A kind of boar feed and preparation method thereof
A boar and feed technology, applied in animal feed, animal feed, bacteria, etc., can solve problems such as insufficient attention to boar nutrition, decline in boar reproductive performance, and inability to produce sperm normally, so as to enhance the body's anti-stress and Effects of disease resistance, improvement of reproductive performance, and improvement of acrosome integrity
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Embodiment 1
[0031]Example 1 Preparation of Lactobacillus acidophilus CGMCC No.6499 bacterial powder
[0032] 1) Preparation of Lactobacillus acidophilus seed liquid: Inoculate the strains preserved on the slant of the test tube into an anaerobic tube containing 20 mL of MRS medium, and culture at a constant temperature for 16-24 hours at 37°C until the number of viable bacteria reaches 10 7 CFU / mL, inoculate at 1% in a 250mL Erlenmeyer flask filled with 100mL seed medium, culture anaerobically at 37°C for 16-24h, until the number of viable bacteria reaches 10 7 CFU / mL or more, as a seed solution for later use.
[0033] Seed liquid MRS medium is: peptone, 10.0g / L; beef extract, 8.0g / L; yeast extract, 4.0g / L; glucose, 20.0g / L; Tween 80, 1mL / L; dipotassium hydrogen phosphate, 2.0g / L; Sodium acetate trihydrate, 5.0g / L; Triammonium citrate, 2.0g / L; Magnesium sulfate heptahydrate, 0.2g / L; Manganese sulfate, 0.05g / L; Add distilled water to 1000mL, pH 6.2 ±0.2.
[0034] 2) Liquid submerged fer...
Embodiment 2
[0040] Example 2 Screening of Bacillus subtilis DZS11
[0041] 1. Primary screening
[0042] Collect 10 piglet feces samples, weigh 10g soil samples respectively, add 90mL sterile water to make bacterial suspension, treat in 80°C water bath for 20min, vibrate at 180r / min for 30min, gradually dilute to a suitable gradient, and spread on LB On the culture medium, 86 suspected bacillus strains were obtained.
[0043] Milk medium (for primary screening of protease): 5g skimmed milk dissolved in 50ml distilled water; 1.5g agar dissolved in 50ml distilled water, the two liquids were sterilized separately, when cooled to 45-50°C, the two liquids were mixed and poured on the plate , Serve with a milk tablet. Invert the plate overnight to dry the surface moisture, then spot the bacteria on the plate, mix and invert the plate, and set aside overnight. When preparing this medium, do not mix milk and agar to sterilize to prevent the milk from coagulating.
[0044] Starch hydrolysis pl...
Embodiment 3
[0066] Embodiment 3 Bacillus subtilis DZS11 bacteria powder preparation
[0067] Fermentation of Bacillus subtilis DZS11: The fermentation medium used is prepared as follows: 0.5% corn flour, 1% bean cake powder, 0.4% sucrose, 0.6% fish meal, 0.1% calcium carbonate, 0.1% potassium dihydrogen phosphate, 0.2% magnesium sulfate, sulfuric acid Ferrous 0.025%, manganese sulfate 0.025%. Prepare the fermented liquid in proportion, then adjust the pH to 7.2. After sterilizing at 121°C for 30 minutes, cool down to 37°C, inoculate seed solution with a bacterial age of 16 hours, inoculum amount of 3%, 37°C, keep the rotation speed at 300rpm, air ratio 1:0.4, tank pressure 0.05. Cultivate to 18h as the end point of fermentation, put the tank, and the number of viable bacteria of Bacillus subtilis DZS11 is 6.21×10 9 CFU / mL.
[0068] Spray-dried protective agent (0.8% of glycerol, 0.2% of sodium glutamate, 19% of cornstarch) was added to the fermented liquid, and then spray-dried at high...
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