PELCL/polycaprolactone-REDV electrospun fiber film and preparation method

A technology of electrospun fiber and caprolactone, which is applied in the field of tissue engineering and biomedical materials, can solve the problems of increasing the cost of raw materials and reducing the activity of biologically active substances, and achieves good fiber morphology, good biological activity, and promotes the development of vascular endothelial cells. Effects of Adhesion and Growth

Inactive Publication Date: 2017-05-31
TIANJIN UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, by surface chemical modification, coupling small peptides on the surface of electrospun fiber membranes loaded with bioactive substanc

Method used

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  • PELCL/polycaprolactone-REDV electrospun fiber film and preparation method
  • PELCL/polycaprolactone-REDV electrospun fiber film and preparation method
  • PELCL/polycaprolactone-REDV electrospun fiber film and preparation method

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preparation example Construction

[0034] The preparation method of the above-mentioned polyethylene glycol-b-poly(L-lactide-co-ε-caprolactone) (PELCL) / polyε-caprolactone-REDV electrospun microfiber membrane, is characterized in that Including the following process:

[0035] (1) Polyethylene glycol-b-poly(L-lactide-co-ε-caprolactone) (PELCL) and polyε-caprolactone-REDV, dissolved together in chloroform and N,N-dimethyl The electrospinning solution with a concentration of 100-200 mg / mL is formulated in a mixed solvent with a volume ratio of 4:1-8:1 of phenyl formamide, and the mass ratio of PELCL and polyε-caprolactone-REDV is (5-1 ):1;

[0036] (2) Electrospinning the solution obtained in step (1), the electrospinning conditions are as follows: the flow rate of the solution is 0.02~0.10mL / h, the voltage of electrospinning is 12~20kV, and the receiving distance is 15~20cm, and 50~20cm is obtained. Electrospun fiber membrane with a thickness of 200 μm, that is, polyethylene glycol-b-poly(L-lactide-co-ε-caprolac...

Embodiment 1

[0038] In a three-neck flask equipped with magnetic stirring, 3 g of polyε-caprolactone ( Two-terminal hydroxyl) was dissolved in 10mL of anhydrous dichloromethane, according to the molar ratio [two-terminal hydroxyl polyε-caprolactone] / [CDI]=1:2, add CDI 74.5mg, in N 2 Stir the reaction at room temperature for 24 hours, then add 0.52 g of hydroxyethyl acrylate and 0.1 g of catalyst 4-lutidine, stir and react for 48 hours at room temperature, the product is precipitated with excess methanol, and dried in vacuum for 24 hours to obtain white polyε - Caprolactone double bond modified product.

[0039] Dissolve 796 mg of the double bond modified product of polyε-caprolactone in 3 mL of dichloromethane solution. Add 40mg of REDV at 1:1, then add 8.4mg of photocatalyst 2,2-dimethoxy-2-phenylacetophenone, stir evenly at room temperature, place in a circular petri dish, and irradiate in 365nm UV for 10min , the product was precipitated with ethanol to obtain polyε-caprolactone-REDV...

Embodiment 2

[0042] In a three-neck flask equipped with magnetic stirring, 5 g of polyε-caprolactone ( Two-terminal hydroxyl) was dissolved in 10mL of anhydrous dichloromethane, according to the molar ratio [two-terminal hydroxyl polyε-caprolactone] / [CDI]=1:2, add CDI 32.4mg, in N 2 Stir the reaction at room temperature for 24 hours, then add 0.60 g of hydroxyethyl acrylate and 0.11 g of catalyst 4-lutidine, stir and react at room temperature for 60 hours, the product is precipitated with excess methanol, and dried in vacuum for 24 hours to obtain white polyε - Caprolactone double bond modified product.

[0043] Dissolve 600 mg of the double bond modified product of polyε-caprolactone in 3 mL of dichloromethane solution. Add REDV-SH 15mg at 1:1, then add 3.1mg of photocatalyst 2,2-dimethoxy-2-phenylacetophenone, stir evenly at room temperature, place in a circular petri dish, in 365nm UV After irradiating for 15 minutes, the product was precipitated with ethanol to obtain polyε-caprolac...

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Abstract

The invention relates to a polyethylene glycol-b-poly(L-lactide-co-epsilon-caprolactone)/poly (epsilon-caprolactone)-REDV blended superfine electrospun fiber film and a preparation method thereof. Poly (epsilon-caprolactone) with low molecular weight is coupled with REDV small peptides, poly (epsilon-caprolactone)-g-REDV is prepared, poly (epsilon-caprolactone)-g-REDV and polyethylene glycol-b-poly(L-lactide-co-epsilon-caprolactone) with high molecular weight are together dissolved in a mixed solvent of chloroform and N,N-dimethyl formamide, and an electrostatic spinning method is used for preparation in order to obtain the superfine fiber film. The electrospun fiber film comprises superfine fiber whose diameter is 400-1000nm, and thickness is 50-200[mu]m. The electrospun fiber film contains REDV small peptides on the surface, in order to promote adhesion and growth functions of vascular endothelial cells; at the same time, the superfine fiber film is used for wrapping and loading nucleic acid, protein, medicament and other bioactive substances, and has capability for controlled release of bioactive substances. The product is used for biomedical materials.

Description

technical field [0001] The invention relates to a polyethylene glycol-b-poly(L-lactide-co-ε-caprolactone) (PELCL) / polycaprolactone-REDV electrospun ultrafine fiber membrane and its preparation method, belonging to in the field of tissue engineering and biomedical materials. Background technique [0002] Using electrospinning technology to load bioactive substances such as gene proteins in tissue engineering scaffolds can regulate cell adhesion, proliferation and migration, and is beneficial to tissue repair and regeneration. Biodegradable polylactide copolymer polyethylene glycol-b-poly(L-lactide-co-ε-caprolactone) (PELCL), polyε-caprolactone (polycaprolactone, PCL), etc. Good biocompatibility and excellent mechanical properties. Electrospinning technology was used to prepare ultrafine fiber membranes, which simulated the structure of extracellular matrix and could be used as scaffold materials for tissue reconstruction. Arginine-glutamic acid-aspartic acid-valine (Arg-Gl...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/22A61L27/54C08G63/08C08G63/91C08G81/00D04H1/4382D04H1/728
CPCA61L27/18A61L27/22A61L27/54A61L2300/252A61L2300/412A61L2300/602C08G63/08C08G63/912C08G81/00D04H1/4382D04H1/728C08L67/04C08L87/00
Inventor 袁晓燕周芳任丽霞赵蕴慧崔策文美玲
Owner TIANJIN UNIV
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