Aqueous phase preparation method of red light silver sulfide quantum dots

Abstract

The invention discloses an aqueous phase preparation method of red light silver sulfide quantum dots and belongs to the cross technical field of material science and photonics. The aqueous phase preparation method concretely comprises the following steps: taking silver nitrate and sodium sulfide as reaction precursor materials and desoxyribonucleic acid as a stabilizer, reacting, precipitating and producing nano crystals in an aqueous liquid, and obtaining silver sulfide quantum dots which can be stably dispersed in the aqueous liquid by virtue of a centrifugal separation method. The method disclosed by the invention adopts water as a solvent, the reaction temperature is mild and controllable, the size of the obtained silver sulfide quantum dot product is 1-10 nanometers, and fluorescence emission is located in a red band, so that the aqueous phase preparation method is applicable to fluorescence microscopy imaging detection of in vitro cells and has broad application prospects in the aspect of biological detection.

Description

technical field [0001] The invention belongs to the interdisciplinary research field of materials science and photonics, and relates to a water-phase preparation method of red light silver sulfide quantum dots. Background technique [0002] Semiconductor quantum dots are usually nanoparticles composed of II-VI or III-V elements, and the particle size is generally between 1nm and 10nm. Because its size is smaller than or close to the exciton Bohr radius, the electrons and space inside the material The hole is quantum-confined, and the continuous energy band structure becomes a discrete energy level structure with molecular characteristics, which can emit fluorescence after being excited. [0003] Compared with traditional traditional organic fluorescent dyes, quantum dots have the advantages of wide absorption spectrum, narrow emission spectrum, no photobleaching, and easy surface modification. Quantum dots are sufficiently small in size to allow them to enter cells by diffu...

AI Technical Summary

Problems solved by technology

Biological cells will produce strong autofluorescence under the excitation of purple light and ultraviolet light. Therefore, it is difficult to obtain imaging results with high signal-to-noise ratio with blue-green fluorescent quantum dot materials as probes.

Due to the weak absorption and scattering of light in the near-infrared band by biological tissues, near-infrared fluorescent imaging probes have great advantages in live animal imaging applications, but in most in vitro cell imaging studies, depth imaging capabilities are not imaging The limiting factor of the results, while the fluorescence signal acquisition system of most existing commercial fluorescence microscopy imaging systems works in the visible light band, limits the application of near-infrared quantum dots as fluorescent probes in ex vivo cells

[0004] The traditional red light quantum dots are mainly cadmium-based semiconductor quantum dots such as cadmium selenide and cadmium telluride. The biological toxicity and environmental pollution of cadmium greatly limit the application of cadmium-based quantum dots. Therefore, the development of cadmium-free red light quantum dots , is of great significance

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