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Process for preparing high-purity xylooligosaccharide

A xylo-oligosaccharide, high-purity technology, applied in biochemical equipment and methods, microorganisms, fermentation and other directions, can solve problems such as reducing product yield, and achieve the effects of reducing energy consumption, good moisture absorption resistance and light color.

Pending Publication Date: 2017-05-10
SHANDONG LONGLIVE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Xylo-oligosaccharide preparation process The xylo-oligosaccharide process preparation methods 200410023875.X and 200410013840.8 have been published. The content of xylo-oligosaccharide in the enzymolysis solution of the two is 70% to 76%. The content of xylan is more than 90%, which contains 2% to 5% of monosaccharides, 1% to 5% of heptasaccharides or macromolecular substances above heptasaccharides; patent 201210136672.6 uses chromatographic separation technology to realize xylooligosaccharides, xylose Although the separation of the three components of sugar and arabinose is lower than nanofiltration water consumption and energy consumption in the whole production process, the whole process still needs to invest in new equipment. However, the yield of xylooligosaccharides in the purification process is only 80%, the rest is separated into the xylose liquid, which directly reduces the product yield

Method used

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  • Process for preparing high-purity xylooligosaccharide
  • Process for preparing high-purity xylooligosaccharide
  • Process for preparing high-purity xylooligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Crush the corncobs to about 20 meshes, adjust the slurry according to the solid-to-liquid ratio of 1:10, and pass through NH 3 Soak to pH 9.0 at 50°C for 24 hours, drain the pipe filter, and use the filtrate to soak the next batch of materials. After adding phosphoric acid to adjust the pH to 3.5, feed the materials directly into the horizontal tube continuous steamer, cook at 0.55MPa for 90 minutes, and release the pressure. into the enzymatic tank. After spraying, when the temperature drops to 60-65°C, add xylanase and dextranase complex, the enzyme activity ratio is 20-5:1, the amount of xylanase added is 5IU / g, and the enzyme is hydrolyzed at 40-60°C After 4 hours, lower the temperature to 37° C., inoculate Pichia stipitis CICC1960 and ferment for 36 hours. When the glucose concentration is less than 0.5%, raise the temperature to 80-100° C. to kill the enzyme. According to the conventional method including activated carbon decolorization and ion exchange resin rem...

Embodiment 2

[0071] After the corn cob is crushed, mix it with a sodium hydroxide solution with a mass concentration of 0.05% in a mass ratio of 1:7 to pH 10, heat up to 60 degrees Celsius and keep it warm for 30 minutes, filter out the liquid; wash it with water until the pH value is neutral; mix with process water Mix according to the mass ratio of 1:3, add corncob dry weight percentage of 0.7% acetic acid, steam explosion treatment, treatment pressure 1.5MPa, time 120s; xylose content in the steam explosion liquid is 5.85%, when the temperature is reduced to 60~ Add xylanase and dextranase complex at 65°C, the enzyme activity ratio is 20-5:1, the amount of xylanase added is 8IU / g, and the temperature is lowered to 37 after enzymolysis at 40-60°C for 4 hours. °C, inoculate Torulopsis albicans (CICC31239) or Candida parapsilosis CICC31239 and ferment for 72 hours, and when the glucose concentration is ≤0.5%, heat up to 80-100 °C to inactivate the enzyme. Add 0.8g xylanase composition to c...

Embodiment 3

[0084] Crush the corncobs to about 20 meshes, adjust the slurry according to the solid-to-liquid ratio of 1:10, and pass through NH 3 Soak to pH 9.0 at 50°C for 24 hours, drain the pipe filter, and use the filtrate to soak the next batch of materials. After adding phosphoric acid to adjust the pH to 3.5, feed the materials directly into the horizontal tube continuous steamer, cook at 0.55MPa for 90 minutes, and release the pressure. into the enzymatic tank. After spraying, when the temperature drops to 60-65°C, add xylanase and dextranase complex, the enzyme activity ratio is 20-5:1, the amount of xylanase added is 5IU / g, and the enzyme is hydrolyzed at 40-60°C After 4 hours, lower the temperature to 37°C, inoculate Pichia stipitis CICC1960 and Candida parapsilosis CICC1257, the inoculum amount is 1% (v / v), and inoculate 1% (v / v) Torulopsis CICC31239 after 16 hours of fermentation After 20 hours of fermentation, when the glucose concentration is less than 0.5%, the temperatur...

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Abstract

The invention discloses a process for preparing high-purity xylooligosaccharide. The process comprises the following steps: firstly, removing acetyl and monosaccharide of the side chain in a hemicellulose raw material through deacetylation; performing pretreatment, enzymolysis and fermentation to enable the purity of xylooligosaccharide to be more than or equal to 90 percent. An xylooligosaccharide product prepared by the method has light color and stable pH value. The process can be used for greatly reducing energy consumption, improving product yield and reducing pollutant emission, is more friendly to the environment, can be widely taken by diabetic patients and hyperglycemia patients, and is a novel technology for preparing xylooligosaccharide with high-value transformation.

Description

technical field [0001] The invention relates to the field of food processing, relates to a preparation process of xylooligosaccharides, and belongs to the technical field of functional sugar alcohol production. Background technique [0002] Xylo-oligosaccharides are a kind of oligosaccharides, which are formed by β-1,4-endoxylanase with xylan as a substrate and hydrolyze β-1,4 glycosidic bonds with a degree of polymerization of 2-7 oligosaccharides, of which xylobiose and xylotriose are the main active ingredients. Because xylooligosaccharides can improve the balance of the digestive tract flora of organisms (humans and animals), promote the growth of beneficial bacteria in the digestive tract, inhibit the reproduction of harmful microorganisms, promote nutrient absorption, and improve the body's immunity, it is currently widely used in food, health care food and feed field. Not only that, xylooligosaccharides are low in sweetness and low in calories, difficult to be diges...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12P19/00C12P19/12C12R1/84C12R1/645C12R1/72
CPCC12P19/00C12P19/04C12P19/12C12P2203/00
Inventor 程少博肖林孔令军夏蕊蕊朱艳杰刘宪夫覃树林李莹
Owner SHANDONG LONGLIVE BIO TECH CO LTD
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