Enzyme degradation-controllable anti-nonspecific protein adsorption polypeptide and monomer and preparation method thereof

A technology of monomers and inhibitors, applied in the field of peptides and their preparation, can solve the problems of large impact on the size of biomolecules, abnormal cell death, loss of protein non-adsorption, etc., achieve broad application prospects, and improve resistance to non-specific proteins The effect of adsorption capacity
CN106565824APending Publication Date: 2017-04-19ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2017-04-19

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Abstract

The invention discloses enzyme degradation-controllable anti-nonspecific protein adsorption polypeptide and a monomer and a preparation method thereof. By means of polycondensation of a dipeptide monomer containing protected carboxyl group and protected amino group at the side chain and subsequent deprotection, polypeptide with perfect repetitive positive and negative charges is prepared, and anti-nonspecific protein adsorption capacity of polypeptide is enhanced. Further, by polycondensation of a dipeptide monomer containing protected carboxyl group and protected amino group at the side chain and an amino acid monomer modified with protected carboxyl group and protected amino molecules simultaneously contained at the side chain at different ratios and subsequent deprotection, anti-nonspecific protein adsorption polypeptide with different enzyme degradation half-life is obtained, and regulation and control of enzyme degradation capability of the anti-nonspecific protein adsorption polypeptide is realized. By controlling the ratio of an end-capping reagent to a monomer, molecular weight of the target polypeptide is regulated and controlled. By the utilization of functional modification groups on the end-capping reagent, modification of different proteins, polypeptide and other polymer and different material surfaces can be realized.
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Description

technical field

[0001] The invention relates to a polypeptide and a preparation method thereof, in particular to an anti-nonspecific protein adsorption polypeptide and a preparation method thereof. Background technique

[0002] Anti-non-specific protein adsorption materials (nonfouling materials) is a special class of biocompatible materials proposed in the last ten years, and was listed as an annu.rev.biomed.eng. A new generation of biocompatible materials. By interacting with water, it prevents the adsorption of protein molecules, thereby avoiding further biochemical reactions, such as blood coagulation in blood and other problems. Through continuous and in-depth research on non-specific protein adsorption materials, it is found that anti-non-specific protein adsorption materials can not only effectively prevent the coagulation process in the blood contact system, but also can be applied to prevent the adsorption process of biological macromolecules at a wide range of sol...

Claims

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