yield is relatively low, only about 7%, and organic reagents are added in the process of use, which cannot guarantee the safety of the final product; common extraction methods are used for research during the extraction process There are many instruments and equipment, and the operation is cumbersome. It does not consider that a large amount of manpower, material resources and energy consumption will be consumed in the actual production process of the enterprise; and most of the researches focus on how to extract and separate phycocyanin from kudzu phycobiliprotein , without considering that phycobiliprotein rich in various nutrients has more food value

Method used

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  • Extraction method of phycobiliprotein of nostoc sphaeroides kutzing
  • Extraction method of phycobiliprotein of nostoc sphaeroides kutzing
  • Extraction method of phycobiliprotein of nostoc sphaeroides kutzing

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Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1 adopts the method of the present invention to carry out laboratory small test crudely extract phycobiliprotein

[0026] Grind the dry matter (moisture content 11%) through the sun or dry water to 60 mesh with a universal pulverizer, and take 21.99g, 23.94g, and 23.35g of the pueraria coarse powder and place them in a beaker Add 1100 g of purified water, let it stand at room temperature for 6 hours, centrifuge at 8000 r / min for 15 min, filter to obtain a crude extract, and freeze-dry the crude extract to obtain a crude extract of phycobiliproteins from Pueraria japonica. The results are shown in Table 1 below:

[0027] Table 1

[0028]

[0029]

[0030] It can be seen from the above table 1 that the yield of the crude extract obtained from the three extractions is relatively high, all of which are more than 20%, and the average value is 22.5%. It is verified that the method of the present invention has good reproducibility through three repeated expe...

Embodiment 2

[0031] Embodiment 2 adopts the method of the present invention to carry out laboratory small test crude extraction phycobiliprotein

[0032] Grind the dry matter (moisture content 11%) through the sun or dry water to 80 mesh with a universal pulverizer, and take 25.12g, 25.30g, and 25.60g of the pueraria coarse powder and place them in a beaker Add 1260g of purified water, let it stand at room temperature for 6 hours, centrifuge at 8000r / min for 15min, filter to obtain a crude extract, freeze-dry the crude extract to obtain a crude extract of phycobiliprotein from Pueraria japonica. The results are shown in Table 2 below:

[0033] Table 2

[0034]

[0035] It can be seen from the above table 2 that the yield of the crude extract obtained from the three extractions is relatively high, all of which are more than 10%, and the average value is 11.1%. It is verified that the method of the present invention has good reproducibility through three repeated experiments.

Embodiment 3

[0036] Embodiment 3 adopts the method of the present invention to carry out the crude extraction phycobiliprotein of laboratory small test

[0037] Grind the dry matter (moisture content 11%) through the sun or dry water to 20 mesh with a universal pulverizer, and take 24.87g, 25.45g, and 25.00g of the pueraria coarse powder and place them in a beaker Add 1260g of purified water, let it stand at room temperature for 6 hours, centrifuge at 8000r / min for 15min, filter to obtain a crude extract, freeze-dry the crude extract to obtain a crude extract of phycobiliprotein from Pueraria japonica. The results are shown in Table 3 below:

[0038] table 3

[0039]

[0040]

[0041] It can be seen from the above table 3 that the yield of the crude extract obtained by three extractions is relatively high, all of which are more than 10%, and the average value is 13.2%. It is verified that the method of the present invention has good reproducibility through three repeated experiment...

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Abstract

The invention relates to an extraction method of phycobiliprotein of nostoc sphaeroides kutzing. The extraction method comprises the following steps of crushing dry nostoc sphaeroides kutzing to be 20 to 80 meshes, adding purified water of which the weight is 40 to 60 times the weight of the crushed dry nostoc sphaeroides kutzing to extract the phycobiliprotein for 4h to 8h, filtering an obtained mixture, and carrying out freeze drying on a filtrate, so that the phycobiliprotein is obtained. According to the method provided by the invention, the phycobiliprotein is directly extracted by using 20 to 80 meshes of nostoc sphaeroides kutzing dry powder; the extraction process is simplified; compared with a method for extracting the phycobiliprotein by adopting multigelation, ultrasonic wall breaking, soaking by a phosphate buffer solution and salting out by ammonium sulfate, the extraction method is simple and is easy to operate; further, the yield of a phycobiliprotein crude extract of the nostoc sphaeroides kutzing is improved; the content of the crude extract phycobiliprotein is improved. By using the method provided by the invention, a simpler, more practical, cost-saving and safe technical process which is capable for industrialized mass production is provided for a production enterprise.

Description

technical field [0001] The invention relates to a convenient and efficient method for crudely extracting phycobiliproteins from Gexianmi phycobiliproteins. Background technique [0002] Nostoc sphaeroides Kutzing (Nostoc sphaeroides Kutzing), also known as Tianxian rice and Tianxiancai, is an edible nitrogen-fixing cyanobacteria and a group of algae. Its algal bodies are rich in protein, 8 kinds of amino acids needed by the human body, algae starch, folic acid, soluble fiber, multivitamins and 15 kinds of minerals, the most abundant are phosphorus, sulfur, calcium, potassium, iron, etc., and trace elements are Zinc, copper, manganese, selenium, its high calcium content is extremely rare in vegetables, and it is a food with high calcium, low fat and balanced nutrition. According to "Compendium of Materia Medica" and "Supplements to Compendium of Materia Medica", Gexianmi can treat night blindness, rectal prolapse, burns and scalds. In addition, according to the "National Co...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K1/14
CPCC07K14/795
Inventor 刘红英安彦君钟责平董美丽张瑞华王远忠陈牧
Owner CHANGDE YANDI BIOTECH LTD CO
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